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学科主题: 临床医学
题名:
YAP is overexpressed in clear cell renal cell carcinoma and its knockdown reduces cell proliferation and induces cell cycle arrest and apoptosis
作者: Cao, Jian-Jia1; Zhao, Xiu-Min1; Wang, De-Lin1; Chen, Ke-Hong1; Sheng, Xia1; Li, Wen-Bin1; Li, Mei-Cai1; Liu, Wu-Jiang2; He, Jiang3
关键词: clear cell renal cell carcinoma ; Yes-associated protein ; TEAD1 ; RNA interference ; lentivirus
刊名: ONCOLOGY REPORTS
发表日期: 2014-10-01
DOI: 10.3892/or.2014.3349
卷: 32, 期:4, 页:1594-1600
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: YES-ASSOCIATED PROTEIN ; MEDIATED RNA INTERFERENCE ; ORGAN SIZE CONTROL ; HIPPO PATHWAY ; CONTACT INHIBITION ; GROWTH-CONTROL ; CANCER ; TARGET ; TRANSCRIPTION ; EXPRESSION
英文摘要:

Yes-associated protein (YAP) has been reported to be an oncogene in a number of malignancies. It constitutes an important regulatory mechanism for the Hippo pathway, a key regulator of cell growth and apoptosis. The present study aimed to investigate the clinical significance and the role of YAP in the development of clear cell renal cell carcinoma (ccRCC). YAP expression levels were compared between ccRCC and adjacent normal renal tissues by RT-PCR and immunohistochemistry, respectively. YAP expression levels were then detected in ccRCC cell lines 786-0 and ACHN, as well as in human embryonic kidney 293 cells (HEK-293) using western blotting. Three specific YAP-shRNA lentiviral vectors were constructed and transfected into 786-0 cells, and then the mRNA and protein levels of YAP and downstream transcription factor TEAD1 were detected. Finally, the effects of YAP silencing on proliferation and the cell cycle distribution of 786-0 cells were detected by Cell Counting Kit-8 (CCK-8) and flow cytometry (FCM), respectively. The apoptosis rate was also analyzed by FCM. It was observed that the expression levels of YAP mRNA and protein in ccRCC tissues were higher than these levels in the adjacent normal renal tissues. The expression of YAP protein in ccRCC tissues was significantly correlated with clinical stage and differentiation. The YAP protein levels in the two ccRCC cell lines 786-0 and ACHN were significantly higher than that in the HEK-293 cells. Additionally, treatment of 786-0 cells with YAP-shRNA lentiviral vectors significantly reduced the expression levels of YAP and TEAD1 mRNA and protein. Further analyses in 786-0 cells in which YAP was decreased, revealed that cell proliferation was inhibited, cell cycle was arrested at the G1 phase and apoptosis was increased. These results indicate that YAP is an underlying oncogene in ccRCC and it may be a promising biomarker and therapeutic target of ccRCC.

语种: 英语
所属项目编号: 30972999 ; cqpc2012jja1698
项目资助者: Natural Science Foundation of China ; Nature Science Foundation of Chongqing
WOS记录号: WOS:000342528200037
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60641
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

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作者单位: 1.Chongqing Med Univ, Affiliated Hosp 1, Dept Urol, Chongqing 400016, Peoples R China
2.Peking Univ, Hosp 1, Inst Urol, Beijing 100871, Peoples R China
3.Chongqing Med Univ, Univ Town Hosp, Gastroenterol & Neurol Ctr, Chongqing 400016, Peoples R China

Recommended Citation:
Cao, Jian-Jia,Zhao, Xiu-Min,Wang, De-Lin,et al. YAP is overexpressed in clear cell renal cell carcinoma and its knockdown reduces cell proliferation and induces cell cycle arrest and apoptosis[J]. ONCOLOGY REPORTS,2014,32(4):1594-1600.
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