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学科主题: 基础医学
题名:
Modulation of cellular immunity by antibodies against calreticulin
作者: Qiu, Xiang2; Hong, Chao1; Zhong, Zhaoyan2; Li, Yue2; Zhang, Tengteng1; Bao, Wanrong1; Xiong, Sidong1; Gao, Xiao-Ming1,2
关键词: Anti-CRT antibodies ; Calreticulin ; Immunoregulation
刊名: EUROPEAN JOURNAL OF IMMUNOLOGY
发表日期: 2012-09-01
DOI: 10.1002/eji.201142320
卷: 42, 期:9, 页:2419-2430
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology
研究领域[WOS]: Immunology
关键词[WOS]: HEAT-SHOCK PROTEINS ; SYSTEMIC-LUPUS-ERYTHEMATOSUS ; ANTIGEN-PRESENTING CELLS ; SURFACE CALRETICULIN ; APOPTOTIC CELLS ; DENDRITIC CELLS ; T-CELLS ; MOLECULAR CHAPERONE ; ACTIVATION ; RECEPTOR
英文摘要:

Although caltreticulin (CRT) is mainly a residential ER protein, it is also expressed on the membrane surface of various types of cells exhibiting multiple functions. We report here that intraperitoneal administration of a soluble recombinant CRT fragment (rCRT/39-272) led to a substantial decrease in delayed type hypersensitivity (DTH) responses in BALB/c mice and EAE in C57BL/6 mice. In the recall response against keyhole limpet hemocyanin (KLH) in vitro, draining lymph node cells from the rCRT/39-272-treated mice produced less IFN-? but more IL-4 as compared with the cells from the control group. The immunomodulating effect of intraperitoneally administered rCRT/39-272 was attributed to anti-CRT Abs thereby induced, because, in passive transfer experiments, the CRT-specific antiserum could suppress DTH in BALB/c mice. B-cell-deficient mu MT mice were not susceptible to rCRT/39-272-mediated DTH suppression. Furthermore, CRT appears on the surface of murine T cells soon after activation and remains detectable (at relatively low level) by flow cytometry for approximately 5 days in vitro. Anti-CRT Abs were able to inhibit AKT phosphorylation, proliferation, and cytokine production by activated murine T cells. We propose that cell surface CRT could play a role in the function of effector T cells and may be considered a target for immunological manipulation.

语种: 英语
所属项目编号: IRT1075 ; 30890142/31070781 ; 2010CB529102
项目资助者: PCSIRT ; National Foundation of Natural Science of China ; National Key Basic Research Programs ; Priority Academic Program Development of Jiangsu Province Higher Education Institutions
WOS记录号: WOS:000308404800024
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60555
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Soochow Univ, Inst Biol & Med Sci, Suzhou 215123, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100871, Peoples R China

Recommended Citation:
Qiu, Xiang,Hong, Chao,Zhong, Zhaoyan,et al. Modulation of cellular immunity by antibodies against calreticulin[J]. EUROPEAN JOURNAL OF IMMUNOLOGY,2012,42(9):2419-2430.
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