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Claspin is involved in S-phase checkpoint induced by benzo(a)pyrene in 16HBE cells
Qi, Yanmin; Zhao, Peng; Fu, Juanling; Yao, Biyun; Yuan, Zhun; Hu, Entan; Zhou, Zongcan
关键词Benzo(a)pyrene (BaP) Claspin Cell cycle S-phase checkpoint
刊名TOXICOLOGY IN VITRO
2009-08-01
DOI10.1016/j.tiv.2009.05.006
23期:5页:880-886
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Toxicology
研究领域[WOS]Toxicology
关键词[WOS]DNA-DAMAGE CHECKPOINTS ; CYCLE CHECKPOINT ; MCF-7 CELLS ; CHK1 ; REPLICATION ; PROTEIN ; ATR ; CDC25A ; DEGRADATION ; ACTIVATION
英文摘要

Environmental carcinogen benzo(a)pyrene (BaP) can damage DNA by forming bulky adducts that are degraded further to DNA strand breaks, thus contributing to induce DNA damage checkpoint response. Claspin is a critical checkpoint protein in response to multiple forms of genotoxic stress including UV, IR and hydroxyurea (HU). In the present study we have investigated the role of human Claspin in the DNA damage checkpoint elicited by BaP in 16HBE cells. We observed that Claspin levels are increased in a time-dependent manner in response to S-phase arrest induced by BaP. In addition, the levels of phosphorylation of Chk1 on S345 were increased, but the levels of Cdc25A were decreased after treatment with BaP. Inhibition of Claspin expression (siRNA) attenuated the effect of BaP on S-phase arrest and abrogated the activation of Chk1 and degradation of Cdc25A in response to BaP. Taken together, these data imply that Claspin plays an important role in S-phase checkpoint induced by Ball, and it participates in the activation of Chk1 and Cdc25A in this checkpoint pathway. (C) 2009 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000268345500016
项目编号2002CB12906
资助机构China National Key Basic Research and Development Program
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被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60702
专题北京大学公共卫生学院_毒理学系
北京大学公共卫生学院_公共卫生学院
作者单位Peking Univ, Hlth Sci Ctr, Dept Toxicol, Beijing 100083, Peoples R China
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Qi, Yanmin,Zhao, Peng,Fu, Juanling,et al. Claspin is involved in S-phase checkpoint induced by benzo(a)pyrene in 16HBE cells[J]. TOXICOLOGY IN VITRO,2009,23(5):880-886.
APA Qi, Yanmin.,Zhao, Peng.,Fu, Juanling.,Yao, Biyun.,Yuan, Zhun.,...&Zhou, Zongcan.(2009).Claspin is involved in S-phase checkpoint induced by benzo(a)pyrene in 16HBE cells.TOXICOLOGY IN VITRO,23(5),880-886.
MLA Qi, Yanmin,et al."Claspin is involved in S-phase checkpoint induced by benzo(a)pyrene in 16HBE cells".TOXICOLOGY IN VITRO 23.5(2009):880-886.
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