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学科主题: 临床医学
题名:
Interaction of thymic stromal lymphopoietin, IL-33, and their receptors in epithelial cells in eosinophilic chronic rhinosinusitis with nasal polyps
作者: Liao, B.1; Cao, P. -P.1; Zeng, M.1; Zhen, Z.1,2; Wang, H.1; Zhang, Y. -N.1,3; Hu, C. -Y.1,4; Ma, J.1; Li, Z. -Y.1; Song, J.1; Liu, J. -X.1; Peng, L. -Y.1; Liu, Y.1; Ning, Q.5; Liu, Z.1
关键词: cross-regulation ; interleukin 33 ; receptor ; thymic stromal lymphopoietin ; type 2 T-helper cell response
刊名: ALLERGY
发表日期: 2015-09-01
DOI: 10.1111/all.12667
卷: 70, 期:9, 页:1169-1180
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Allergy ; Immunology
研究领域[WOS]: Allergy ; Immunology
关键词[WOS]: INNATE LYMPHOID-CELLS ; HOUSE-DUST MITE ; INCREASED EXPRESSION ; MAST-CELLS ; ST2 ; INFLAMMATION ; CYTOKINES ; RESPONSES ; MEDIATOR ; SYSTEM
英文摘要:

BackgroundThymic stromal lymphopoietin (TSLP), IL-25, and IL-33 system contribute to the initiation and development of Th2 responses. This study aimed to explore the involvement of TSLP, IL-25, IL-33, and their receptors in type 2 T-helper (Th) responses in chronic rhinosinusitis with nasal polyps (CRSwNPs) and their cross-regulation in human nasal epithelial cells (HNECs).

MethodsImmunohistochemistry, quantitative RT-PCR, ELISA, Bio-Plex assay, and flow cytometry were used to detect the expression of TSLP/common -like TSLP receptor (TSLPR)/IL-7 receptor (IL-7R), IL-25/IL-17B receptor (IL-17RB), and IL-33/membrane-bound ST2 (ST2L)/soluble ST2 (sST2) in sinonasal mucosa and HNECs. HNECs cultured at an air-liquid interface were used to explore the expression in regulation of these cytokine systems.

ResultsCompared with controls and noneosinophilic CRSwNP, the expression of TSLP/TSLPR/IL-7R and ST2L/sST2 was significantly increased in eosinophilic CRSwNP, predominantly in epithelial cells. In contrast, the expression of IL-33 and IL-25/IL-17RB was enhanced in epithelial cells in both eosinophilic and noneosinophilic CRSwNP compared to controls. The expression of TSLP, TSLPR, and ST2L was positively correlated with symptom and computer tomography scan scores in eosinophilic CRSwNP and with Th2 cytokine expression in sinonasal mucosa. The expression of ST2L was correlated with TSLP and its receptor expression. TSLP could induce ST2L expression that promoted IL-33-induced TSLP expression in HNECs. In addition, TSLP/TSLPR/IL-7R and ST2L could be induced by Th2 cytokines, while IL-25/IL-17RB and IL-33 could be upregulated by Th1/Th17 cytokines, in HNECs.

ConclusionsThe positive feedback loop between TSLP, IL-33 and their receptors, and Th2 cytokines may facilitate Th2-skewed inflammation in eosinophilic CRSwNP.

语种: 英语
所属项目编号: 81020108018 ; 81325006 ; 81200733 ; 81300812 ; 201202005 ; IRT_14R20 ; 2014BAI07B04
项目资助者: National Natural Science Foundation of China (NSFC) ; Ministry of Health of China ; Program for Changjiang Scholars and Innovative Research Team in University ; 12th five year science and technology support program
WOS记录号: WOS:000360447200015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60762
Appears in Collections:北京大学第一临床医学院_期刊论文

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作者单位: 1.Xian Childrens Hosp, Dept Ear Nose & Throat, Xian, Peoples R China
2.Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Dept Otolaryngol Head & Neck Surg, Wuhan 430030, Peoples R China
3.Peking Univ, Hosp 1, Dept Otolaryngol Head & Neck Surg, Beijing 100871, Peoples R China
4.Tianjin First Ctr Hosp, Dept Otolaryngol Head & Neck Surg, Tianjin, Peoples R China
5.Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Dept Infect Dis,Inst Infect Dis, Wuhan 430030, Peoples R China

Recommended Citation:
Liao, B.,Cao, P. -P.,Zeng, M.,et al. Interaction of thymic stromal lymphopoietin, IL-33, and their receptors in epithelial cells in eosinophilic chronic rhinosinusitis with nasal polyps[J]. ALLERGY,2015,70(9):1169-1180.
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