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学科主题: 基础医学
题名:
Influence of TRAIL gene on biomechanical properties of the human leukemic cell line Jurkat
作者: Yao, WJ; Chen, K; Wang, XJ; Xie, LD; Wen, ZY; Yan, ZY; Chien, S
关键词: TRAIL ; tet gene expression system ; Jurkat ; apoptosis ; biomechanics
刊名: JOURNAL OF BIOMECHANICS
发表日期: 2002-12-01
卷: 35, 期:12, 页:1659-1663
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biophysics ; Engineering, Biomedical
研究领域[WOS]: Biophysics ; Engineering
关键词[WOS]: TET-ON SYSTEM ; INHIBITION ; EXPRESSION ; INDUCTION ; LIGAND
英文摘要:

We cloned the cDNA fragment of human TNF-related apoptosis inducing ligand (TRAIL) into RevTet-On, a Tet-regulated and high-level gene expression system. Making use of the TRAIL gene expression system in Jurkat as a cell model, we studied the influence of TRAIL gene on the biomechanics properties of Jurkat through measuring changes of cellular biomechanics properties before and after the TRAIL gene expression, which was induced by adding tetracycline derivative doxycycline (Dox). The results indicated that the TRAIL gene expression led to significant changes in cellular biomechanics properties. The osmotic fragility increased and the cell stiffness increased after the expression of TRAIL gene. Thus, the apoptosis-inducing TRAIL gene caused significant changes in the biomechanics properties of Jurkat cells. (C) 2002 Elsevier Science Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000179812900015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60583
Appears in Collections:基础医学院_生物物理学系_期刊论文

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作者单位: 1.Peking Univ, Dept Biophys, Sch Basic Med Sci, Hemorheol Ctr, Beijing 100083, Peoples R China
2.Peking Univ, Dept Biochem & Mol Biol, Sch Basic Med Sci, Beijing 100083, Peoples R China
3.Peking Univ, Dept Mech & Engn Sci, Beijing 100871, Peoples R China
4.Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA
5.Univ Calif San Diego, Whitaker Inst Biomed Engn, La Jolla, CA 92093 USA

Recommended Citation:
Yao, WJ,Chen, K,Wang, XJ,et al. Influence of TRAIL gene on biomechanical properties of the human leukemic cell line Jurkat[J]. JOURNAL OF BIOMECHANICS,2002,35(12):1659-1663.
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