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学科主题: 临床医学
题名:
Establishment and characterization of patient-derived tumor xenograft using gastroscopic biopsies in gastric cancer
作者: Zhu, Yan1; Tian, Tiantian1; Li, Zhongwu2; Tang, Zhiyu3; Wang, Lai3; Wu, Jian4; Li, Yilin1; Dong, Bin2; Li, Yanyan1; Li, Na1; Zou, Jianling1; Gao, Jing1; Shen, Lin1
刊名: SCIENTIFIC REPORTS
发表日期: 2015-02-25
DOI: 10.1038/srep08542
卷: 5
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: COLORECTAL-CANCER ; LUNG CANCERS ; MOUSE MODELS ; NUDE-MICE ; COMBINATION ; CARCINOMAS ; GROWTH ; AMPLIFICATION ; CHEMOTHERAPY ; TRASTUZUMAB
英文摘要:

The patient-derived tumor xenograft (PDTX) model has become the most realistic model for preclinical studies. PDTX models of gastric cancer using surgical tissues are reported occasionally; however, the PDTX models using gastroscopic biopsies, which are best for evaluating new drugs, are unreported. In our study, a total of 185 fresh gastroscopic biopsies of gastric cancer were subcutaneously transplanted into NOD/SCID (Nonobese Diabetic/Severe Combined Immunodeficiency) mice. Sixty-three PDTX models were successfully established (34.1%, 63/185) and passaged to maintain tumors in vivo, and the mean latency period of xenografts was 65.86 +/- 32.84 days (11-160 days). Biopsies of prior chemotherapy had a higher transplantation rate (52.1%, 37/71) than biopsies after chemotherapy (21.9%, 25/114; P = 0.000). No differences were found between the latency period of xenografts and characteristics of patients. The pathological and molecular features of PDTX as well as chemosensitivity were highly consistent with those of primary tumors of patients. The genetic characteristics were stable during passaging of PDTX models. In summary PDTX models using gastroscopic biopsies in gastric cancer were demonstrated for the first time, and the biological characteristics of the PDTX models were highly consistent with patients, which provided the best preclinical study platform for gastric cancer.

语种: 英语
所属项目编号: Z11110706730000 ; 81172110 ; 2012AA 02A 504
项目资助者: Beijing Municipal Science &amp ; Technology Commission Program ; National Natural Science Foundation of China ; National High Technology Research and Development Program
WOS记录号: WOS:000349894400002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60686
Appears in Collections:北京大学临床肿瘤学院_胃肠肿瘤中心_期刊论文

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作者单位: 1.BeiGene Beijing Co Ltd, Beijing, Peoples R China
2.MyGenostics Inc, Beijing, Peoples R China
3.Peking Univ, Canc Hosp & Inst, Dept Gastrointestinal Oncol, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100871, Peoples R China
4.Peking Univ, Canc Hosp & Inst, Dept Pathol, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100871, Peoples R China

Recommended Citation:
Zhu, Yan,Tian, Tiantian,Li, Zhongwu,et al. Establishment and characterization of patient-derived tumor xenograft using gastroscopic biopsies in gastric cancer[J]. SCIENTIFIC REPORTS,2015,5.
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