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学科主题临床医学
Molecular epidemiology and evolution of A(H1N1)pdm09 and H3N2 virus during winter 2012-2013 in Beijing, China
Fang, Qiongxuan1; Gao, Yan1; Chen, Meifang1; Guo, Xiaolin1; Yang, Xia1; Yang, Xiaohua2; Wei, Lai1
关键词A(H1N1)pdm09 H3N2 Phylogeny Molecular-genetic analysis
刊名INFECTION GENETICS AND EVOLUTION
2014-08-01
DOI10.1016/j.meegid.2014.05.034
26页:228-240
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Infectious Diseases
研究领域[WOS]Infectious Diseases
关键词[WOS]INFLUENZA-A VIRUSES ; HEMAGGLUTININ ; NEURAMINIDASE ; H1N1 ; INFECTION ; CHILDREN ; BINDING ; DRIFT
英文摘要

In order to evaluate the epidemiology of influenza A and its surface antigens (haemagglutinin [HA] and neuraminidase [NA]) for molecular epidemiology and evolution analysis during winter 2012-2013 in Beijing, China, we worked within the framework of the Chinese National Influenza Center and collected nasal swabs of patients presenting with influenza-like illness. We found that both A(H1N1)pdm09 (46.8%) and H3N2 (53.2%) viruses were the predominant strains during the 2012-2013 influenza epidemic. The peak phase started at the second week of 2013 and lasted about 1 month. We obtained HA and NA sequences of viruses from 44 patients by using Sanger sequencing. None of the strains had the oseltamivir resistance site H274Y. Phylogenetic analysis of 29 A(H1N1) pdm09 viruses showed a genetic drift from the vaccine strain A/California/07/2009 with mutations (H155Q/R and L178I) at the antigenic sites Ca and Sa of HA; the strains were classified into genetic groups 6 and 7 because of the presence of D114N, S160G, S202T, and A214T mutations in HA. H3N2 viruses formed seasonal phylogenetic clusters representative for each season from 2000 to 2013; 15 of the 2012-2013 H3N2 strains were assigned to the A/Victoria/361/2011 genetic clade with mutations at the antigenic sites A, B and C of HA, including R158K/G, N161S, Q172H, and N294K; the 2012-2013 strains with V239I, S61N, T64I, and A214S HA mutations were classified into subgroup 3C. The mutation of potential N-linked glycosylation residues at the antigenic sites of HA and around the enzymatic active center of NA may have increased viral pathogenicity by masking antigenic sites from immune recognition. Our data suggest that influenza vaccines are generally effective, but still provide suboptimal protection due to antigenic variation. This study increases the understanding of influenza A viruses in humans and is informative for future vaccine strain selection. (C) 2014 Elsevier B. V. All rights reserved.

语种英语
WOS记录号WOS:000339954500030
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60745
专题北京大学第二临床医学院_感染科
北京大学第二临床医学院_北京大学肝病研究所
作者单位1.Peking Univ, Peoples Hosp, Inst Hepatol, Dept Infect Dis, Beijing 100044, Peoples R China
2.Zhongshan Univ, Affiliated Hosp 3, Dept Infect Dis, Guangzhou 510630, Guangdong, Peoples R China
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Fang, Qiongxuan,Gao, Yan,Chen, Meifang,et al. Molecular epidemiology and evolution of A(H1N1)pdm09 and H3N2 virus during winter 2012-2013 in Beijing, China[J]. INFECTION GENETICS AND EVOLUTION,2014,26:228-240.
APA Fang, Qiongxuan.,Gao, Yan.,Chen, Meifang.,Guo, Xiaolin.,Yang, Xia.,...&Wei, Lai.(2014).Molecular epidemiology and evolution of A(H1N1)pdm09 and H3N2 virus during winter 2012-2013 in Beijing, China.INFECTION GENETICS AND EVOLUTION,26,228-240.
MLA Fang, Qiongxuan,et al."Molecular epidemiology and evolution of A(H1N1)pdm09 and H3N2 virus during winter 2012-2013 in Beijing, China".INFECTION GENETICS AND EVOLUTION 26(2014):228-240.
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