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学科主题临床医学
RNA interference-mediated targeting of DKK1 gene expression in Ishikawa endometrial carcinoma cells causes increased tumor cell invasion and migration
Yi, Nuo1; Liao, Qin-Ping2; Li, Zhen-Hua1; Xie, Bao-Jiang3; Hu, Yu-Hong1; Yi, Wei1; Liu, Min1
关键词beta-catenin DKK1 endometrial carcinoma invasion migration MMP14 RNAi
刊名ONCOLOGY LETTERS
2013-09-01
DOI10.3892/ol.2013.1439
6期:3页:756-762
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]WNT SIGNALING PATHWAY ; TROPHOBLAST INVASION ; CANCER METASTASIS ; DICKKOPF-1 ; ACTIVATION ; MECHANISM ; RECEPTOR
英文摘要

The Wnt signaling pathway plays an essential role in tumor invasion and migration. DKK1 functions as an important inhibitor of the pathway and represents a promising target for cancer therapy. The aim of the present study was to determine the role of DKK1 in endometrial carcinoma (EC) cell invasion and migration using RNA interference (RNAi) technology. Ishikawa EC cells were transfected at high efficiency with specific DKK1 siRNA. RT-PCR and western blot analysis were used to determine the mRNA and protein levels of DKK1, beta-catenin and metalloproteinase 14 (MMP14) in siRNA-treated and -untreated cells. In addition, the invasion and migration of the EC cells were detected by invasion and migration assays. Transient transfection of DKK1 siRNA significantly inhibited the mRNA and protein levels of DKK1. Markedly increased cell invasion and migration was observed following treatment with DKK1 siRNA when compared with the negative control siRNA-treated and siRNA-untreated cells. The knockdown of DKK1 also elevated the mRNA and protein levels of beta-catenin and MMP14 involved in the Wnt signaling pathway, indicating that targeting this gene may promote intracellular Wnt signal transduction and thus, accelerate EC cell invasion and migration in vitro. The RNAi-mediated targeting of DKK1 gene expression in Ishikawa EC cells resulted in increased tumor cell invasion and migration. DKK1 was identified as an inhibitor of EC cell invasion and migration via its novel role in the Wnt signaling pathway. Targeting DKK1 may therefore represent an effective anti-invasion and -migration strategy for the treatment of EC.

语种英语
WOS记录号WOS:000322382700025
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60650
专题北京大学第一临床医学院
作者单位1.Capital Med Univ, Beijing Ditan Hosp, Dept Obstet & Gynecol, Beijing 100015, Peoples R China
2.Peking Univ, Hosp 1, Dept Obstet & Gynecol, Beijing 100034, Peoples R China
3.Beijing Int Studies Univ Hosp, Dept Surg, Beijing 100024, Peoples R China
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Yi, Nuo,Liao, Qin-Ping,Li, Zhen-Hua,et al. RNA interference-mediated targeting of DKK1 gene expression in Ishikawa endometrial carcinoma cells causes increased tumor cell invasion and migration[J]. ONCOLOGY LETTERS,2013,6(3):756-762.
APA Yi, Nuo.,Liao, Qin-Ping.,Li, Zhen-Hua.,Xie, Bao-Jiang.,Hu, Yu-Hong.,...&Liu, Min.(2013).RNA interference-mediated targeting of DKK1 gene expression in Ishikawa endometrial carcinoma cells causes increased tumor cell invasion and migration.ONCOLOGY LETTERS,6(3),756-762.
MLA Yi, Nuo,et al."RNA interference-mediated targeting of DKK1 gene expression in Ishikawa endometrial carcinoma cells causes increased tumor cell invasion and migration".ONCOLOGY LETTERS 6.3(2013):756-762.
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