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学科主题: 临床医学
题名:
Efficient purification of GP-1D8 glycoprotein from human breast carcinoma tissue by immunoaffinity chromatography
作者: Feng, Renqing1; Guo, Zhenquan1; Li, Jinfeng2
关键词: breast cancer ; glycoprotein ; immunoaffinity chromatography ; purification
刊名: PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
发表日期: 2008
DOI: 10.1080/10826060802325204
卷: 38, 期:4, 页:321-333
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
研究领域[WOS]: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]: AFFINITY-CHROMATOGRAPHY ; PROTEIN-G ; BIOSEPARATION ; BINDING
英文摘要:

Anti-GP-1D8 monoclonal antibody was produced in our laboratory. Immunoaffinity purification of GP-1D8 glycoprotein from human breast carcinoma tissues, on column with the monospecific antibody, is developed. The procedure permits purification of GP-1D8 to a highly purified state. It appeared as a single band in sodium dodcyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The molecular mass of GP-1D8 was determined to be 66kDa by mass spectrometry. Its antigenicity was stable between 0 and 70 degrees C. It was breast cancer specific, as determined by immunohistochemistry and immunocytochemistry. The preparation of anti-GP-1D8 monoclonal antibody will facilitate further detection of, and functional study on, GP-1D8. The study also provides a simple, economical, and efficient method for affinity purification of target proteins from human or animal tissues.

语种: 英语
所属项目编号: 30571711
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000259314700001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60638
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: 1.Peking Univ, Coll Life Sci, Dept Biochem, Beijing 100871, Peoples R China
2.Peking Univ, Sch Oncol, Dept Surg, Beijing 100871, Peoples R China

Recommended Citation:
Feng, Renqing,Guo, Zhenquan,Li, Jinfeng. Efficient purification of GP-1D8 glycoprotein from human breast carcinoma tissue by immunoaffinity chromatography[J]. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,2008,38(4):321-333.
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