IR@PKUHSC  > 北京大学第三临床医学院  > 生殖医学中心
学科主题临床医学
In Vitro Fertilization on a Single-Oocyte Positioning System Integrated with Motile Sperm Selection and Early Embryo Development
Ma, Rui1,2,5; Xie, Lan1,2,5; Han, Chao1,2,5; Su, Kai3; Qiu, Tian1,2,5; Wang, Lei2; Huang, Guoliang1,2,5; Xing, Wanli1,2,5; Qao, Jie4; Wang, Jundong3; Cheng, Jing1,2,5
刊名ANALYTICAL CHEMISTRY
2011-04-15
DOI10.1021/ac103063g
83期:8页:2964-2970
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]MAMMALIAN EMBRYOS ; REMOVAL ; CULTURE ; MICROFLUIDICS ; PREGNANCIES ; TECHNOLOGY ; INJECTION ; INVITRO ; DEVICE ; MOUSE
英文摘要

In vitro fertilization (IVF) technology has been broadly applied to solve human infertility in recent years. However, the physical tools for IVF remain unchanged over several decades before microfluidic technology was introduced in this field. Here, we report a novel microdevice that integrates each step of IVF, including oocyte positioning, sperm screening, fertilization, medium replacement, and embryo culture. Oocytes can be singly positioned in a 4 x 4 array of octacolumn units. The four symmetrical straight channels, crossing at the oocyte positioning region, allowed efficient motile sperm selection and facilitated rapid medium replacement. The fertilization process and early embryonic development of the individual zygote was traced with microscopic recording and analyzed by in situ fluorescent staining. The murine sperm motility was increased from 60.8 +/- 3.4% to 96.1 +/- 1.9% through the screening channels. The embryo growth rate and blastocyst formation were similar between the routine Petri dish group and the microdevice group. The healthy blastocysts developed in the microdevice could be conveniently retrieved through a routine pipetting operation and used for further embryo transfer.

语种英语
WOS记录号WOS:000289223700019
引用统计
被引频次:36[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60742
专题北京大学第三临床医学院_生殖医学中心
作者单位1.Tsinghua Univ, Sch Med, Dept Biomed Engn, Med Syst Biol Res Ctr, Beijing 100084, Peoples R China
2.Natl Engn Res Ctr Beijing Biochip Technol, Beijing 102206, Peoples R China
3.Shanxi Agr Univ, Shanxi Key Lab Ecol Anim Sci & Environm Med, Taigu 030801, Shanxi, Peoples R China
4.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Ctr Reprod Med, Beijing 100191, Peoples R China
5.Tsinghua Univ, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China
推荐引用方式
GB/T 7714
Ma, Rui,Xie, Lan,Han, Chao,et al. In Vitro Fertilization on a Single-Oocyte Positioning System Integrated with Motile Sperm Selection and Early Embryo Development[J]. ANALYTICAL CHEMISTRY,2011,83(8):2964-2970.
APA Ma, Rui.,Xie, Lan.,Han, Chao.,Su, Kai.,Qiu, Tian.,...&Cheng, Jing.(2011).In Vitro Fertilization on a Single-Oocyte Positioning System Integrated with Motile Sperm Selection and Early Embryo Development.ANALYTICAL CHEMISTRY,83(8),2964-2970.
MLA Ma, Rui,et al."In Vitro Fertilization on a Single-Oocyte Positioning System Integrated with Motile Sperm Selection and Early Embryo Development".ANALYTICAL CHEMISTRY 83.8(2011):2964-2970.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Ma, Rui]的文章
[Xie, Lan]的文章
[Han, Chao]的文章
百度学术
百度学术中相似的文章
[Ma, Rui]的文章
[Xie, Lan]的文章
[Han, Chao]的文章
必应学术
必应学术中相似的文章
[Ma, Rui]的文章
[Xie, Lan]的文章
[Han, Chao]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。