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学科主题临床医学
Induction of Ro/SSA antigen expression on keratinocyte cell membrane by heat shock and phorbol 12-myristate 13-acetate as well as estradiol and ultraviolet B
Zhang, JH; Xu, ZG; Jin, J; Zhu, TJ; Ma, SQ
关键词Ro/SSA heat shock phorbol ester ultraviolet B
刊名JOURNAL OF DERMATOLOGICAL SCIENCE
2000-11-01
24期:2页:92-98
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dermatology
研究领域[WOS]Dermatology
关键词[WOS]CULTURED HUMAN KERATINOCYTES ; SYSTEMIC LUPUS-ERYTHEMATOSUS ; EXTRACTABLE NUCLEAR ANTIGENS ; CUTANEOUS LUPUS ; POSSIBLE MECHANISM ; SS-A/RO ; SURFACE ; BINDING ; IRRADIATION ; ANTIBODIES
英文摘要

Skin is one of the main target organs in lupus erythematosus and in some circumstances, skin lesions precede systemic manifestations. Previous studies have demonstrated that Ro/SSA antigen antibody might be involved in the pathogenesis of lupus erythematosus. The present study was performed to investigate the factors regulating expression of Ro/SSA antigens on the cell surface of keratinocytes. Cultured normal human keratinocytes were treated with 50-200 mJ/cm(2) of ultraviolet B (UVB) irradiation, 10(-9) to 10(-5) mol/l of 17 beta -estradiol, 5-10 mug/l of phorbol 12-myristate 13-acetate (PMA), and 42 and 45 degreesC heat shock, respectively. The Ro/SSA antigen expressions were determined by indirect immunofluorescence. The results showed that keratinocytes receiving UVB irradiation expressed Ro/SSA antigen on cell membranes in a dose-dependent fashion. 17 beta -estradiol treatment also induced Ro/SSA antigen expression dose-dependently. Keratinocyte expression of Ro/SSA antigens was also induced by heat shock stimulation. The 45 degreesC heat shock showed a stronger effect than 42 degreesC heat shack. Keratinocytes incubated for 24 h after heat shock had more antigen-expressing cells than those incubated for 6 h after heat shock. PMA at 5 and 10 mug/l also strongly induced Ro/SSA antigen expression. These results suggest that Ro/SSA antigen expression can be regulated by many factors and that protein kinase C signal transduction pathway might be involved in this process. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

语种英语
WOS记录号WOS:000165092400003
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60596
专题北京大学第二临床医学院_皮科
作者单位1.Beijing Med Univ, Peoples Hosp, Dept Dermatol, Beijing 100044, Peoples R China
2.Beijing Med Univ, Hosp 1, Dept Dermatol, Beijing 100034, Peoples R China
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Zhang, JH,Xu, ZG,Jin, J,et al. Induction of Ro/SSA antigen expression on keratinocyte cell membrane by heat shock and phorbol 12-myristate 13-acetate as well as estradiol and ultraviolet B[J]. JOURNAL OF DERMATOLOGICAL SCIENCE,2000,24(2):92-98.
APA Zhang, JH,Xu, ZG,Jin, J,Zhu, TJ,&Ma, SQ.(2000).Induction of Ro/SSA antigen expression on keratinocyte cell membrane by heat shock and phorbol 12-myristate 13-acetate as well as estradiol and ultraviolet B.JOURNAL OF DERMATOLOGICAL SCIENCE,24(2),92-98.
MLA Zhang, JH,et al."Induction of Ro/SSA antigen expression on keratinocyte cell membrane by heat shock and phorbol 12-myristate 13-acetate as well as estradiol and ultraviolet B".JOURNAL OF DERMATOLOGICAL SCIENCE 24.2(2000):92-98.
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