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学科主题临床医学
Efficient purification of GP-1D8 glycoprotein from human breast carcinoma tissue by immunoaffinity chromatography
Feng, Renqing1; Guo, Zhenquan1; Li, Jinfeng2
关键词breast cancer glycoprotein immunoaffinity chromatography purification
刊名PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
2008
DOI10.1080/10826060802325204
38期:4页:321-333
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]AFFINITY-CHROMATOGRAPHY ; PROTEIN-G ; BIOSEPARATION ; BINDING
英文摘要

Anti-GP-1D8 monoclonal antibody was produced in our laboratory. Immunoaffinity purification of GP-1D8 glycoprotein from human breast carcinoma tissues, on column with the monospecific antibody, is developed. The procedure permits purification of GP-1D8 to a highly purified state. It appeared as a single band in sodium dodcyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The molecular mass of GP-1D8 was determined to be 66kDa by mass spectrometry. Its antigenicity was stable between 0 and 70 degrees C. It was breast cancer specific, as determined by immunohistochemistry and immunocytochemistry. The preparation of anti-GP-1D8 monoclonal antibody will facilitate further detection of, and functional study on, GP-1D8. The study also provides a simple, economical, and efficient method for affinity purification of target proteins from human or animal tissues.

语种英语
WOS记录号WOS:000259314700001
项目编号30571711
资助机构National Natural Science Foundation of China
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60638
专题北京大学临床肿瘤学院
北京大学临床肿瘤学院_乳腺癌预防治疗中心
作者单位1.Peking Univ, Coll Life Sci, Dept Biochem, Beijing 100871, Peoples R China
2.Peking Univ, Sch Oncol, Dept Surg, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Feng, Renqing,Guo, Zhenquan,Li, Jinfeng. Efficient purification of GP-1D8 glycoprotein from human breast carcinoma tissue by immunoaffinity chromatography[J]. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,2008,38(4):321-333.
APA Feng, Renqing,Guo, Zhenquan,&Li, Jinfeng.(2008).Efficient purification of GP-1D8 glycoprotein from human breast carcinoma tissue by immunoaffinity chromatography.PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,38(4),321-333.
MLA Feng, Renqing,et al."Efficient purification of GP-1D8 glycoprotein from human breast carcinoma tissue by immunoaffinity chromatography".PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY 38.4(2008):321-333.
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