|Stro-1(+) stromal cells have stem-like features in giant cell tumor of bone|
|Lan, Jie1,2; Liu, Xiaoguang1; Rong, Wei1,2; Wei, Feng1; Jiang, Liang1; Yu, Haiyan2; Dang, Gengting1; Liu, Zhongjun1|
|关键词||giant cell tumor of bone neoplastic stem cells Stro-1 self-renewal multipotency|
|刊名||JOURNAL OF SURGICAL ONCOLOGY|
|WOS标题词||Science & Technology|
|类目[WOS]||Oncology ; Surgery|
|研究领域[WOS]||Oncology ; Surgery|
|关键词[WOS]||CD133 EXPRESSION ; CANCER ; IDENTIFICATION ; PATHOGENESIS ; PRECURSORS ; MARKER ; GENE ; KIT|
Background: Giant cell tumor of bone (GCTB) is an aggressive benign bone tumor with poor prognosis whose neoplastic component is stromal cells (SCs). Tumor stem-like cells (TSCs) have been demonstrated as precursors for tumor genesis and growth. The aim of this study is to identify TSCs in GCTB.
Methods: Stro-1(+) and Stro-1(-) cells were isolated by fluorescence-activated cell sorting (FACS). Stem-like properties of both Stro-1(+) and Stro-1(-) subpopulations were assessed using MTT colorimetric assays, cell cycle analyses, sphere formation assays, and differentiation assays. Molecular profiles were analyzed by flow cytometry, immunofluorescence, and qRT-PCR.
Results: The existence of rare Stro-1(+) cells was confirmed in vitro using FACS and in vivo by immunohistochemistry. These Stro-1(+) cells exhibited higher proliferative and cisplatin-resistant potentials than Stro-1(-) cells. In serum-free suspension cultures, Stro-1(+) SCs could form cell spheres and maintain self-renewal. Furthermore, Stro-1(+) SCs could differentiate into two mesenchymal lineage cells: osteoblasts and adipocytes. Cell surface markers CD44, CD117, and CD133 and stem cell-associated genes OCT3/4, NANOG, and ABCG2 were significantly higher in the Stro-1(+) subpopulation. Conclusions: This study demonstrates that Stro-1(+) SCs in GCTB possess stem-like biological and molecular phenotypes, indicating that they are the TSCs of GCTB. J. Surg. Oncol. 2012; 106:826836. (C) 2012 Wiley Periodicals, Inc.
|作者单位||1.Peking Univ, Hosp 3, Dept Orthopaed, Beijing 100191, Peoples R China|
2.Peking Univ, Hosp 3, Stem Cell Res Ctr, Beijing 100191, Peoples R China
|Lan, Jie,Liu, Xiaoguang,Rong, Wei,et al. Stro-1(+) stromal cells have stem-like features in giant cell tumor of bone[J]. JOURNAL OF SURGICAL ONCOLOGY,2012,106(7):826-836.|
|APA||Lan, Jie.,Liu, Xiaoguang.,Rong, Wei.,Wei, Feng.,Jiang, Liang.,...&Liu, Zhongjun.(2012).Stro-1(+) stromal cells have stem-like features in giant cell tumor of bone.JOURNAL OF SURGICAL ONCOLOGY,106(7),826-836.|
|MLA||Lan, Jie,et al."Stro-1(+) stromal cells have stem-like features in giant cell tumor of bone".JOURNAL OF SURGICAL ONCOLOGY 106.7(2012):826-836.|