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Inhibitory role of GDP on saikosaponin (I) stimulated enzymes secretion and rising of [Ca2+](i) in rat pancreatic acini
Yang, WX; Yu, Y; Zhang, WZ; Wang, H; Li, XD; Zhao, YY; Liang, H
关键词saikosaponin ( I ) guanosine diphosphate pancreas cell membrane permeability secretion calcium
刊名ACTA PHARMACOLOGICA SINICA
2001-07-01
22期:7页:669-672
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]CELLS
英文摘要

AIM: To study the role of guanosine diphosphate (GDP) on enzyme secretion and rising of [Ca2+](i) in saikosaponin (I) [SA(I)I stimulated rat pancreatic acini. METHODS: Cell membrane of isolated rat pancreatic acini were permeabilized using streptolysin O (SLO). Enzymes secretions were indicated by detecting total protein secretions. Intracellular Ca2+([Ca2+](i)) was measured using Fluo-3 in SPEX spectrofluorimeter. RESULTS: The inhibition of GDP on SA( I) stimulated enzymes secretion increased with increasing GDP concentration. There were two peaks in the time course of increase in [Ca2+](i) evoked by SA(I) 10 mu mol/L. After adding GDP 5 mmol/L, [Ca2+](i) rose gradually without the two peaks. In permeabilized acini, the accumulation of enzymes stimulated by SA(I) in 30 min reduced by 57 % compared with intact acini. GDP 5 mmol/L decreased the initial rate of secretion. CONCLUSION: Inhibition of GDP on increase in [Ca2+ ii reduces SA(I) stimulated enzymes secretion in pancreatic acini.

语种英语
WOS记录号WOS:000169950000016
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60728
专题北京大学药学院
作者单位1.Nankai Univ, Dept Biophys, Tianjin 300071, Peoples R China
2.Beijing Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
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GB/T 7714
Yang, WX,Yu, Y,Zhang, WZ,et al. Inhibitory role of GDP on saikosaponin (I) stimulated enzymes secretion and rising of [Ca2+](i) in rat pancreatic acini[J]. ACTA PHARMACOLOGICA SINICA,2001,22(7):669-672.
APA Yang, WX.,Yu, Y.,Zhang, WZ.,Wang, H.,Li, XD.,...&Liang, H.(2001).Inhibitory role of GDP on saikosaponin (I) stimulated enzymes secretion and rising of [Ca2+](i) in rat pancreatic acini.ACTA PHARMACOLOGICA SINICA,22(7),669-672.
MLA Yang, WX,et al."Inhibitory role of GDP on saikosaponin (I) stimulated enzymes secretion and rising of [Ca2+](i) in rat pancreatic acini".ACTA PHARMACOLOGICA SINICA 22.7(2001):669-672.
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