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学科主题: 药学
题名:
Cerebral nuclei distribution study of dehydrodiisoeugenol as an anxiogenic agent determined by RP-HPLC
作者: Zhang, You-Bo1,2; Zhu, Li-Qiao3; Yang, Xiu-Wei1,2
关键词: Myristica fragrans Houtt ; Nutmeg ; Dehydrodiisoeugenol ; Cerebral nuclei distribution ; Column liquid chromatography
刊名: FITOTERAPIA
发表日期: 2013
DOI: 10.1016/j.fitote.2012.09.022
卷: 84, 页:47-53
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Medicinal ; Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: MYRISTICA-FRAGRANS SEEDS ; MACE
英文摘要:

A sensitive RP-HPLC-DAD method was established to quantify dehydrodiisoeugenol (DDIE) in rat cerebral nuclei. The assay procedure involved one-step extraction of DDIE and daidzein, as an internal standard, from rat plasma and various cerebral nuclei with ethyl acetate. Chromatographic separation was performed on a Diamonsil (TM) DOS C-18 column with methanol-water (81:19, v/v) as a mobile phase. The UV absorbance of the samples was measured at the wavelength of 270 nm. The analysis method was proved to be precise and accurate at linearity ranges in plasma and each cerebral nucleus with correlation coefficients of >= 0.9971. The results indicated that the method established was successfully applied to cerebral nuclei distribution study of DDIE after intravenous administration at a single dose of 40 mg/kg to rat. DDIE showed high concentration in all of cerebral nuclei at 8 min, which indicated that DDIE could cross the blood-brain barrier rapidly and might be one of the main bioactive substances of nutmeg. The results provide fundamental data for evaluating the effects of DDIE on the central nervous system and to be developed into an effective anxiogenic agent. (c) 2012 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 2011BAI07B08 ; 30973863 ; 81161120429 ; 2009ZX09301-010 ; Z0004105040311
项目资助者: National Key Technology Research and Development Program of China ; National Natural Science Foundation of China ; "Major New Medicine Project" in Mega-projects of Science Research of China ; Beijing Municipal Special-Purpose Science Foundation of China
WOS记录号: WOS:000316531400008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60528
Appears in Collections:北京大学药学院_天然药物学系_期刊论文

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作者单位: 1.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100871, Peoples R China
2.Peking Univ, Dept Nat Med, Sch Pharmaceut Sci, Hlth Sci Ctr, Beijing 100871, Peoples R China
3.Shandong Univ Tradit Chinese Med, Jinan, Peoples R China

Recommended Citation:
Zhang, You-Bo,Zhu, Li-Qiao,Yang, Xiu-Wei. Cerebral nuclei distribution study of dehydrodiisoeugenol as an anxiogenic agent determined by RP-HPLC[J]. FITOTERAPIA,2013,84:47-53.
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