IR@PKUHSC  > 北京大学第二临床医学院  > 骨肿瘤科
学科主题临床医学
Dendritic cell-ewing′s sarcoma cell hybrids enhance antitumor immunity
Guo, Wei1; Guo, Yi2; Tang, Shun1; Qu, Huayi1; Zhao, Hui1
刊名CLINICAL ORTHOPAEDICS AND RELATED RESEARCH
2008-09-01
DOI10.1007/s11999-008-0348-7
466期:9页:2176-2183
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Orthopedics ; Surgery
研究领域[WOS]Orthopedics ; Surgery
关键词[WOS]CYTOTOXIC T-LYMPHOCYTES ; POLYETHYLENE-GLYCOL ; HUMAN TUMOR ; IN-VITRO ; FUSION ; ELECTROFUSION ; ANTIGEN ; FAMILY ; CANCER ; ACTIVATION
英文摘要

Given the effective immunotherapy of DC-based vaccine in other cancers, we hypothesized DC-based vaccines would induce effective immune responses against Ewing′s sarcoma. To verify this hypothesis and develop the most effective dendritic cell vaccine against Ewing′s sarcoma, we evaluated the antitumor efficacy of dendritic cell-Ewing′s sarcoma hybrids and dendritic cells pulsed with other antigen-loading methods, including cell lysates and the characteristic EWS-FLI1 gene of Ewing′s sarcoma, using an A673 cell line as a model. The hybrids were generated by electrofusion with fusion efficiency and viability determined by flow cytometry and fluorescent microscopy analyses. By interferon-gamma secretion assay, the capacity of hybrids to stimulate cytotoxic T-lymphocytes (CTLs) is higher than that of other antigen-loading methods showing stronger tumor antigen-specific CTL cytotoxicity to A673. By in vivo experiment in SCID mice, all dendritic cell-based strategies induced specific immune responses to Ewing′s sarcoma after mice-human immune system reconstitution by inoculating human peripheral blood mononuclear cells into the peritoneal cavity of SCID mice. However, the hybrids most inhibited the subcutaneous tumor growth in SCID mice compared with dendritic cells pulsed with other loading methods. The data suggest A673 cells respond to dendritic cell-based immunotherapy.

语种英语
WOS记录号WOS:000258152700017
引用统计
被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60665
专题北京大学第二临床医学院_骨肿瘤科
北京大学药学院_天然药物与仿生药物国家重点实验室
作者单位1.Peking Univ, Peoples Hosp, Musculoskeletal Tumor Ctr, Beijing 100044, Peoples R China
2.Univ Calif Irvine, Med Ctr, Dept Orthopaed, Irvine, CA USA
推荐引用方式
GB/T 7714
Guo, Wei,Guo, Yi,Tang, Shun,et al. Dendritic cell-ewing′s sarcoma cell hybrids enhance antitumor immunity[J]. CLINICAL ORTHOPAEDICS AND RELATED RESEARCH,2008,466(9):2176-2183.
APA Guo, Wei,Guo, Yi,Tang, Shun,Qu, Huayi,&Zhao, Hui.(2008).Dendritic cell-ewing′s sarcoma cell hybrids enhance antitumor immunity.CLINICAL ORTHOPAEDICS AND RELATED RESEARCH,466(9),2176-2183.
MLA Guo, Wei,et al."Dendritic cell-ewing′s sarcoma cell hybrids enhance antitumor immunity".CLINICAL ORTHOPAEDICS AND RELATED RESEARCH 466.9(2008):2176-2183.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Guo, Wei]的文章
[Guo, Yi]的文章
[Tang, Shun]的文章
百度学术
百度学术中相似的文章
[Guo, Wei]的文章
[Guo, Yi]的文章
[Tang, Shun]的文章
必应学术
必应学术中相似的文章
[Guo, Wei]的文章
[Guo, Yi]的文章
[Tang, Shun]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。