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学科主题口腔医学
PLGA/PDLLA core-shell submicron spheres sequential release system: Preparation, characterization and promotion of bone regeneration in vitro and in vivo
Wang, Ying1,2; Wei, Yan1; Zhang, Xuehui5; Xu, Mingming1; Liu, Feng2; Ma, Qi1; Cai, Qing3,4; Deng, Xuliang1,6,7
关键词Sequential release Core-shell submicron spheres Bone regeneration BMP-2 VEGF
刊名CHEMICAL ENGINEERING JOURNAL
2015-08-01
DOI10.1016/j.cej.2015.03.068
273页:490-501
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Engineering, Environmental ; Engineering, Chemical
研究领域[WOS]Engineering
关键词[WOS]GROWTH-FACTOR ; DEFECT MODEL ; DELIVERY ; ANGIOGENESIS ; VEGF ; OSTEOGENESIS ; BMP-2 ; NANOPARTICLES ; ELECTROSPRAY ; MICROSPHERES
英文摘要

Core-shell polymer spheres of submicron diameter are a promising vehicle for sequential delivery of angiogenic and osteogenic growth factors to bone defect sites, to simulate the orchestrated temporal sequence of angiogenesis and osteogenesis. To achieve a homogeneous distribution pattern in the scaffold matrix and avoid fast biological clearance in vivo, attention should be paid to the particle size of the spheres, using a modified coaxial electrospraying technique, we prepared core-shell spheres about 1 gm in diameter using the polymers poly-(D,L-lactide) in the shell and poly(L-lactide-co-glycolic acid) in the core, and loaded them with VEGF and BMP-2, growth factors that stimulate angiogenesis and osteogenesis, respectively. PLGA/PDLLA controlled sequential delivery profiles, including an initial burst release of VEGF and a sustained release behavior of BMP-2 from the VEGF//BMP-2 spheres, were obtained. The VEGF and BMP-2 released from the spheres maintained their bioactivity; VEGF could enhance the proliferation of endothelial cells and BMP-2 could promote the osteogenic differentiation of bone marrow mesenchymal stem cells. Micro-computed tomography analysis showed that, among all the experimental groups, implantation of VEGFIIBMP-2 spheres into rat cranial critical-sized bone defects enhanced new bone formation to the greatest extent, resulting in the largest amount of new bone volume and the largest isolated bone islands. Histological examination showed that VEGF//BMP-2 spheres also significantly increased in-growth of blood vessels with positive CD31 staining. All these findings suggest that the submicron-scale core-shell VEGF//BMP-2 spheres developed in this study are capable of yielding sequentially coupled angiogenesis and osteogenesis, implying their extensive application in bone tissue regeneration. (C) 2015 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000354582800057
项目编号2012CB933900 ; 81171000 ; 51302005 ; 7144256 ; 7144257 ; Z14111000180000
资助机构National Basic Research Program of China ; National Natural Science Foundation of China ; Beijing Natural Science Foundation ; Beijing Nova Program
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60657
专题北京大学口腔医学院
北京大学第一临床医学院_超声诊断中心
北京大学口腔医学院_特诊科
北京大学临床肿瘤学院_乳腺癌预防治疗中心
作者单位1.Peking Univ, Sch & Hosp Stomatol, Dept Geriatr Dent, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Clin Div 1, Beijing 100081, Peoples R China
3.Beijing Univ Chem Technol, Beijing Lab Biomed Mat, Beijing 100029, Peoples R China
4.Beijing Univ Chem Technol, State Key Lab Organ Inorgan Composites, Beijing 100029, Peoples R China
5.Peking Univ, Sch & Hosp Stomatol, Dept Dent Mat, Beijing 100081, Peoples R China
6.Natl Engn Lab Digital & Mat Technol Stomatol, Beijing 100081, Peoples R China
7.Peking Univ, Sch & Hosp Stomatol, Dept Prosthodont, Beijing 100081, Peoples R China
推荐引用方式
GB/T 7714
Wang, Ying,Wei, Yan,Zhang, Xuehui,et al. PLGA/PDLLA core-shell submicron spheres sequential release system: Preparation, characterization and promotion of bone regeneration in vitro and in vivo[J]. CHEMICAL ENGINEERING JOURNAL,2015,273:490-501.
APA Wang, Ying.,Wei, Yan.,Zhang, Xuehui.,Xu, Mingming.,Liu, Feng.,...&Deng, Xuliang.(2015).PLGA/PDLLA core-shell submicron spheres sequential release system: Preparation, characterization and promotion of bone regeneration in vitro and in vivo.CHEMICAL ENGINEERING JOURNAL,273,490-501.
MLA Wang, Ying,et al."PLGA/PDLLA core-shell submicron spheres sequential release system: Preparation, characterization and promotion of bone regeneration in vitro and in vivo".CHEMICAL ENGINEERING JOURNAL 273(2015):490-501.
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