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学科主题: 基础医学
题名:
Allogeneic bone marrow-derived flk-1(+)Sca-(1-) mesenchymal stem cells leads to stable mixed chimerism and donor-specific tolerance
作者: Deng, WM; Han, Q; Liao, LM; Li, CH; Ge, W; Zhao, ZG; You, SG; Deng, HY; Zhao, RCH
刊名: EXPERIMENTAL HEMATOLOGY
发表日期: 2004-09-01
DOI: 10.1016/j.exphem.2004.06.009
卷: 32, 期:9, 页:861-867
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Hematology ; Medicine, Research & Experimental
研究领域[WOS]: Hematology ; Research & Experimental Medicine
关键词[WOS]: STROMAL CELLS ; IN-VITRO ; TRANSPLANTATION ; ENGRAFTMENT ; REJECTION ; BLOOD ; UTERO ; MICE ; VIVO
英文摘要:

Objective. To investigate the possibility of flk-1(+)Sca-1(-) bone marrow-derived mesenchymal stem cells (bMSCs) to induce stable mixed chimerism and donor-specific graft tolerance.

Methods. Allogeneic flk-1(+)Sca-1(-) bMSCs and syngeneic bone marrow (BM) cells were cotransplanted into lethally irradiated (8.5 Gy) recipient mice. FACS was used to analyze the chimerism 150 days later. Donor-type skin transplantation was performed to observe donor-specific immunotolerance in recipient mice. Mixed lymphocyte reaction (MLR) and mitogen proliferative assays were performed to evaluate proliferative response of splenocytes from recipient mice.

Results. More than 5% donor-derived CD3(+) cells were detected in splenocytes of recipient mice. Long-term survival of donor-type skin grafts was observed. MLR and mitogen proliferative assays showed that recipient mice had low immunoresponse to donor cells but retained normal ConA-induced proliferative response compared with normal mice.

Conclusion. Our results show for the first time that induction of stable mixed hematopoietic chimerism can be achieved with allogeneic flk-1(+)Sca-1(-) bMSC transplantation, which leads to permanent donor-specific immunotolerance in allogeneic host and results in long-term allogeneic skin graft acceptance. (C) 2004 International Society for Experimental Hematology. Published by Elsevier Inc.

语种: 英语
WOS记录号: WOS:000224117500010
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60576
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Chinese Acad Med Sci, Inst Basic Med Sci, Sch Basic Sci, Peking Union Med Coll,Ctr Excellence Tissue Engn, Beijing 100730, Peoples R China
2.Chinese Acad Med Sci, Inst Hematol, State Key Lab Expt Hematol, Tianjin, Peoples R China
3.Chinese Acad Med Sci, Blood Dis Hosp, Tianjin, Peoples R China
4.Peking Union Med Coll, Tianjin, Peoples R China
5.Peking Univ, Dept Immunol, Hlth Sci Ctr, Beijing 100871, Peoples R China
6.Peking Union Med Coll, Beijing, Peoples R China

Recommended Citation:
Deng, WM,Han, Q,Liao, LM,et al. Allogeneic bone marrow-derived flk-1(+)Sca-(1-) mesenchymal stem cells leads to stable mixed chimerism and donor-specific tolerance[J]. EXPERIMENTAL HEMATOLOGY,2004,32(9):861-867.
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