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学科主题: 临床医学
题名:
Stro-1(+) stromal cells have stem-like features in giant cell tumor of bone
作者: Lan, Jie1,2; Liu, Xiaoguang1; Rong, Wei1,2; Wei, Feng1; Jiang, Liang1; Yu, Haiyan2; Dang, Gengting1; Liu, Zhongjun1
关键词: giant cell tumor of bone ; neoplastic stem cells ; Stro-1 ; self-renewal ; multipotency
刊名: JOURNAL OF SURGICAL ONCOLOGY
发表日期: 2012-12-01
DOI: 10.1002/jso.23151
卷: 106, 期:7, 页:826-836
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Surgery
研究领域[WOS]: Oncology ; Surgery
关键词[WOS]: CD133 EXPRESSION ; CANCER ; IDENTIFICATION ; PATHOGENESIS ; PRECURSORS ; MARKER ; GENE ; KIT
英文摘要:

Background: Giant cell tumor of bone (GCTB) is an aggressive benign bone tumor with poor prognosis whose neoplastic component is stromal cells (SCs). Tumor stem-like cells (TSCs) have been demonstrated as precursors for tumor genesis and growth. The aim of this study is to identify TSCs in GCTB.

Methods: Stro-1(+) and Stro-1(-) cells were isolated by fluorescence-activated cell sorting (FACS). Stem-like properties of both Stro-1(+) and Stro-1(-) subpopulations were assessed using MTT colorimetric assays, cell cycle analyses, sphere formation assays, and differentiation assays. Molecular profiles were analyzed by flow cytometry, immunofluorescence, and qRT-PCR.

Results: The existence of rare Stro-1(+) cells was confirmed in vitro using FACS and in vivo by immunohistochemistry. These Stro-1(+) cells exhibited higher proliferative and cisplatin-resistant potentials than Stro-1(-) cells. In serum-free suspension cultures, Stro-1(+) SCs could form cell spheres and maintain self-renewal. Furthermore, Stro-1(+) SCs could differentiate into two mesenchymal lineage cells: osteoblasts and adipocytes. Cell surface markers CD44, CD117, and CD133 and stem cell-associated genes OCT3/4, NANOG, and ABCG2 were significantly higher in the Stro-1(+) subpopulation. Conclusions: This study demonstrates that Stro-1(+) SCs in GCTB possess stem-like biological and molecular phenotypes, indicating that they are the TSCs of GCTB. J. Surg. Oncol. 2012; 106:826836. (C) 2012 Wiley Periodicals, Inc.

语种: 英语
WOS记录号: WOS:000310541900006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60573
Appears in Collections:北京大学第三临床医学院_骨科_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Dept Orthopaed, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Stem Cell Res Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Lan, Jie,Liu, Xiaoguang,Rong, Wei,et al. Stro-1(+) stromal cells have stem-like features in giant cell tumor of bone[J]. JOURNAL OF SURGICAL ONCOLOGY,2012,106(7):826-836.
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