IR@PKUHSC  > 北京大学药学院  > 化学生物学系
学科主题药学
Inhibitory effect of Epimedium extract on S-adenosyl-L-homocysteine hydrolase and biomethylation
Zhang, XY; Li, YJ; Yang, XD; Wang, K; Ni, JZ; Qu, XG
关键词Epimedium extract S-adenosyl-L-homocysteine hydrolase biomethylation
刊名LIFE SCIENCES
2005-11-26
DOI10.1016/j.lfs.2005.04.057
78期:2页:180-186
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]ADENOSYLHOMOCYSTEINE HYDROLASE ; TRANSPORT ACTIVITY ; HUMAN-FIBROBLASTS ; ADENOSYLMETHIONINE ; TRANSMETHYLATIONS ; INDUCTION ; DISEASE ; CYSTINE ; BINDING ; FOLATE
英文摘要

In the present paper, the inhibitory effect of Epimedium extract on the activity of S-adenosyl-L-homocysteine (AdoHcy) Hydrolase was studied. The results showed that Epimedium extract inhibited the activity of recombinant human AdoHcy hydrolase in a dose-dependent manner. This inhibitory effect was also observed in hepatic cell line 7701 and hepatoma HepG2, however, the effect in 7701 cells was more potent than in HepG2 cells. The extract could significantly reduce AdoMet/AdoHcy ratio in 7701 cells in a dose-dependent manner, suggesting reduced biomethylation level in 7701 cells. In contrast, it resulted in elevated AdoMet/AdoHcy ratio in the HepG2 cells. The result of MALDI-MS assay indicated that epimedin A and ikarisoside F from the extract could bind to AdoHcy hydrolase. The present data suggested that Epimedium extract could inhibit the activity of AdoHcy hydrolase, thus regulating the cellular biomethylation as well as reducing cellular Hcy level. These results will provide new clues to the mechanisms of Epimedium in curing of cardiovascular disease and regulating tumor cell growth. (c) 2005 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000233187400009
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60711
Collection北京大学药学院_化学生物学系
作者单位1.Chinese Acad Sci, Grad Sch, Changchun Inst Appl Chem, Changchun 130022, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100083, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Natl Res Labs Nat & Biomimet Drugs, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Zhang, XY,Li, YJ,Yang, XD,et al. Inhibitory effect of Epimedium extract on S-adenosyl-L-homocysteine hydrolase and biomethylation[J]. LIFE SCIENCES,2005,78(2):180-186.
APA Zhang, XY,Li, YJ,Yang, XD,Wang, K,Ni, JZ,&Qu, XG.(2005).Inhibitory effect of Epimedium extract on S-adenosyl-L-homocysteine hydrolase and biomethylation.LIFE SCIENCES,78(2),180-186.
MLA Zhang, XY,et al."Inhibitory effect of Epimedium extract on S-adenosyl-L-homocysteine hydrolase and biomethylation".LIFE SCIENCES 78.2(2005):180-186.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
谷歌学术
谷歌学术Similar articles in
[Zhang, XY]'s Articles
[Li, YJ]'s Articles
[Yang, XD]'s Articles
百度学术
百度学术Similar articles in
[Zhang, XY]'s Articles
[Li, YJ]'s Articles
[Yang, XD]'s Articles
必应学术
必应学术Similar articles in
[Zhang, XY]'s Articles
[Li, YJ]'s Articles
[Yang, XD]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.