|Tissue microarray analysis of multiple gene expression in intestinal metaplasia, dysplasia and carcinoma of the stomach|
|Sun, Y; Li, JY; He, JS; Zhou, LX; Chen, K|
|关键词||carcinogenesis dysplasia immunohistochemistry intestinal metaplasia microarray stomach|
|WOS标题词||Science & Technology|
|类目[WOS]||Cell Biology ; Pathology|
|研究领域[WOS]||Cell Biology ; Pathology|
|关键词[WOS]||IN-SITU HYBRIDIZATION ; GASTRIC CARCINOGENESIS ; CELL-PROLIFERATION ; MOLECULAR-CLONING ; CYCLIN-E ; CANCER ; P53 ; MUCIN ; AMPLIFICATION ; ADENOCARCINOMAS|
Aims: To study multiple gene expression patterns and their roles in the process of gastric carcinogenesis.
Methods and results: Using a high-throughput tissue microarray technique, 169 specimens from gastric carcinomas, precursor lesions and normal mucosa were immunostained on a series of tissue chips for p53, p21(WAF1/CIP1) cyclin E, Bcl-2, c-met and mucin 5AC expression. The overexpression of p53 was observed in 10.7% of low-grade dysplasia (LGD), 38.1% of high-grade dysplasia (HGD) and 39.6% of intestinal type gastric carcinoma (IGC). Expression of p21(WAF1/CIP1) was found in 47.6% of incomplete intestinal metaplasia (IM), 36.7% of dysplasia (Dys) and 29.5% of IGC. The overexpression of cyclin E was more frequently present in carcinomas than in Dys (P < 0.05); moreover, high-level expression (> 25% in extent) of cyclin E was observed only among IGC. Abnormal Bcl-2 expression was present in 81.0% of incomplete IM, 69.4% of Dys and 22.9% of IGC. Along with progression of the lesion, the expression of c-met increased; in contrast, mucin 5AC decreased gradually.
Conclusions: The specific expression pattern in incomplete IM was mucin 5AC(+)/Bcl-2(+)/p53(-)/cyclin E(-), while mucin 5AC(-)/cyclin E(+) was specific for IGC. p53 was useful for distinguishing LGD from HGD. High-level expression of cyclin E might be an indicator for malignant transformation of dysplasia.
|作者单位||Peking Univ, Sch Oncol, Beijing Canc Hosp, Beijing Inst Canc Res,Dept Pathol, Beijing 100871, Peoples R China|
|Sun, Y,Li, JY,He, JS,et al. Tissue microarray analysis of multiple gene expression in intestinal metaplasia, dysplasia and carcinoma of the stomach[J]. HISTOPATHOLOGY,2005,46(5):505-514.|
|APA||Sun, Y,Li, JY,He, JS,Zhou, LX,&Chen, K.(2005).Tissue microarray analysis of multiple gene expression in intestinal metaplasia, dysplasia and carcinoma of the stomach.HISTOPATHOLOGY,46(5),505-514.|
|MLA||Sun, Y,et al."Tissue microarray analysis of multiple gene expression in intestinal metaplasia, dysplasia and carcinoma of the stomach".HISTOPATHOLOGY 46.5(2005):505-514.|