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学科主题基础医学
Sulforaphane Protects Rodent Retinas against Ischemia-Reperfusion Injury through the Activation of the Nrf2/HO-1 Antioxidant Pathway
Pan, Hong1,2,3,4,5; He, Meihua2,4,5; Liu, Ruixing2,4,5; Brecha, Nicholas C.6,7; Yu, Albert Cheung Hoi1; Pu, Mingliang2,4,5
刊名PLOS ONE
2014-12-03
DOI10.1371/journal.pone.0114186
9期:12
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]NRF2-DEPENDENT PHASE-2 ENZYME ; OXIDATIVE STRESS ; HEME OXYGENASE-1 ; ISCHEMIA/REPERFUSION INJURY ; PHARMACOLOGICAL INDUCTION ; CALCIUM DOBESILATE ; RESPONSE ELEMENT ; KAPPA-B ; CELLS ; GENE
英文摘要

Retinal ischemia-reperfusion (I/R) injury induces oxidative stress, leukocyte infiltration, and neuronal cell death. Sulforaphane (SF), which can be obtained in cruciferous vegetables such as broccoli, exerts protective effects in response to oxidative stress in various tissues. These effects can be initiated through nuclear factor E2-related factor 2 (Nrf2)-mediated induction of heme oxygenase-1 (HO-1). This investigation was designed to elucidate the neural protective mechanisms of SF in the retinal I/R rat model. Animals were intraperitoneally (i.p.) injected with SF (12.5 mg/kg) or vehicle (corn oil) once a day for 7 consecutive days. Then, retinal I/R was made by elevating the intraocular pressure (IOP) to 130 mmHg for 1 h. To determine if HO-1 was involved in the Nrf2 antioxidant pathway, rats were subjected to protoporphyrin IX zinc (II) (ZnPP, 30 mg/kg, i.p.) treatments at 24 h before retinal ischemia. The neuroprotective effects of SF were assessed by determining the morphology of the retina, counting the infiltrating inflammatory cells and the surviving retinal ganglion cells (RGCs) and amacrine cells, and measuring apoptosis in the retinal layers. The expression of Nrf2 and HO-1 was studied by immunofluorescence analysis and western blotting. I/R induced a marked increase of ROS generation, caused pronounced inflammation, increased the apoptosis of RGCs and amacrine cells and caused the thinning of the inner retinal layer (IRL), and these effects were diminished or abolished by SF pretreatment. Meanwhile, SF pretreatment significantly elevated the nuclear accumulation of Nrf2 and the level of HO-1 expression in the I/R retinas; however, ZnPP reversed the protective effects of SF on I/R retinas. Together, we offer direct evidence that SF had protective effects on I/R retinas, which could be attributed, at least in part, to the activation of the Nrf2/HO-1 antioxidant pathway.

语种英语
WOS记录号WOS:000349128700082
项目编号2011CB510206 ; 30831160516 ; 81200691 ; 20090001120075 ; 21609101 ; EY04067
资助机构National Basic Research Program of China (973 Program) ; National Science Foundation of China ; Ministry of Education of China ; Azalea Endowment Fund ; Fundamental Research Fund for the Central Universities ; NIH ; VA Merit Review
引用统计
被引频次:25[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60621
专题北京大学基础医学院_神经生物学系
北京大学基础医学院
北京大学第一临床医学院_中心实验室
作者单位1.Peking Univ, Neurosci Res Inst, Dept Neurobiol, Sch Basic Med Sci, Beijing 100871, Peoples R China
2.Peking Univ, Dept Anat Embryol, Sch Basic Med Sci, Beijing 100871, Peoples R China
3.Binzhou Med Coll, Dept Phys, Yantai, Shandong, Peoples R China
4.Peking Univ, Key Lab Machine Percept, Minist Educ, Beijing 100871, Peoples R China
5.Peking Univ, Key Lab Visual Impairment & Restorat, Minist Educ, Beijing 100871, Peoples R China
6.Univ Calif Los Angeles, Dept Med, David Geffen Sch Med Los Angeles, Jules Stein Eye Inst,CURE Digest Dis Res Ctr,Dept, Los Angeles, CA 90024 USA
7.Vet Adm Greater Los Angeles Hlth Syst, Los Angeles, CA USA
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GB/T 7714
Pan, Hong,He, Meihua,Liu, Ruixing,et al. Sulforaphane Protects Rodent Retinas against Ischemia-Reperfusion Injury through the Activation of the Nrf2/HO-1 Antioxidant Pathway[J]. PLOS ONE,2014,9(12).
APA Pan, Hong,He, Meihua,Liu, Ruixing,Brecha, Nicholas C.,Yu, Albert Cheung Hoi,&Pu, Mingliang.(2014).Sulforaphane Protects Rodent Retinas against Ischemia-Reperfusion Injury through the Activation of the Nrf2/HO-1 Antioxidant Pathway.PLOS ONE,9(12).
MLA Pan, Hong,et al."Sulforaphane Protects Rodent Retinas against Ischemia-Reperfusion Injury through the Activation of the Nrf2/HO-1 Antioxidant Pathway".PLOS ONE 9.12(2014).
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