北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第二临床医学院  > 期刊论文
学科主题: 临床医学
题名:
Molecular characterization of vancomycin-resistant Enterococcus spp. clinical isolates recovered from hospitalized patients among several medical institutions in China
作者: Sun, Hongli2; Wang, Hui3; Xu, Yingchun2; Jones, Ronald N.1; Costello, Andrew J.1; Liu, Yali2; Li, Gang4; Chen, Minjun2; Mendes, Rodrigo E.1
关键词: Glycopeptides ; VRE ; CC17
刊名: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
发表日期: 2012-12-01
DOI: 10.1016/j.diagmicrobio.2012.09.006
卷: 74, 期:4, 页:399-403
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Infectious Diseases ; Microbiology
研究领域[WOS]: Infectious Diseases ; Microbiology
关键词[WOS]: GLYCOPEPTIDE RESISTANCE ; VANA GENOTYPE ; TEICOPLANIN RESISTANCE ; VIRULENCE FACTORS ; SURFACE PROTEIN ; MULTIPLEX PCR ; FAECIUM ; GENE ; FAECALIS ; SEQUENCE
英文摘要:

The epidemiology and molecular characteristics of vancomycin-resistant enterococci (VRE) from China deserve further investigation. This study reports on the molecular characterization of 101 unique VRE (96 E. faecium and 5 E. faecalis strains) recovered from diverse samples of 12 hospitals in China. MIC results were obtained by reference broth microdilution methods, and vancomycin resistance and virulence genes were screened by polymerase chain reaction. All strains were subjected to pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing. E. faecalis exhibited vancomycin and teicoplanin MIC results at >= 256 mu g/mL and harbored vanA, except for 1 vanB-carrying strain (MIC, 32 and 1 mu g/mL, respectively). This strain had a unique PFGE pattern and was associated with ST410 (clonal complex [CC]4). E. faecium displayed vancomycin MIC values of >= 256 mu g/mL with variable results for teicoplanin (1-256 mu g/mL). One E. faecium had a teicoplanin MIC value of 1 mu g/mL and carried a vanB, while the other 2 strains had teicoplanin MIC values of 4 and 8 mu g/mL and harbored vanA. E. faecalis strains were susceptible to ampicillin, and all VRE displayed a susceptible phenotype to daptomycin, linezolid, and tigecycline. Four E. faecalis from a particular hospital were grouped within a single PFGE type and were associated with ST470 and ST471 (CC4), which are double- and triple-locus variants of ST410 and ST4, respectively. Overall, E. faecium displayed genetic variability, but clonal dissemination was noted within and among hospitals. All E. faecium belonged to STs associated with CC17, except for 1 strain (ST362; CC362). A total of 77.2% and 29.7% of all strains carried esp and hyl, respectively. In conclusion, these results show that vanA-carrying isolates predominated in strains from China, and E. faecium strains are usually associated with a common and human-adapted lineage (CC17). Unlike the majority of clinical E. faecalis (CC2 and CC9). strains included in this study showed ST profiles similar to ST4 (CC4), which has been associated with human infections in other Asia-Pacific countries. (C) 2012 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000311596300014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60712
Appears in Collections:北京大学第二临床医学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.JMI Labs, Beaver Kreek Ctr 345, N Liberty, IA 52317 USA
2.Peking Univ, Peoples Hosp, Beijing 100871, Peoples R China
3.Chinese Acad Med Sci, Peking Union Med Coll Hosp, Beijing 100730, Peoples R China
4.Ningxia Med Univ, Gen Hosp, Yinchuan, Ningxia, Peoples R China

Recommended Citation:
Sun, Hongli,Wang, Hui,Xu, Yingchun,et al. Molecular characterization of vancomycin-resistant Enterococcus spp. clinical isolates recovered from hospitalized patients among several medical institutions in China[J]. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE,2012,74(4):399-403.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Sun, Hongli]'s Articles
[Wang, Hui]'s Articles
[Xu, Yingchun]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Sun, Hongli]‘s Articles
[Wang, Hui]‘s Articles
[Xu, Yingchun]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2018  北京大学医学部 - Feedback
Powered by CSpace