学科主题临床医学
Dendritic cells from chronic hepatitis B patients can induce HBV antigen-specific T cell responses
Li, Ruo-Bing1; Chen, Hong-Song1; Xie, Yao2; Fei, Ran1; Cong, Xu1; Jiang, Dong1; Wang, Song-Xia1; Wei, Lai1; Wang, Yu1
刊名WORLD JOURNAL OF GASTROENTEROLOGY
2004-06-01
10期:11页:1578-1582
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
资助者"973" Program ; National Natural Science Foundation of China ; "863" Program ; "973" Program ; National Natural Science Foundation of China ; "863" Program
研究领域[WOS]Gastroenterology & Hepatology
英文摘要

AIM: To determine whether dendritic cells (DCs) from chronic hepatitis B patients could induce HBV antigen-specific T cell responses or not.

METHODS: DCs were generated from peripheral blood mononuclear cells of patients with chronic hepatitis B (CHB) infection and healthy donors. We compared the phenotypes of these DCs and their ability to secrete cytokines and to participate in mixed lymphocyte reactions. In addition, autologous lymphocytes were cultured with DCs loaded with HBV core region peptide HBcAg8-27, an epitope recognized by cytotoxic T lymphocytes (CTL), and bearing human leucocyte antigen (HLA)-A2 for 10 d. Cytokine secretion and lytic activity against peptide-pulsed target cells were assessed.

RESULTS: DCs with typical morphology were generated successfully by culturing peripheral blood mononuclear cells (PBMCs) from CHB patients with AIM-V containing GM-CSF and IL-4. Compared with DCs from normal donors, the level of CD80 expressed in DCs from CHB patients was lower, and DCs from patients had lower capacity of stimulate T cell proliferation. When PBMCs isolated from patients with chronic or acute hepatitis B infection and from normal donors were cocultured with HBcAg18-27 peptide, the antigen-specific memory response of PBMCs from acute hepatitis B patients was stronger than that of PBMCs from chronic hepatitis B patients or normal donors. PBMCs cocultured with DCs treated with HBcAg18-27 CTL epitope peptide induced an antigen-specific T cell reaction, in which the level of secreted cytokines and lytic activity were higher than those produced by memory T cells.

CONCLUSION: DCs from patients with CHB can induce HBV antigen-specific T cell reactions, including secretion of cytokines essential for HBV clearance and for killing cells infected with HBV.

语种英语
所属项目编号G1999054106 ; 30170047 ; 2001AA217151 ; 2002AA217071
资助者"973" Program ; National Natural Science Foundation of China ; "863" Program ; "973" Program ; National Natural Science Foundation of China ; "863" Program
WOS记录号WOS:000208097000010
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60563
专题北京大学第二临床医学院_北京大学肝病研究所
作者单位1.Ditan Hosp, Beijing 100011, Peoples R China
2.Peking Univ, Peoples Hosp, Inst Hepatol, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Li, Ruo-Bing,Chen, Hong-Song,Xie, Yao,et al. Dendritic cells from chronic hepatitis B patients can induce HBV antigen-specific T cell responses[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2004,10(11):1578-1582.
APA Li, Ruo-Bing.,Chen, Hong-Song.,Xie, Yao.,Fei, Ran.,Cong, Xu.,...&Wang, Yu.(2004).Dendritic cells from chronic hepatitis B patients can induce HBV antigen-specific T cell responses.WORLD JOURNAL OF GASTROENTEROLOGY,10(11),1578-1582.
MLA Li, Ruo-Bing,et al."Dendritic cells from chronic hepatitis B patients can induce HBV antigen-specific T cell responses".WORLD JOURNAL OF GASTROENTEROLOGY 10.11(2004):1578-1582.
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