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学科主题: 口腔医学
题名:
PLGA/PDLLA core-shell submicron spheres sequential release system: Preparation, characterization and promotion of bone regeneration in vitro and in vivo
作者: Wang, Ying1,2; Wei, Yan1; Zhang, Xuehui5; Xu, Mingming1; Liu, Feng2; Ma, Qi1; Cai, Qing3,4; Deng, Xuliang1,6,7
关键词: Sequential release ; Core-shell submicron spheres ; Bone regeneration ; BMP-2 ; VEGF
刊名: CHEMICAL ENGINEERING JOURNAL
发表日期: 2015-08-01
DOI: 10.1016/j.cej.2015.03.068
卷: 273, 页:490-501
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Engineering, Environmental ; Engineering, Chemical
研究领域[WOS]: Engineering
关键词[WOS]: GROWTH-FACTOR ; DEFECT MODEL ; DELIVERY ; ANGIOGENESIS ; VEGF ; OSTEOGENESIS ; BMP-2 ; NANOPARTICLES ; ELECTROSPRAY ; MICROSPHERES
英文摘要:

Core-shell polymer spheres of submicron diameter are a promising vehicle for sequential delivery of angiogenic and osteogenic growth factors to bone defect sites, to simulate the orchestrated temporal sequence of angiogenesis and osteogenesis. To achieve a homogeneous distribution pattern in the scaffold matrix and avoid fast biological clearance in vivo, attention should be paid to the particle size of the spheres, using a modified coaxial electrospraying technique, we prepared core-shell spheres about 1 gm in diameter using the polymers poly-(D,L-lactide) in the shell and poly(L-lactide-co-glycolic acid) in the core, and loaded them with VEGF and BMP-2, growth factors that stimulate angiogenesis and osteogenesis, respectively. PLGA/PDLLA controlled sequential delivery profiles, including an initial burst release of VEGF and a sustained release behavior of BMP-2 from the VEGF//BMP-2 spheres, were obtained. The VEGF and BMP-2 released from the spheres maintained their bioactivity; VEGF could enhance the proliferation of endothelial cells and BMP-2 could promote the osteogenic differentiation of bone marrow mesenchymal stem cells. Micro-computed tomography analysis showed that, among all the experimental groups, implantation of VEGFIIBMP-2 spheres into rat cranial critical-sized bone defects enhanced new bone formation to the greatest extent, resulting in the largest amount of new bone volume and the largest isolated bone islands. Histological examination showed that VEGF//BMP-2 spheres also significantly increased in-growth of blood vessels with positive CD31 staining. All these findings suggest that the submicron-scale core-shell VEGF//BMP-2 spheres developed in this study are capable of yielding sequentially coupled angiogenesis and osteogenesis, implying their extensive application in bone tissue regeneration. (C) 2015 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 2012CB933900 ; 81171000 ; 51302005 ; 7144256 ; 7144257 ; Z14111000180000
项目资助者: National Basic Research Program of China ; National Natural Science Foundation of China ; Beijing Natural Science Foundation ; Beijing Nova Program
WOS记录号: WOS:000354582800057
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60657
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Dept Geriatr Dent, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Clin Div 1, Beijing 100081, Peoples R China
3.Beijing Univ Chem Technol, Beijing Lab Biomed Mat, Beijing 100029, Peoples R China
4.Beijing Univ Chem Technol, State Key Lab Organ Inorgan Composites, Beijing 100029, Peoples R China
5.Peking Univ, Sch & Hosp Stomatol, Dept Dent Mat, Beijing 100081, Peoples R China
6.Natl Engn Lab Digital & Mat Technol Stomatol, Beijing 100081, Peoples R China
7.Peking Univ, Sch & Hosp Stomatol, Dept Prosthodont, Beijing 100081, Peoples R China

Recommended Citation:
Wang, Ying,Wei, Yan,Zhang, Xuehui,et al. PLGA/PDLLA core-shell submicron spheres sequential release system: Preparation, characterization and promotion of bone regeneration in vitro and in vivo[J]. CHEMICAL ENGINEERING JOURNAL,2015,273:490-501.
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