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Molecular characterization of vancomycin-resistant Enterococcus spp. clinical isolates recovered from hospitalized patients among several medical institutions in China
Sun, Hongli2; Wang, Hui3; Xu, Yingchun2; Jones, Ronald N.1; Costello, Andrew J.1; Liu, Yali2; Li, Gang4; Chen, Minjun2; Mendes, Rodrigo E.1
关键词Glycopeptides VRE CC17
刊名DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
2012-12-01
DOI10.1016/j.diagmicrobio.2012.09.006
74期:4页:399-403
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Infectious Diseases ; Microbiology
研究领域[WOS]Infectious Diseases ; Microbiology
关键词[WOS]GLYCOPEPTIDE RESISTANCE ; VANA GENOTYPE ; TEICOPLANIN RESISTANCE ; VIRULENCE FACTORS ; SURFACE PROTEIN ; MULTIPLEX PCR ; FAECIUM ; GENE ; FAECALIS ; SEQUENCE
英文摘要

The epidemiology and molecular characteristics of vancomycin-resistant enterococci (VRE) from China deserve further investigation. This study reports on the molecular characterization of 101 unique VRE (96 E. faecium and 5 E. faecalis strains) recovered from diverse samples of 12 hospitals in China. MIC results were obtained by reference broth microdilution methods, and vancomycin resistance and virulence genes were screened by polymerase chain reaction. All strains were subjected to pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing. E. faecalis exhibited vancomycin and teicoplanin MIC results at >= 256 mu g/mL and harbored vanA, except for 1 vanB-carrying strain (MIC, 32 and 1 mu g/mL, respectively). This strain had a unique PFGE pattern and was associated with ST410 (clonal complex [CC]4). E. faecium displayed vancomycin MIC values of >= 256 mu g/mL with variable results for teicoplanin (1-256 mu g/mL). One E. faecium had a teicoplanin MIC value of 1 mu g/mL and carried a vanB, while the other 2 strains had teicoplanin MIC values of 4 and 8 mu g/mL and harbored vanA. E. faecalis strains were susceptible to ampicillin, and all VRE displayed a susceptible phenotype to daptomycin, linezolid, and tigecycline. Four E. faecalis from a particular hospital were grouped within a single PFGE type and were associated with ST470 and ST471 (CC4), which are double- and triple-locus variants of ST410 and ST4, respectively. Overall, E. faecium displayed genetic variability, but clonal dissemination was noted within and among hospitals. All E. faecium belonged to STs associated with CC17, except for 1 strain (ST362; CC362). A total of 77.2% and 29.7% of all strains carried esp and hyl, respectively. In conclusion, these results show that vanA-carrying isolates predominated in strains from China, and E. faecium strains are usually associated with a common and human-adapted lineage (CC17). Unlike the majority of clinical E. faecalis (CC2 and CC9). strains included in this study showed ST profiles similar to ST4 (CC4), which has been associated with human infections in other Asia-Pacific countries. (C) 2012 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000311596300014
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60712
专题北京大学第二临床医学院
作者单位1.JMI Labs, Beaver Kreek Ctr 345, N Liberty, IA 52317 USA
2.Peking Univ, Peoples Hosp, Beijing 100871, Peoples R China
3.Chinese Acad Med Sci, Peking Union Med Coll Hosp, Beijing 100730, Peoples R China
4.Ningxia Med Univ, Gen Hosp, Yinchuan, Ningxia, Peoples R China
推荐引用方式
GB/T 7714
Sun, Hongli,Wang, Hui,Xu, Yingchun,et al. Molecular characterization of vancomycin-resistant Enterococcus spp. clinical isolates recovered from hospitalized patients among several medical institutions in China[J]. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE,2012,74(4):399-403.
APA Sun, Hongli.,Wang, Hui.,Xu, Yingchun.,Jones, Ronald N..,Costello, Andrew J..,...&Mendes, Rodrigo E..(2012).Molecular characterization of vancomycin-resistant Enterococcus spp. clinical isolates recovered from hospitalized patients among several medical institutions in China.DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE,74(4),399-403.
MLA Sun, Hongli,et al."Molecular characterization of vancomycin-resistant Enterococcus spp. clinical isolates recovered from hospitalized patients among several medical institutions in China".DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE 74.4(2012):399-403.
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