北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学药学院  > 期刊论文
学科主题: 药学
题名:
The effect of complexation of Cu(II) with P6A peptide and its analogs on their thrombolytic activities
作者: Yang, Mei2; Cui, Guohui1; Zhao, Ming1; Wang, Chao2; Wang, Lili1,3; Liu, Hu1,3,4; Peng, Shiqi1,2
关键词: Cu(II)-peptide complex ; Self-assembly ; Thrombolytic activity
刊名: INTERNATIONAL JOURNAL OF PHARMACEUTICS
发表日期: 2008-10-01
DOI: 10.1016/j.ijpharm.2008.06.014
卷: 362, 期:1-2, 页:81-87
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: DEGRADATION PRODUCT PEPTIDE-6A ; COPPER(II) COMPLEXES ; BLOOD-FLOW ; CU(II)-DIPEPTIDE COMPLEXES ; PLASMINOGEN-ACTIVATOR ; CRYSTAL-STRUCTURES ; BINDING ; PENTAPEPTIDE ; PERSPECTIVES ; METABOLITES
英文摘要:

The complexation of Cu(II) with previously synthesized thrombolytic peptides, Pro-Ala-Lys (6), Arg-Pro-Ala-Lys (7), Ala-Arg-Pro-Ala-Lys (8), Gly-Arg-Pro-Ala-Lys (9) and Gln-Arg-Pro-Ala-Lys (10), resulted in the formation of complexes, Cu(II)-(Pro-Ala-Lys) (6-Cu), Cu(II)-(Arg-Pro-Ala-Lys) (7-Cu), Cu(II)-(Ala-Arg-Pro-Ala-Lys) (8-Cu), Cu(II)-(Gly-Arg-Pro-Ala-Lys) (9-Cu) and Cu(II)-(Gln-Arg-Pro-Ala-Lys) (10-Cu), which was confirmed by UV-Vis, circular dichroism and ESI-MS analyses. Complexes (6-10)-Cu in normal saline (NS) were found to be able to assemble into aggregates, and the size of the aggregates were measured for the next eight consecutive days. It was found that on the 8th day, the diameters of (6-10)-Cu aggregates ranged from 179.21 +/- 38.33 nm to 293.46 +/- 51.07 mn. The Zeta potentials of (6-10)-Cu aggregates in NS were also examined and it was found that aggregates of (6, 8, 10)-Cu were negatively charged, and 7-Cu and 9-Cu were positively charged. The powders of (6-10)-Cu were analyzed by transmission electron microscopy and were found to have mean particle sizes of 8-15 nm. The in vitro euglobulin lysis, vasodilation and thrombolytic assays indicated that complexation With Cu(II) resulted in a significant increase in the activities of 6-10. The in vivo thrombolytic assays revealed that complexation with Cu(II) resulted in a significant enhancement in the in vivo thrombolytic activities for 6, 7,8 and 10 at 10 mu mol/kg, and 9 at 1 mu mol/kg and 0.1 mu mol/kg, respectively. These findings suggested that the self-assembly of the Cu(II)-peptide complexes into nano-scale aggregates was beneficial in improving the thrombolytic activity of the peptides. (C) 2008 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 2006CB708501 ; 20772082
项目资助者: Beijing Key Laboratory Program ; Small Molecular Drugs ; 973 Project of China ; Natural Scientific Foundation of China
WOS记录号: WOS:000263080700013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60796
Appears in Collections:北京大学药学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Capital Med Univ, Coll Pharmaceut Sci, Beijing 100069, Peoples R China
2.Peking Univ, Coll Pharmaceut Sci, Beijing 100083, Peoples R China
3.Mem Univ Newfoundland, Sch Pharm, St John, NF A1B 3V6, Canada
4.Mem Univ Newfoundland, Fac Sci, Dept Biochem, St John, NF A1B 3V6, Canada

Recommended Citation:
Yang, Mei,Cui, Guohui,Zhao, Ming,et al. The effect of complexation of Cu(II) with P6A peptide and its analogs on their thrombolytic activities[J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS,2008,362(1-2):81-87.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Yang, Mei]'s Articles
[Cui, Guohui]'s Articles
[Zhao, Ming]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Yang, Mei]‘s Articles
[Cui, Guohui]‘s Articles
[Zhao, Ming]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace