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学科主题: 临床医学
题名:
Dendritic cell-ewing′s sarcoma cell hybrids enhance antitumor immunity
作者: Guo, Wei1; Guo, Yi2; Tang, Shun1; Qu, Huayi1; Zhao, Hui1
刊名: CLINICAL ORTHOPAEDICS AND RELATED RESEARCH
发表日期: 2008-09-01
DOI: 10.1007/s11999-008-0348-7
卷: 466, 期:9, 页:2176-2183
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Orthopedics ; Surgery
研究领域[WOS]: Orthopedics ; Surgery
关键词[WOS]: CYTOTOXIC T-LYMPHOCYTES ; POLYETHYLENE-GLYCOL ; HUMAN TUMOR ; IN-VITRO ; FUSION ; ELECTROFUSION ; ANTIGEN ; FAMILY ; CANCER ; ACTIVATION
英文摘要:

Given the effective immunotherapy of DC-based vaccine in other cancers, we hypothesized DC-based vaccines would induce effective immune responses against Ewing′s sarcoma. To verify this hypothesis and develop the most effective dendritic cell vaccine against Ewing′s sarcoma, we evaluated the antitumor efficacy of dendritic cell-Ewing′s sarcoma hybrids and dendritic cells pulsed with other antigen-loading methods, including cell lysates and the characteristic EWS-FLI1 gene of Ewing′s sarcoma, using an A673 cell line as a model. The hybrids were generated by electrofusion with fusion efficiency and viability determined by flow cytometry and fluorescent microscopy analyses. By interferon-gamma secretion assay, the capacity of hybrids to stimulate cytotoxic T-lymphocytes (CTLs) is higher than that of other antigen-loading methods showing stronger tumor antigen-specific CTL cytotoxicity to A673. By in vivo experiment in SCID mice, all dendritic cell-based strategies induced specific immune responses to Ewing′s sarcoma after mice-human immune system reconstitution by inoculating human peripheral blood mononuclear cells into the peritoneal cavity of SCID mice. However, the hybrids most inhibited the subcutaneous tumor growth in SCID mice compared with dendritic cells pulsed with other loading methods. The data suggest A673 cells respond to dendritic cell-based immunotherapy.

语种: 英语
WOS记录号: WOS:000258152700017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60665
Appears in Collections:北京大学第二临床医学院_骨肿瘤科_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Musculoskeletal Tumor Ctr, Beijing 100044, Peoples R China
2.Univ Calif Irvine, Med Ctr, Dept Orthopaed, Irvine, CA USA

Recommended Citation:
Guo, Wei,Guo, Yi,Tang, Shun,et al. Dendritic cell-ewing′s sarcoma cell hybrids enhance antitumor immunity[J]. CLINICAL ORTHOPAEDICS AND RELATED RESEARCH,2008,466(9):2176-2183.
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