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学科主题: 临床医学
题名:
Effect of recombinant human granulocyte colony-stimulating factor on T-lymphocyte function and the mechanism of this effect
作者: Chen, SH; Li, X; Huang, XJ
关键词: recombinant human granulocyte colony-stimulating factor ; antigen-presenting cells ; costimulating factor ; T-lymphocyte ; graft-versus-host disease, acute
刊名: INTERNATIONAL JOURNAL OF HEMATOLOGY
发表日期: 2004-02-01
DOI: 10.1532/IJH97.A10227
卷: 79, 期:2, 页:178-184
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Hematology
研究领域[WOS]: Hematology
关键词[WOS]: BONE-MARROW ; STEM-CELLS ; BLOOD ; TRANSPLANTATION ; DONORS ; PROLIFERATION ; SUPPRESSION
英文摘要:

Whether recombinant human granulocyte colony-stimulating factor (rhG-CSF) affects lymphocyte function directly or indirectly is controversial. In this study, we found that T-cell proliferation was decreased considerably in response to phytohemagglutinin in donors who received rhG-CSF but was partly restored after monocytes were removed. Intracellular cytokine staining revealed that the interferon gamma-interleukin 4 ratio decreased by 5.97-fold in donor CD4(+) cells after rhG-CSF treatment. No effect of rhG-CSF on ex vivo T-cell function was observed. rhG-CSF indirectly induced significant quantitative and qualitative changes on lymphocytes, including a decrease in T-cell proliferation and type 2 helper T-cell polarization of the cytokine profile. Although monocytes suppressed T-cell proliferation, the suppressive activity induced by the quantitative change in monocyte numbers cannot completely account for the hyporesponsiveness of T-lymphocytes. We believe that there must be another mediating factor. In addition, the numbers and mean fluorescence intensities of CD14(+)CD86(+) cells and CD19(+)CD80(+) cells declined significantly in the peripheral blood after rhG-CSF treatment. Suboptimal amounts of stimulatory signals provided by low expression levels of B7 molecules on antigen-presenting cells (monocytes, B-lymphocytes) may help explain the alteration in T-cell proliferation. In addition, the absolute counts of CD3(+)CD4(-)CD8(-) cells in the peripheral blood were markedly increased and enriched in leukapheresis products following G-CSF treatment. These suppressor cells may contribute to T-cell hyporesponsiveness.

语种: 英语
WOS记录号: WOS:000189189200014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60788
Appears in Collections:北京大学第二临床医学院_血液科_期刊论文

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作者单位: Peking Univ, Inst Hematol, Peoples Hosp, Beijing 100044, Peoples R China

Recommended Citation:
Chen, SH,Li, X,Huang, XJ. Effect of recombinant human granulocyte colony-stimulating factor on T-lymphocyte function and the mechanism of this effect[J]. INTERNATIONAL JOURNAL OF HEMATOLOGY,2004,79(2):178-184.
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