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Nuf2 is required for chromosome segregation during mouse oocyte meiotic maturation
Zhang, Teng1,2; Zhou, Yang2; Qi, Shu-Tao2; Wang, Zhen-Bo2; Qian, Wei-Ping3; Ouyang, Ying-Chun2; Shen, Wei1; Schatten, Heide4; Sun, Qing-Yuan1,2
关键词kinetochore-microtubule attachments meiosis Nuf2 oocyte spindle assembly checkpoint
刊名CELL CYCLE
2015-08-18
DOI10.1080/15384101.2015.1058677
14期:16页:2701-2710
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
英文摘要

Nuf2 plays an important role in kinetochore-microtubule attachment and thus is involved in regulation of the spindle assembly checkpoint in mitosis. In this study, we examined the localization and function of Nuf2 during mouse oocyte meiotic maturation. Myc(6)-Nuf2 mRNA injection and immunofluorescent staining showed that Nuf2 localized to kinetochores from germinal vesicle breakdown to metaphase I stages, while it disappeared from the kinetochores at the anaphase I stage, but relocated to kinetochores at the MII stage. Overexpression of Nuf2 caused defective spindles, misaligned chromosomes, and activated spindle assembly checkpoint, and thus inhibited chromosome segregation and metaphase-anaphase transition in oocyte meiosis. Conversely, precocious polar body extrusion was observed in the presence of misaligned chromosomes and abnormal spindle formation in Nuf2 knock-down oocytes, causing aneuploidy. Our data suggest that Nuf2 is a critical regulator of meiotic cell cycle progression in mammalian oocytes.

语种英语
WOS记录号WOS:000360295400028
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60747
专题北京大学深圳医院
作者单位1.Qingdao Agr Univ, Inst Reprod Sci, Coll Anim Sci & Technol, Qingdao, Peoples R China
2.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China
3.Peking Univ, Shenzhen Hosp, Dept Reprod Med, Med Ctr, Shenzhen, Guangdong, Peoples R China
4.Univ Missouri, Dept Vet Pathobiol, Columbia, MO USA
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GB/T 7714
Zhang, Teng,Zhou, Yang,Qi, Shu-Tao,et al. Nuf2 is required for chromosome segregation during mouse oocyte meiotic maturation[J]. CELL CYCLE,2015,14(16):2701-2710.
APA Zhang, Teng.,Zhou, Yang.,Qi, Shu-Tao.,Wang, Zhen-Bo.,Qian, Wei-Ping.,...&Sun, Qing-Yuan.(2015).Nuf2 is required for chromosome segregation during mouse oocyte meiotic maturation.CELL CYCLE,14(16),2701-2710.
MLA Zhang, Teng,et al."Nuf2 is required for chromosome segregation during mouse oocyte meiotic maturation".CELL CYCLE 14.16(2015):2701-2710.
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