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学科主题: 公共卫生
题名:
Claspin is involved in S-phase checkpoint induced by benzo(a)pyrene in 16HBE cells
作者: Qi, Yanmin; Zhao, Peng; Fu, Juanling; Yao, Biyun; Yuan, Zhun; Hu, Entan; Zhou, Zongcan
关键词: Benzo(a)pyrene (BaP) ; Claspin ; Cell cycle ; S-phase checkpoint
刊名: TOXICOLOGY IN VITRO
发表日期: 2009-08-01
DOI: 10.1016/j.tiv.2009.05.006
卷: 23, 期:5, 页:880-886
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Toxicology
研究领域[WOS]: Toxicology
关键词[WOS]: DNA-DAMAGE CHECKPOINTS ; CYCLE CHECKPOINT ; MCF-7 CELLS ; CHK1 ; REPLICATION ; PROTEIN ; ATR ; CDC25A ; DEGRADATION ; ACTIVATION
英文摘要:

Environmental carcinogen benzo(a)pyrene (BaP) can damage DNA by forming bulky adducts that are degraded further to DNA strand breaks, thus contributing to induce DNA damage checkpoint response. Claspin is a critical checkpoint protein in response to multiple forms of genotoxic stress including UV, IR and hydroxyurea (HU). In the present study we have investigated the role of human Claspin in the DNA damage checkpoint elicited by BaP in 16HBE cells. We observed that Claspin levels are increased in a time-dependent manner in response to S-phase arrest induced by BaP. In addition, the levels of phosphorylation of Chk1 on S345 were increased, but the levels of Cdc25A were decreased after treatment with BaP. Inhibition of Claspin expression (siRNA) attenuated the effect of BaP on S-phase arrest and abrogated the activation of Chk1 and degradation of Cdc25A in response to BaP. Taken together, these data imply that Claspin plays an important role in S-phase checkpoint induced by Ball, and it participates in the activation of Chk1 and Cdc25A in this checkpoint pathway. (C) 2009 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 2002CB12906
项目资助者: China National Key Basic Research and Development Program
WOS记录号: WOS:000268345500016
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60702
Appears in Collections:北京大学公共卫生学院_毒理学系_期刊论文

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作者单位: Peking Univ, Hlth Sci Ctr, Dept Toxicol, Beijing 100083, Peoples R China

Recommended Citation:
Qi, Yanmin,Zhao, Peng,Fu, Juanling,et al. Claspin is involved in S-phase checkpoint induced by benzo(a)pyrene in 16HBE cells[J]. TOXICOLOGY IN VITRO,2009,23(5):880-886.
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