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学科主题: 药学
题名:
Inhibitory effect of Epimedium extract on S-adenosyl-L-homocysteine hydrolase and biomethylation
作者: Zhang, XY; Li, YJ; Yang, XD; Wang, K; Ni, JZ; Qu, XG
关键词: Epimedium extract ; S-adenosyl-L-homocysteine hydrolase ; biomethylation
刊名: LIFE SCIENCES
发表日期: 2005-11-26
DOI: 10.1016/j.lfs.2005.04.057
卷: 78, 期:2, 页:180-186
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]: Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]: ADENOSYLHOMOCYSTEINE HYDROLASE ; TRANSPORT ACTIVITY ; HUMAN-FIBROBLASTS ; ADENOSYLMETHIONINE ; TRANSMETHYLATIONS ; INDUCTION ; DISEASE ; CYSTINE ; BINDING ; FOLATE
英文摘要:

In the present paper, the inhibitory effect of Epimedium extract on the activity of S-adenosyl-L-homocysteine (AdoHcy) Hydrolase was studied. The results showed that Epimedium extract inhibited the activity of recombinant human AdoHcy hydrolase in a dose-dependent manner. This inhibitory effect was also observed in hepatic cell line 7701 and hepatoma HepG2, however, the effect in 7701 cells was more potent than in HepG2 cells. The extract could significantly reduce AdoMet/AdoHcy ratio in 7701 cells in a dose-dependent manner, suggesting reduced biomethylation level in 7701 cells. In contrast, it resulted in elevated AdoMet/AdoHcy ratio in the HepG2 cells. The result of MALDI-MS assay indicated that epimedin A and ikarisoside F from the extract could bind to AdoHcy hydrolase. The present data suggested that Epimedium extract could inhibit the activity of AdoHcy hydrolase, thus regulating the cellular biomethylation as well as reducing cellular Hcy level. These results will provide new clues to the mechanisms of Epimedium in curing of cardiovascular disease and regulating tumor cell growth. (c) 2005 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000233187400009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60711
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

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作者单位: 1.Chinese Acad Sci, Grad Sch, Changchun Inst Appl Chem, Changchun 130022, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100083, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Natl Res Labs Nat & Biomimet Drugs, Beijing 100083, Peoples R China

Recommended Citation:
Zhang, XY,Li, YJ,Yang, XD,et al. Inhibitory effect of Epimedium extract on S-adenosyl-L-homocysteine hydrolase and biomethylation[J]. LIFE SCIENCES,2005,78(2):180-186.
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