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学科主题: 临床医学
题名:
In Vitro Fertilization on a Single-Oocyte Positioning System Integrated with Motile Sperm Selection and Early Embryo Development
作者: Ma, Rui1,2,5; Xie, Lan1,2,5; Han, Chao1,2,5; Su, Kai3; Qiu, Tian1,2,5; Wang, Lei2; Huang, Guoliang1,2,5; Xing, Wanli1,2,5; Qao, Jie4; Wang, Jundong3; Cheng, Jing1,2,5
刊名: ANALYTICAL CHEMISTRY
发表日期: 2011-04-15
DOI: 10.1021/ac103063g
卷: 83, 期:8, 页:2964-2970
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
关键词[WOS]: MAMMALIAN EMBRYOS ; REMOVAL ; CULTURE ; MICROFLUIDICS ; PREGNANCIES ; TECHNOLOGY ; INJECTION ; INVITRO ; DEVICE ; MOUSE
英文摘要:

In vitro fertilization (IVF) technology has been broadly applied to solve human infertility in recent years. However, the physical tools for IVF remain unchanged over several decades before microfluidic technology was introduced in this field. Here, we report a novel microdevice that integrates each step of IVF, including oocyte positioning, sperm screening, fertilization, medium replacement, and embryo culture. Oocytes can be singly positioned in a 4 x 4 array of octacolumn units. The four symmetrical straight channels, crossing at the oocyte positioning region, allowed efficient motile sperm selection and facilitated rapid medium replacement. The fertilization process and early embryonic development of the individual zygote was traced with microscopic recording and analyzed by in situ fluorescent staining. The murine sperm motility was increased from 60.8 +/- 3.4% to 96.1 +/- 1.9% through the screening channels. The embryo growth rate and blastocyst formation were similar between the routine Petri dish group and the microdevice group. The healthy blastocysts developed in the microdevice could be conveniently retrieved through a routine pipetting operation and used for further embryo transfer.

语种: 英语
WOS记录号: WOS:000289223700019
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60742
Appears in Collections:北京大学第三临床医学院_生殖医学中心_期刊论文

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作者单位: 1.Tsinghua Univ, Sch Med, Dept Biomed Engn, Med Syst Biol Res Ctr, Beijing 100084, Peoples R China
2.Natl Engn Res Ctr Beijing Biochip Technol, Beijing 102206, Peoples R China
3.Shanxi Agr Univ, Shanxi Key Lab Ecol Anim Sci & Environm Med, Taigu 030801, Shanxi, Peoples R China
4.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Ctr Reprod Med, Beijing 100191, Peoples R China
5.Tsinghua Univ, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China

Recommended Citation:
Ma, Rui,Xie, Lan,Han, Chao,et al. In Vitro Fertilization on a Single-Oocyte Positioning System Integrated with Motile Sperm Selection and Early Embryo Development[J]. ANALYTICAL CHEMISTRY,2011,83(8):2964-2970.
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