IR@PKUHSC  > 北京大学第三临床医学院  > 心血管内科
学科主题临床医学
Construction and characterization of a recombinant fowlpox virus containing HIV-1 multi-epitope-p24 chimeric gene in mice
Zhang LiShu; Jin NingYi; Song YingJin; Wang Hong; Ma HeWen; Li ZiJian; Jiang WenZheng
关键词HIV-1 multi-epitope recombinant fowlpox virus immune response
刊名SCIENCE IN CHINA SERIES C-LIFE SCIENCES
2007-04-01
DOI10.1007/s11427-007-0017-1
50期:2页:212-220
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biology
研究领域[WOS]Life Sciences & Biomedicine - Other Topics
关键词[WOS]IMMUNE-RESPONSES ; TYPE-1 INFECTION ; VIRAL LOAD ; VACCINE ; PROTECTION ; PROTEIN ; IMMUNIZATION ; INDUCTION ; CHICKENS ; REGIMENS
英文摘要

The epidemic of HIV/AIDS is sweeping across the world. It is of great importance to figure out new ways to curb this disease. Epitope-based vaccine is one of these solutions. In this study, a chimeric gene was obtained by combination of a designed HIV-1 multi-epitope gene (MEG) and HIV-1 p24 gene. A recombinant plasmid pUTA2-MEGp24 was then constructed by inserting MEGp24 gene into the downstream of the promoter (ATI-P7.5x20) of fowlpox virus (FPV) transfer vector pUTA2. The recombinant plasmid and wild-type FPV 282E4 strain were then co-transfected into CEF cells and homologous recombination occurred. A recombinant virus expressing HIV-1 protein MEGp24 was screened by genome PCR and Western blot assay. Large scale preparation and purification of the recombinant fowlpox virus (rFPV) were then carried out. BALB/c mice were immunized intramuscularly with the rFPV for three times on day 0, 14 and 42. Mice were executed and sampled one week after the third inoculation. Anti-HIV-1 antibody in serum and Th1 cytokines in the supernatant of cultured spleen cells were assayed by ELISA. The count of T lymphocyte subsets and the CTL activity of spleen lymphocytes were analyzed by flow cytometry and lactate dehydrogenase (LDH) release assay, respectively. The results showed that HIV-1 specific antibody in serum and increased T lymphocyte subsets (CD4(+) T, CD8(+) T) were detected in the immunization group. CTL target-killing activity and higher secretion of Th1 cytokines (IFN-gamma and IL-2) of spleen lymphocytes stimulated by H-2(d)-restricted CTL peptide were observed in immunized mice. We concluded that the rFPV may induce HIV-1 specific immunity especially cellular immunity in mice.

语种英语
WOS记录号WOS:000246273700010
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60707
专题北京大学第三临床医学院_心血管内科
作者单位1.Acad Mil Med Sci, Inst 11, Changchun 130062, Peoples R China
2.Tianjin Univ, Coll Agr & Biol Engineer, Tianjin 300072, Peoples R China
3.Jinan Univ, Coll Life Sci, Guangzhou 510632, Peoples R China
4.Oklahoma State Univ, Dept Biochem & Mol Biol, Stillwater, OK 74078 USA
5.Peking Univ, Affiliated Hosp 3, Inst Vasc Med, Beijing 100083, Peoples R China
6.E China Normal Univ, Sch Life Sci, Shanghai 200026, Peoples R China
推荐引用方式
GB/T 7714
Zhang LiShu,Jin NingYi,Song YingJin,et al. Construction and characterization of a recombinant fowlpox virus containing HIV-1 multi-epitope-p24 chimeric gene in mice[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2007,50(2):212-220.
APA Zhang LiShu.,Jin NingYi.,Song YingJin.,Wang Hong.,Ma HeWen.,...&Jiang WenZheng.(2007).Construction and characterization of a recombinant fowlpox virus containing HIV-1 multi-epitope-p24 chimeric gene in mice.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,50(2),212-220.
MLA Zhang LiShu,et al."Construction and characterization of a recombinant fowlpox virus containing HIV-1 multi-epitope-p24 chimeric gene in mice".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 50.2(2007):212-220.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhang LiShu]的文章
[Jin NingYi]的文章
[Song YingJin]的文章
百度学术
百度学术中相似的文章
[Zhang LiShu]的文章
[Jin NingYi]的文章
[Song YingJin]的文章
必应学术
必应学术中相似的文章
[Zhang LiShu]的文章
[Jin NingYi]的文章
[Song YingJin]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。