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学科主题: 临床医学
题名:
Gossypol repressed the gap junctional intercellular communication between Sertoli cells by decreasing the expression of Connexin43
作者: Zhou, De-Rong1,2; Zhou, Yong-Cui2; Cui, Guang-Hui1; Guo, Xin1; Qin, Jie1; Gui, Yao-Ting1; Cai, Zhi-Ming1
关键词: Gossypol ; Sertoli cells ; Gap junctional intercellular communication ; Connexin43
刊名: TOXICOLOGY IN VITRO
发表日期: 2008-10-01
DOI: 10.1016/j.tiv.2008.07.012
卷: 22, 期:7, 页:1719-1725
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Toxicology
研究领域[WOS]: Toxicology
关键词[WOS]: MICE ; SPERMATOGENESIS ; KNOCKOUT ; PROTEIN
英文摘要:

Previous studies showed that gossypol could block the gap junctional intercellular communication (GJIC) between cultured cells. The present study was designed to investigate the effects of gossypol on the GJIC and the expression of connexin43 (Cx43) in the cultured cells. A Sertoli cell line, TM4, was treated with different concentrations of gossypol 1.25, 2.5, 5, and 10 mu mol/L for 6, 12, 24, and 48 h. Cell viability was assessed with CCK-8 assay. GJIC in the cells was determined using the scrape loading and dye transfer (SLDT) assay; the expression of Cx43 was detected by RT-PCR, immunofluorescence and Western blot analysis. The SLDT assay showed gossypol significantly decreased GJIC between adjacent cells. RT-PCR, immunofluorescence and Western blot analyses demonstrated the expression of Cx43 in TIV14 cells. The expression of Cx43 was gradually decreased with the increasing concentrations of gossypol, and the effect occurred as early as 6 h after the treatment and continued until 48 In. These results suggested that gossypol impaired GJIC by decrease of Cx43 expression in the cells, which is important for Sertoli cells to regulate spermatogenesis. (C) 2008 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 30770810 ; 30700824 ; 30500543 ; 200713060401061 ; 2008CB517412
项目资助者: National Natural Science Foundation of China ; Science Foundation of Guangdong Province
WOS记录号: WOS:000260290100007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60507
Appears in Collections:北京大学深圳医院_生殖医学科_期刊论文

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作者单位: 1.Peking Univ, Shenzhen Hosp, Ctr Reprod Med, Lab Male Reprod Med, Shenzhen 518036, Peoples R China
2.Shantou Univ, Coll Med, Shantou 515041, Peoples R China

Recommended Citation:
Zhou, De-Rong,Zhou, Yong-Cui,Cui, Guang-Hui,et al. Gossypol repressed the gap junctional intercellular communication between Sertoli cells by decreasing the expression of Connexin43[J]. TOXICOLOGY IN VITRO,2008,22(7):1719-1725.
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