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学科主题: 基础医学
题名:
Ion Chromatography in Combination with ESI Ion Trap MS for the Analysis of Diphacinone in Animal Livers
作者: Cai, Mei-Qiang2; Chen, Xiao-Hong1; Yan, Yong-Qing3; Jin, Mi-Cong1
关键词: Column ion chromatography-mass spectrometry ; Gradient eluent generator ; Diphacinone and rodenticide in animal liver
刊名: CHROMATOGRAPHIA
发表日期: 2009
DOI: 10.1365/s10337-008-0844-7
卷: 69, 期:1-2, 页:33-38
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: IONIZATION MASS-SPECTROMETRY ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; 8 ANTICOAGULANT RODENTICIDES ; MULTIRESIDUE ANALYSIS ; HUMAN PLASMA ; CHLOROPHACINONE ; TISSUES ; RESIDUES ; SERUM ; WATER
英文摘要:

Analysis of diphacinone is often performed on C18 column by reserved phase liquid chromatographic methods coupled with ultraviolet detection or by ion-pair chromatography coupled with mass spectrometry. In this work, a sensitive and selective method using eluent generator reagent free ion chromatography coupled with electrospray ionization ion trap mass spectrometric detection for the quantification of diphacinone in animal livers has been developed. Samples were extracted with 10% (v/v) methanol in acetonitrile after spiking with warfarin (internal standard, IS) and cleaned by solid-phase extraction. The analytes were separated on an IonPac AS11 analytical column (250 mm x 4.0 mm) using 30.0 mmol L-1 KOH (achieved by a KOH eluent generator) containing 10% (v/v) methanol as organic modifier at a flow rate of 1.0 mL min(-1). Detection was performed by negative electrospray ionization and ion trap tandem mass spectrometry in multiple reaction monitoring mode with a transition of m/z 339 -> 167 for diphacinone, and 307 -> 161 for IS, respectively. The calibration curve was linear (r(2) = 0.993) in the concentration range of 1.0-200.0 ng mL(-1) with a lower limit of quantification of 0.3 ng g(-1) in animal livers. Intra- and inter-day relative standard deviations (RSDs) were less than 8.7 and 12.5%, respectively. Recoveries of diphacinone ranged from 81.2 to 89.5%. It was confirmed that this method could be used in clinical diagnosis or forensic toxicology.

语种: 英语
所属项目编号: 2007A610072 ; 2007C10084 ; 2007QN014
项目资助者: Natural Science Foundation of Ningbo, China ; Agriculture and Social Development Funds of Ningbo, China ; Medical Health Youth Funds in Zhejiang Province, China
WOS记录号: WOS:000262967400005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60594
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Peking Univ, Sch Basic Med Sci, Beijing 100083, Peoples R China
2.Ningbo Municipal Ctr Dis Control & Prevent, Ningbo 315010, Zhejiang, Peoples R China
3.Zhejiang Gongshang Univ, Coll Environm Sci & Engn, Hangzhou 310035, Zhejiang, Peoples R China

Recommended Citation:
Cai, Mei-Qiang,Chen, Xiao-Hong,Yan, Yong-Qing,et al. Ion Chromatography in Combination with ESI Ion Trap MS for the Analysis of Diphacinone in Animal Livers[J]. CHROMATOGRAPHIA,2009,69(1-2):33-38.
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