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学科主题: 临床医学
题名:
RNA interference-mediated targeting of DKK1 gene expression in Ishikawa endometrial carcinoma cells causes increased tumor cell invasion and migration
作者: Yi, Nuo1; Liao, Qin-Ping2; Li, Zhen-Hua1; Xie, Bao-Jiang3; Hu, Yu-Hong1; Yi, Wei1; Liu, Min1
关键词: beta-catenin ; DKK1 ; endometrial carcinoma ; invasion ; migration ; MMP14 ; RNAi
刊名: ONCOLOGY LETTERS
发表日期: 2013-09-01
DOI: 10.3892/ol.2013.1439
卷: 6, 期:3, 页:756-762
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: WNT SIGNALING PATHWAY ; TROPHOBLAST INVASION ; CANCER METASTASIS ; DICKKOPF-1 ; ACTIVATION ; MECHANISM ; RECEPTOR
英文摘要:

The Wnt signaling pathway plays an essential role in tumor invasion and migration. DKK1 functions as an important inhibitor of the pathway and represents a promising target for cancer therapy. The aim of the present study was to determine the role of DKK1 in endometrial carcinoma (EC) cell invasion and migration using RNA interference (RNAi) technology. Ishikawa EC cells were transfected at high efficiency with specific DKK1 siRNA. RT-PCR and western blot analysis were used to determine the mRNA and protein levels of DKK1, beta-catenin and metalloproteinase 14 (MMP14) in siRNA-treated and -untreated cells. In addition, the invasion and migration of the EC cells were detected by invasion and migration assays. Transient transfection of DKK1 siRNA significantly inhibited the mRNA and protein levels of DKK1. Markedly increased cell invasion and migration was observed following treatment with DKK1 siRNA when compared with the negative control siRNA-treated and siRNA-untreated cells. The knockdown of DKK1 also elevated the mRNA and protein levels of beta-catenin and MMP14 involved in the Wnt signaling pathway, indicating that targeting this gene may promote intracellular Wnt signal transduction and thus, accelerate EC cell invasion and migration in vitro. The RNAi-mediated targeting of DKK1 gene expression in Ishikawa EC cells resulted in increased tumor cell invasion and migration. DKK1 was identified as an inhibitor of EC cell invasion and migration via its novel role in the Wnt signaling pathway. Targeting DKK1 may therefore represent an effective anti-invasion and -migration strategy for the treatment of EC.

语种: 英语
WOS记录号: WOS:000322382700025
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60650
Appears in Collections:北京大学第一临床医学院_期刊论文

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作者单位: 1.Capital Med Univ, Beijing Ditan Hosp, Dept Obstet & Gynecol, Beijing 100015, Peoples R China
2.Peking Univ, Hosp 1, Dept Obstet & Gynecol, Beijing 100034, Peoples R China
3.Beijing Int Studies Univ Hosp, Dept Surg, Beijing 100024, Peoples R China

Recommended Citation:
Yi, Nuo,Liao, Qin-Ping,Li, Zhen-Hua,et al. RNA interference-mediated targeting of DKK1 gene expression in Ishikawa endometrial carcinoma cells causes increased tumor cell invasion and migration[J]. ONCOLOGY LETTERS,2013,6(3):756-762.
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