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学科主题临床医学
Establishment and characterization of patient-derived tumor xenograft using gastroscopic biopsies in gastric cancer
Zhu, Yan1; Tian, Tiantian1; Li, Zhongwu2; Tang, Zhiyu3; Wang, Lai3; Wu, Jian4; Li, Yilin1; Dong, Bin2; Li, Yanyan1; Li, Na1; Zou, Jianling1; Gao, Jing1; Shen, Lin1
刊名SCIENTIFIC REPORTS
2015-02-25
DOI10.1038/srep08542
5
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者Beijing Municipal Science &amp ; Technology Commission Program ; National Natural Science Foundation of China ; National High Technology Research and Development Program ; Beijing Municipal Science &amp ; Technology Commission Program ; National Natural Science Foundation of China ; National High Technology Research and Development Program
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]COLORECTAL-CANCER ; LUNG CANCERS ; MOUSE MODELS ; NUDE-MICE ; COMBINATION ; CARCINOMAS ; GROWTH ; AMPLIFICATION ; CHEMOTHERAPY ; TRASTUZUMAB
英文摘要

The patient-derived tumor xenograft (PDTX) model has become the most realistic model for preclinical studies. PDTX models of gastric cancer using surgical tissues are reported occasionally; however, the PDTX models using gastroscopic biopsies, which are best for evaluating new drugs, are unreported. In our study, a total of 185 fresh gastroscopic biopsies of gastric cancer were subcutaneously transplanted into NOD/SCID (Nonobese Diabetic/Severe Combined Immunodeficiency) mice. Sixty-three PDTX models were successfully established (34.1%, 63/185) and passaged to maintain tumors in vivo, and the mean latency period of xenografts was 65.86 +/- 32.84 days (11-160 days). Biopsies of prior chemotherapy had a higher transplantation rate (52.1%, 37/71) than biopsies after chemotherapy (21.9%, 25/114; P = 0.000). No differences were found between the latency period of xenografts and characteristics of patients. The pathological and molecular features of PDTX as well as chemosensitivity were highly consistent with those of primary tumors of patients. The genetic characteristics were stable during passaging of PDTX models. In summary PDTX models using gastroscopic biopsies in gastric cancer were demonstrated for the first time, and the biological characteristics of the PDTX models were highly consistent with patients, which provided the best preclinical study platform for gastric cancer.

语种英语
所属项目编号Z11110706730000 ; 81172110 ; 2012AA 02A 504
资助者Beijing Municipal Science &amp ; Technology Commission Program ; National Natural Science Foundation of China ; National High Technology Research and Development Program ; Beijing Municipal Science &amp ; Technology Commission Program ; National Natural Science Foundation of China ; National High Technology Research and Development Program
WOS记录号WOS:000349894400002
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60686
专题北京大学临床肿瘤学院_胃肠肿瘤中心
作者单位1.BeiGene Beijing Co Ltd, Beijing, Peoples R China
2.MyGenostics Inc, Beijing, Peoples R China
3.Peking Univ, Canc Hosp & Inst, Dept Gastrointestinal Oncol, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100871, Peoples R China
4.Peking Univ, Canc Hosp & Inst, Dept Pathol, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Zhu, Yan,Tian, Tiantian,Li, Zhongwu,et al. Establishment and characterization of patient-derived tumor xenograft using gastroscopic biopsies in gastric cancer[J]. SCIENTIFIC REPORTS,2015,5.
APA Zhu, Yan.,Tian, Tiantian.,Li, Zhongwu.,Tang, Zhiyu.,Wang, Lai.,...&Shen, Lin.(2015).Establishment and characterization of patient-derived tumor xenograft using gastroscopic biopsies in gastric cancer.SCIENTIFIC REPORTS,5.
MLA Zhu, Yan,et al."Establishment and characterization of patient-derived tumor xenograft using gastroscopic biopsies in gastric cancer".SCIENTIFIC REPORTS 5(2015).
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