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学科主题: 基础医学
题名:
Transduction of dendritic cells with recombinant adenovirus encoding HCA661 activates autologous cytotoxic T lymphocytes to target hepatoma cells
作者: Chan, RCF; Pang, XW; Wang, YD; Chen, WF; Xie, Y
关键词: hepatocellular carcinoma ; hepatoma ; liver cancer ; HCA661 ; dendritic cell ; recombinant adenovirus ; cytotoxic T lymphocytes ; tumour vaccine
刊名: BRITISH JOURNAL OF CANCER
发表日期: 2004-04-19
DOI: 10.1038/sj.bjc.6601706
卷: 90, 期:8, 页:1636-1643
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: CELLULAR IMMUNE-RESPONSES ; HUMAN HEPATOCELLULAR-CARCINOMA ; EPIDERMAL LANGERHANS CELLS ; CANCER-IMMUNOTHERAPY ; GENE-THERAPY ; PHASE-I ; ANTIGENS ; IDENTIFICATION ; VACCINATION ; MELANOMA
英文摘要:

Transduction of recombinant adenovirus into dendritic cells (DCs) is a promising new tool for cancer vaccine development. Here, we report that an adenovirus vector carrying hepatocellular carcinoma (HCC) antigen HCA661 and infected into DCs generates T-cell immunity against hepatoma cells. HCA661 is a novel cancer/testis (CT) antigen screened by SEREX from sera of an HCC patient. We constructed a recombinant adenovirus expressing the full-length cDNA of HCA661 gene and then transduced immature DCs, which had been generated with GM-CSF and IL-4 from peripheral blood mononuclear cell of HLA-A2(+) healthy donors. The resulting adenovirus-transduced DCs differentiated in the presence of monocyte-conditioned medium and poly [I] : poly [C], expressing the surface markers of mature DCs, including CD83, CD80, CD86 and HLA-DR. After maturation, the transduced DCs transcribed HCA661 mRNA and were able to prime the naive T cells to become cytotoxic T lymphocytes (CTLs). Intracellular flow cytometry and enzyme-linked immunospot assay showed that these CTLs were able to target a hepatoma cell line, HepG2, which is HLA-A2 and HCA661 positive. In summary, we found that this recombinant adenovirus can help to induce DC maturation and these mature DCs can activate T cells to target hepatoma cells. Therefore, this recombinant adenovirus may have potential for use in liver cancer immunotherapy.

语种: 英语
WOS记录号: WOS:000221129200028
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60736
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Hong Kong Univ Sci & Technol, Dept Biol, Kowloon, Hong Kong, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100083, Peoples R China

Recommended Citation:
Chan, RCF,Pang, XW,Wang, YD,et al. Transduction of dendritic cells with recombinant adenovirus encoding HCA661 activates autologous cytotoxic T lymphocytes to target hepatoma cells[J]. BRITISH JOURNAL OF CANCER,2004,90(8):1636-1643.
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