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学科主题基础医学
Ion Chromatography in Combination with ESI Ion Trap MS for the Analysis of Diphacinone in Animal Livers
Cai, Mei-Qiang2; Chen, Xiao-Hong1; Yan, Yong-Qing3; Jin, Mi-Cong1
关键词Column ion chromatography-mass spectrometry Gradient eluent generator Diphacinone and rodenticide in animal liver
刊名CHROMATOGRAPHIA
2009
DOI10.1365/s10337-008-0844-7
69期:1-2页:33-38
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]IONIZATION MASS-SPECTROMETRY ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; 8 ANTICOAGULANT RODENTICIDES ; MULTIRESIDUE ANALYSIS ; HUMAN PLASMA ; CHLOROPHACINONE ; TISSUES ; RESIDUES ; SERUM ; WATER
英文摘要

Analysis of diphacinone is often performed on C18 column by reserved phase liquid chromatographic methods coupled with ultraviolet detection or by ion-pair chromatography coupled with mass spectrometry. In this work, a sensitive and selective method using eluent generator reagent free ion chromatography coupled with electrospray ionization ion trap mass spectrometric detection for the quantification of diphacinone in animal livers has been developed. Samples were extracted with 10% (v/v) methanol in acetonitrile after spiking with warfarin (internal standard, IS) and cleaned by solid-phase extraction. The analytes were separated on an IonPac AS11 analytical column (250 mm x 4.0 mm) using 30.0 mmol L-1 KOH (achieved by a KOH eluent generator) containing 10% (v/v) methanol as organic modifier at a flow rate of 1.0 mL min(-1). Detection was performed by negative electrospray ionization and ion trap tandem mass spectrometry in multiple reaction monitoring mode with a transition of m/z 339 -> 167 for diphacinone, and 307 -> 161 for IS, respectively. The calibration curve was linear (r(2) = 0.993) in the concentration range of 1.0-200.0 ng mL(-1) with a lower limit of quantification of 0.3 ng g(-1) in animal livers. Intra- and inter-day relative standard deviations (RSDs) were less than 8.7 and 12.5%, respectively. Recoveries of diphacinone ranged from 81.2 to 89.5%. It was confirmed that this method could be used in clinical diagnosis or forensic toxicology.

语种英语
WOS记录号WOS:000262967400005
项目编号2007A610072 ; 2007C10084 ; 2007QN014
资助机构Natural Science Foundation of Ningbo, China ; Agriculture and Social Development Funds of Ningbo, China ; Medical Health Youth Funds in Zhejiang Province, China
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60594
专题北京大学基础医学院
作者单位1.Peking Univ, Sch Basic Med Sci, Beijing 100083, Peoples R China
2.Ningbo Municipal Ctr Dis Control & Prevent, Ningbo 315010, Zhejiang, Peoples R China
3.Zhejiang Gongshang Univ, Coll Environm Sci & Engn, Hangzhou 310035, Zhejiang, Peoples R China
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GB/T 7714
Cai, Mei-Qiang,Chen, Xiao-Hong,Yan, Yong-Qing,et al. Ion Chromatography in Combination with ESI Ion Trap MS for the Analysis of Diphacinone in Animal Livers[J]. CHROMATOGRAPHIA,2009,69(1-2):33-38.
APA Cai, Mei-Qiang,Chen, Xiao-Hong,Yan, Yong-Qing,&Jin, Mi-Cong.(2009).Ion Chromatography in Combination with ESI Ion Trap MS for the Analysis of Diphacinone in Animal Livers.CHROMATOGRAPHIA,69(1-2),33-38.
MLA Cai, Mei-Qiang,et al."Ion Chromatography in Combination with ESI Ion Trap MS for the Analysis of Diphacinone in Animal Livers".CHROMATOGRAPHIA 69.1-2(2009):33-38.
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