IR@PKUHSC  > 北京大学第一临床医学院  > 皮肤性病科
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Development and Evaluation of a Novel Real-Time PCR for Pan-Dermatophyte Detection in Nail Specimens
Gong, Jie1,2,3; Ran, Menglong1; Wang, Xiaowen1,2,3; Wan, Zhe1,2,3; Li, Ruoyu1,2,3
关键词Pan-dermatophyte Tinea unguium Fungal infection Dermatophyte PCR
刊名MYCOPATHOLOGIA
2016-02-01
DOI10.1007/s11046-015-9915-0
181期:1-2页:51-57
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Mycology
研究领域[WOS]Mycology
关键词[WOS]POLYMERASE-CHAIN-REACTION ; SUSPECTED ONYCHOMYCOSIS ; DIAGNOSIS ; IDENTIFICATION ; INFECTIONS ; RUBRUM ; ASSAY ; DNA
英文摘要

An accurate diagnosis of tinea unguium is necessary for the selection of antimycotics and successful treatment. To rapidly and accurately identify the aetiological agents causing tinea unguium, we improved upon the conventional boiling method for DNA extraction and developed a novel real-time PCR detection system that includes two assays. The two assays, based on the amplification of ribosomal internal transcribed spacer regions and 28S rDNA, were designed to detect pan-dermatophyte and Trichophyton rubrum, respectively. The analytical sensitivities of both assays permitted the detection of ten copies of plasmid DNA template. The analytical specificity of the detection system was confirmed using 11 dermatophyte strains and 25 non-dermatophyte strains. In total, 165 nail specimens were examined by microscopy, culture, conventional PCR, and the novel real-time PCR method. Real-time PCR gave positive results in 47.3 % of the specimens (78), a rate exceeding those obtained using microscopy (72, 43.6 %), conventional PCR (69, 41.8 %), and culture (49, 29.7 %). All conventional PCR-positive specimens were detected by real-time PCR, and real-time PCR detected nine specimens that were missed by conventional PCR. The results from latent class analysis, and further calculations, showed that real-time PCR was the most sensitive method, but the diagnostic specificity of the four approaches was equivalent. In particular, molecular approaches may be more effective than microscopy and culture when the clinical symptoms of tinea unguium are not evident.

语种英语
WOS记录号WOS:000366371500007
项目编号Z121107009212026 ; 2013ZX10004612-002
资助机构Beijing Key Laboratory of Molecular Diagnosis of Dermatoses ; National Science and Technology Key Projects on Major Infectious Diseases Such as HIV/AIDS, Viral Hepatitis Prevention and Treatment during the 12th 5-year plan period of the Ministry of Science and Technology of China
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60808
专题北京大学第一临床医学院_皮肤性病科
作者单位1.Beijing Key Lab Mol Diag Dermatoses, Beijing 100034, Peoples R China
2.Peking Univ, Res Ctr Med Mycol, Beijing 100034, Peoples R China
3.Peking Univ, Hosp 1, Dept Dermatol, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Gong, Jie,Ran, Menglong,Wang, Xiaowen,et al. Development and Evaluation of a Novel Real-Time PCR for Pan-Dermatophyte Detection in Nail Specimens[J]. MYCOPATHOLOGIA,2016,181(1-2):51-57.
APA Gong, Jie,Ran, Menglong,Wang, Xiaowen,Wan, Zhe,&Li, Ruoyu.(2016).Development and Evaluation of a Novel Real-Time PCR for Pan-Dermatophyte Detection in Nail Specimens.MYCOPATHOLOGIA,181(1-2),51-57.
MLA Gong, Jie,et al."Development and Evaluation of a Novel Real-Time PCR for Pan-Dermatophyte Detection in Nail Specimens".MYCOPATHOLOGIA 181.1-2(2016):51-57.
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