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学科主题基础医学
Transduction of dendritic cells with recombinant adenovirus encoding HCA661 activates autologous cytotoxic T lymphocytes to target hepatoma cells
Chan, RCF; Pang, XW; Wang, YD; Chen, WF; Xie, Y
关键词hepatocellular carcinoma hepatoma liver cancer HCA661 dendritic cell recombinant adenovirus cytotoxic T lymphocytes tumour vaccine
刊名BRITISH JOURNAL OF CANCER
2004-04-19
DOI10.1038/sj.bjc.6601706
90期:8页:1636-1643
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]CELLULAR IMMUNE-RESPONSES ; HUMAN HEPATOCELLULAR-CARCINOMA ; EPIDERMAL LANGERHANS CELLS ; CANCER-IMMUNOTHERAPY ; GENE-THERAPY ; PHASE-I ; ANTIGENS ; IDENTIFICATION ; VACCINATION ; MELANOMA
英文摘要

Transduction of recombinant adenovirus into dendritic cells (DCs) is a promising new tool for cancer vaccine development. Here, we report that an adenovirus vector carrying hepatocellular carcinoma (HCC) antigen HCA661 and infected into DCs generates T-cell immunity against hepatoma cells. HCA661 is a novel cancer/testis (CT) antigen screened by SEREX from sera of an HCC patient. We constructed a recombinant adenovirus expressing the full-length cDNA of HCA661 gene and then transduced immature DCs, which had been generated with GM-CSF and IL-4 from peripheral blood mononuclear cell of HLA-A2(+) healthy donors. The resulting adenovirus-transduced DCs differentiated in the presence of monocyte-conditioned medium and poly [I] : poly [C], expressing the surface markers of mature DCs, including CD83, CD80, CD86 and HLA-DR. After maturation, the transduced DCs transcribed HCA661 mRNA and were able to prime the naive T cells to become cytotoxic T lymphocytes (CTLs). Intracellular flow cytometry and enzyme-linked immunospot assay showed that these CTLs were able to target a hepatoma cell line, HepG2, which is HLA-A2 and HCA661 positive. In summary, we found that this recombinant adenovirus can help to induce DC maturation and these mature DCs can activate T cells to target hepatoma cells. Therefore, this recombinant adenovirus may have potential for use in liver cancer immunotherapy.

语种英语
WOS记录号WOS:000221129200028
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60736
专题基础医学院_免疫学系
作者单位1.Hong Kong Univ Sci & Technol, Dept Biol, Kowloon, Hong Kong, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100083, Peoples R China
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Chan, RCF,Pang, XW,Wang, YD,et al. Transduction of dendritic cells with recombinant adenovirus encoding HCA661 activates autologous cytotoxic T lymphocytes to target hepatoma cells[J]. BRITISH JOURNAL OF CANCER,2004,90(8):1636-1643.
APA Chan, RCF,Pang, XW,Wang, YD,Chen, WF,&Xie, Y.(2004).Transduction of dendritic cells with recombinant adenovirus encoding HCA661 activates autologous cytotoxic T lymphocytes to target hepatoma cells.BRITISH JOURNAL OF CANCER,90(8),1636-1643.
MLA Chan, RCF,et al."Transduction of dendritic cells with recombinant adenovirus encoding HCA661 activates autologous cytotoxic T lymphocytes to target hepatoma cells".BRITISH JOURNAL OF CANCER 90.8(2004):1636-1643.
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