IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
Tissue microarray analysis of multiple gene expression in intestinal metaplasia, dysplasia and carcinoma of the stomach
Sun, Y; Li, JY; He, JS; Zhou, LX; Chen, K
关键词carcinogenesis dysplasia immunohistochemistry intestinal metaplasia microarray stomach
刊名HISTOPATHOLOGY
2005-05-01
DOI10.1111/j.1365-2559.2005.02111.x
46期:5页:505-514
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Pathology
研究领域[WOS]Cell Biology ; Pathology
关键词[WOS]IN-SITU HYBRIDIZATION ; GASTRIC CARCINOGENESIS ; CELL-PROLIFERATION ; MOLECULAR-CLONING ; CYCLIN-E ; CANCER ; P53 ; MUCIN ; AMPLIFICATION ; ADENOCARCINOMAS
英文摘要

Aims: To study multiple gene expression patterns and their roles in the process of gastric carcinogenesis.

Methods and results: Using a high-throughput tissue microarray technique, 169 specimens from gastric carcinomas, precursor lesions and normal mucosa were immunostained on a series of tissue chips for p53, p21(WAF1/CIP1) cyclin E, Bcl-2, c-met and mucin 5AC expression. The overexpression of p53 was observed in 10.7% of low-grade dysplasia (LGD), 38.1% of high-grade dysplasia (HGD) and 39.6% of intestinal type gastric carcinoma (IGC). Expression of p21(WAF1/CIP1) was found in 47.6% of incomplete intestinal metaplasia (IM), 36.7% of dysplasia (Dys) and 29.5% of IGC. The overexpression of cyclin E was more frequently present in carcinomas than in Dys (P < 0.05); moreover, high-level expression (> 25% in extent) of cyclin E was observed only among IGC. Abnormal Bcl-2 expression was present in 81.0% of incomplete IM, 69.4% of Dys and 22.9% of IGC. Along with progression of the lesion, the expression of c-met increased; in contrast, mucin 5AC decreased gradually.

Conclusions: The specific expression pattern in incomplete IM was mucin 5AC(+)/Bcl-2(+)/p53(-)/cyclin E(-), while mucin 5AC(-)/cyclin E(+) was specific for IGC. p53 was useful for distinguishing LGD from HGD. High-level expression of cyclin E might be an indicator for malignant transformation of dysplasia.

语种英语
WOS记录号WOS:000228489400004
引用统计
被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60627
专题北京大学临床肿瘤学院
作者单位Peking Univ, Sch Oncol, Beijing Canc Hosp, Beijing Inst Canc Res,Dept Pathol, Beijing 100871, Peoples R China
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GB/T 7714
Sun, Y,Li, JY,He, JS,et al. Tissue microarray analysis of multiple gene expression in intestinal metaplasia, dysplasia and carcinoma of the stomach[J]. HISTOPATHOLOGY,2005,46(5):505-514.
APA Sun, Y,Li, JY,He, JS,Zhou, LX,&Chen, K.(2005).Tissue microarray analysis of multiple gene expression in intestinal metaplasia, dysplasia and carcinoma of the stomach.HISTOPATHOLOGY,46(5),505-514.
MLA Sun, Y,et al."Tissue microarray analysis of multiple gene expression in intestinal metaplasia, dysplasia and carcinoma of the stomach".HISTOPATHOLOGY 46.5(2005):505-514.
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