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学科主题: 临床医学
题名:
Molecular Characterization and Antimicrobial Susceptibility Testing of Escherichia coli Isolates from Patients with Urinary Tract Infections in 20 Chinese Hospitals
作者: Cao, Xiaoli2; Cavaco, Lina M.1; Lv, Yuan3; Li, Yun3; Zheng, Bo3; Wang, Pengyuan2; Hasman, Henrik1; Liu, Yucun2; Aarestrup, Frank M.1
刊名: JOURNAL OF CLINICAL MICROBIOLOGY
发表日期: 2011-07-01
DOI: 10.1128/JCM.02503-10
卷: 49, 期:7, 页:2496-2501
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
关键词[WOS]: SPECTRUM BETA-LACTAMASES ; MEDIATED QUINOLONE RESISTANCE ; GRAM-NEGATIVE BACTERIA ; EXTENDED-SPECTRUM ; SALMONELLA-ENTERICA ; CTX-M ; PHYLOGENETIC GROUP ; PROTEUS-MIRABILIS ; PLASMIDS ; CTX-M-15
英文摘要:

A total of 222 urinary Escherichia coli isolates from 20 tertiary hospitals in 15 different provinces and 4 municipalities in mainland China were characterized by antimicrobial susceptibility, phylogrouping, and the presence of plasmid-mediated quinolone resistance genes. A subset of 138 suspected extended-spectrum cephalosporinase (ESC) producers were examined for genes encoding cephalosporin resistance. Forty-three isolates harboring bla(CTX-M-14) or bla(CTX-M-15) were analyzed by pulsed-field gel electrophoresis (PFGE), and plasmids containing these genes were typed using PCR-based replicon typing (PBRT). Thirteen phylogroup B2 bla(CTX-M-14)- and bla(CTX-M-15)-positive isolates were analyzed by multilocus sequence typing (MLST). A frequent occurrence of resistance (> 46%) was observed toward cephalosporins, gentamicin, and fluoroquinolones. Among the 222 isolates, 4 qnrS1, 4 qepA, and 16 aac(6′)-Ib-cr genes were confirmed. Four major phylogroups (A, B1, B2, and D) and nontypeable isolates (NTs) were found among the isolates, with phylogroup D (54%) being the most common phylogroup. A total of 110 (80%) of the 138 screened isolates harbored bla(CTX-M) genes, with bla(CTX-M-14) (71%) and bla(CTX-M-15) (24%) being the most prevalent of these genes. Nine of the 13 CTX-M-15-or CTX-M-14-containing B2 isolates belonged to ST131. PFGE typing showed a high level of diversity, and plasmid analysis indicated a very large pool of different resistance plasmids mediating the spread of bla(CTX-M) genes in mainland China. An equally very high frequency of resistance and equally high levels of diversity in phylogroups, PFGE types, and plasmids were observed among community-and hospital-acquired E. coli isolates, indicating the presence of a large reservoir in the community and a long-term spread of cephalosporin resistance in China.

语种: 英语
所属项目编号: 09-067103/DSF ; 2008860003 ; 2008ZX09312-017
项目资助者: WHO Global Food-Borne Infection Network (WHO-GFN) ; Center for Genomic Epidemiology ; China Health and Medical Development Foundation ; National Project of Platform Establishment for Clinical Development of New Antimicrobial Agents ; Overseas Study Program of the China Scholarship Council, Beijing, China
WOS记录号: WOS:000292276200017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60610
Appears in Collections:北京大学第一临床医学院_普通外科_期刊论文

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作者单位: 1.Tech Univ Denmark, Natl Food Inst, WHO Collaborating Ctr Antimicrobial Resistance Fo, DK-2800 Lyngby, Denmark
2.Peking Univ, Hosp 1, Dept Gen Surg, Beijing 100034, Peoples R China
3.Peking Univ, Hosp 1, Inst Clin Pharmacol, Beijing 100034, Peoples R China

Recommended Citation:
Cao, Xiaoli,Cavaco, Lina M.,Lv, Yuan,et al. Molecular Characterization and Antimicrobial Susceptibility Testing of Escherichia coli Isolates from Patients with Urinary Tract Infections in 20 Chinese Hospitals[J]. JOURNAL OF CLINICAL MICROBIOLOGY,2011,49(7):2496-2501.
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