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学科主题: 临床医学
题名:
Construction and characterization of a recombinant fowlpox virus containing HIV-1 multi-epitope-p24 chimeric gene in mice
作者: Zhang LiShu; Jin NingYi; Song YingJin; Wang Hong; Ma HeWen; Li ZiJian; Jiang WenZheng
关键词: HIV-1 ; multi-epitope ; recombinant ; fowlpox virus ; immune response
刊名: SCIENCE IN CHINA SERIES C-LIFE SCIENCES
发表日期: 2007-04-01
DOI: 10.1007/s11427-007-0017-1
卷: 50, 期:2, 页:212-220
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biology
研究领域[WOS]: Life Sciences & Biomedicine - Other Topics
关键词[WOS]: IMMUNE-RESPONSES ; TYPE-1 INFECTION ; VIRAL LOAD ; VACCINE ; PROTECTION ; PROTEIN ; IMMUNIZATION ; INDUCTION ; CHICKENS ; REGIMENS
英文摘要:

The epidemic of HIV/AIDS is sweeping across the world. It is of great importance to figure out new ways to curb this disease. Epitope-based vaccine is one of these solutions. In this study, a chimeric gene was obtained by combination of a designed HIV-1 multi-epitope gene (MEG) and HIV-1 p24 gene. A recombinant plasmid pUTA2-MEGp24 was then constructed by inserting MEGp24 gene into the downstream of the promoter (ATI-P7.5x20) of fowlpox virus (FPV) transfer vector pUTA2. The recombinant plasmid and wild-type FPV 282E4 strain were then co-transfected into CEF cells and homologous recombination occurred. A recombinant virus expressing HIV-1 protein MEGp24 was screened by genome PCR and Western blot assay. Large scale preparation and purification of the recombinant fowlpox virus (rFPV) were then carried out. BALB/c mice were immunized intramuscularly with the rFPV for three times on day 0, 14 and 42. Mice were executed and sampled one week after the third inoculation. Anti-HIV-1 antibody in serum and Th1 cytokines in the supernatant of cultured spleen cells were assayed by ELISA. The count of T lymphocyte subsets and the CTL activity of spleen lymphocytes were analyzed by flow cytometry and lactate dehydrogenase (LDH) release assay, respectively. The results showed that HIV-1 specific antibody in serum and increased T lymphocyte subsets (CD4(+) T, CD8(+) T) were detected in the immunization group. CTL target-killing activity and higher secretion of Th1 cytokines (IFN-gamma and IL-2) of spleen lymphocytes stimulated by H-2(d)-restricted CTL peptide were observed in immunized mice. We concluded that the rFPV may induce HIV-1 specific immunity especially cellular immunity in mice.

语种: 英语
WOS记录号: WOS:000246273700010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60707
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

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作者单位: 1.Acad Mil Med Sci, Inst 11, Changchun 130062, Peoples R China
2.Tianjin Univ, Coll Agr & Biol Engineer, Tianjin 300072, Peoples R China
3.Jinan Univ, Coll Life Sci, Guangzhou 510632, Peoples R China
4.Oklahoma State Univ, Dept Biochem & Mol Biol, Stillwater, OK 74078 USA
5.Peking Univ, Affiliated Hosp 3, Inst Vasc Med, Beijing 100083, Peoples R China
6.E China Normal Univ, Sch Life Sci, Shanghai 200026, Peoples R China

Recommended Citation:
Zhang LiShu,Jin NingYi,Song YingJin,et al. Construction and characterization of a recombinant fowlpox virus containing HIV-1 multi-epitope-p24 chimeric gene in mice[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2007,50(2):212-220.
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