IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
Influences of amino acid sequences in FR1 region on binding activity of the scFv and Fab of an antibody to human gastric cancer cells
Li, J; Wang, Y; Wang, ZZ; Dong, ZW
关键词human gastric cancer scFv Fab PCR
刊名IMMUNOLOGY LETTERS
2000-03-01
71期:3页:157-165
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology
研究领域[WOS]Immunology
关键词[WOS]SINGLE-CHAIN FV ; ESCHERICHIA-COLI ; VARIABLE DOMAINS ; MONOCLONAL-ANTIBODIES ; SPECIFICITY ; AFFINITY ; EXPRESSION ; REPERTOIRE ; SECRETION ; RESIDUES
英文摘要

Murine anti-gastric cancer mAb 3H11 has promising clinical applications. In this work, engineering of 3H11 scFv and Fab was conducted to increase its usefulness. 3H11 scFv and Fab were constructed by PCR amplification of the V-domains with degenerate primers for FR1. The bacterially expressed 3H11 Ab fragments showed no antigen binding activity. Then the N-terminal sequences of V regions were mutated to the 3H11 original sequence. The expressed scFv and Fab in bacterial culture supernatant showed binding activity to gastric cancer cells. Comparing the expression of unmutated and mutated 3H11 Fab, we found that the sequence changes of the V region N terminus introduced by PCR may seriously affect antigen binding but not the expression of antibody. Correction of either VL or VH N-terminal sequences can partially restore the antigen binding activity (61% for VL and 73% for VH). (C) 7000 Elsevier Science B.V. All rights reserved.

语种英语
WOS记录号WOS:000086175500004
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60673
专题北京大学临床肿瘤学院
作者单位1.Beijing Med Univ, Sch Oncol, Beijing 100034, Peoples R China
2.Navy Gen Hosp, Beijing 100037, Peoples R China
3.Chinese Acad Med Sci, Inst Canc Res, Beijing 100021, Peoples R China
推荐引用方式
GB/T 7714
Li, J,Wang, Y,Wang, ZZ,et al. Influences of amino acid sequences in FR1 region on binding activity of the scFv and Fab of an antibody to human gastric cancer cells[J]. IMMUNOLOGY LETTERS,2000,71(3):157-165.
APA Li, J,Wang, Y,Wang, ZZ,&Dong, ZW.(2000).Influences of amino acid sequences in FR1 region on binding activity of the scFv and Fab of an antibody to human gastric cancer cells.IMMUNOLOGY LETTERS,71(3),157-165.
MLA Li, J,et al."Influences of amino acid sequences in FR1 region on binding activity of the scFv and Fab of an antibody to human gastric cancer cells".IMMUNOLOGY LETTERS 71.3(2000):157-165.
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