IR@PKUHSC  > 北京大学基础医学院
学科主题基础医学
PROTECTIVE EFFECTS OF THROMBOMODULIN ON MICROVASCULAR PERMEABILITY AFTER SUBARACHNOID HEMORRHAGE IN MOUSE MODEL
Xu, T.1; Zhang, W. -G.1; Sun, J.1; Zhang, Y.1; Lu, J. -F.1; Han, H. -B.2; Zhou, C. -M.1; Yan, J. -H.1,3
关键词microvascular permeability mouse subarachnoid hemorrhage thrombomodulin
刊名NEUROSCIENCE
2015-07-23
DOI10.1016/j.neuroscience.2015.04.058
299页:18-27
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences
资助者National Natural Science Foundation of China ; Seeding Grant for Medicine and Engineering Sciences of Peking University ; National Natural Science Foundation of China ; Seeding Grant for Medicine and Engineering Sciences of Peking University
研究领域[WOS]Neurosciences & Neurology
关键词[WOS]EARLY BRAIN-INJURY ; CEREBRAL-ARTERY OCCLUSION ; ACTIVATED PROTEIN-C ; RAT MODEL ; ISCHEMIC-STROKE ; BARRIER DISRUPTION ; BLOOD-COAGULATION ; THROMBIN ; EDEMA ; HEMOSTASIS
英文摘要

The enhanced vascular permeability is a major early brain injury following subarachnoid hemorrhage (SAH). However, its mechanism is not clear yet. In this work, we explored its potential mechanism and investigated the roles of thrombomodulin (TM) in maintaining microvascular integrity after SAH. SAH models were established in adult male ICR mice (28-32 g) by endovascular perforation. TM was immediately administered by femoral vein injection following SAH. The brain water content, Evans Blue content and neurological functions were evaluated. Brain edema was also detected by magnetic resonance imaging (MRI) (T2 map). The siRNA technique, enzyme-linked immunosorbent assay (ELISA), immunofluorescence staining and western blotting were performed to explore the potential mechanism of TM treatment. The number of microthrombi in the hippocampus microvessels was also recorded. TM significantly decreased brain water content and Evans Blue content, alleviated brain edema and neurological deficits after SAH. The plasma concentration of activated protein C was increased after TM treatment. In addition, the levels of phospho-p38MAPK, phospho-p53, cleaved caspase-3, phospho-NF-kappa B (p65) were markedly decreased. Additionally, the loss of VE-cadherin and Occludin (markers of vascular integrity) and the number of microthrombi in the hippocampus were also reduced. Our results indicated that TM has protective effects on preserving microvascular integrity following SAH partly through preserving endothelial junction proteins and quenching apoptosis/inflammation in endothelial cells via blocking p38MAPK-p53/NF-kappa B (p65) pathway. (C) 2015 IBRO. Published by Elsevier Ltd. All rights reserved.

语种英语
所属项目编号31471028 ; 31271280 ; 2014-ME-02
资助者National Natural Science Foundation of China ; Seeding Grant for Medicine and Engineering Sciences of Peking University ; National Natural Science Foundation of China ; Seeding Grant for Medicine and Engineering Sciences of Peking University
WOS记录号WOS:000355335500002
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60821
Collection北京大学基础医学院
作者单位1.Peking Univ, Sch Basic Med Sci, Dept Anat & Histol, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Dept Radiol, Beijing 100191, Peoples R China
3.Beijing Key Lab Magnet Resonance Imaging Technol, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Xu, T.,Zhang, W. -G.,Sun, J.,et al. PROTECTIVE EFFECTS OF THROMBOMODULIN ON MICROVASCULAR PERMEABILITY AFTER SUBARACHNOID HEMORRHAGE IN MOUSE MODEL[J]. NEUROSCIENCE,2015,299:18-27.
APA Xu, T..,Zhang, W. -G..,Sun, J..,Zhang, Y..,Lu, J. -F..,...&Yan, J. -H..(2015).PROTECTIVE EFFECTS OF THROMBOMODULIN ON MICROVASCULAR PERMEABILITY AFTER SUBARACHNOID HEMORRHAGE IN MOUSE MODEL.NEUROSCIENCE,299,18-27.
MLA Xu, T.,et al."PROTECTIVE EFFECTS OF THROMBOMODULIN ON MICROVASCULAR PERMEABILITY AFTER SUBARACHNOID HEMORRHAGE IN MOUSE MODEL".NEUROSCIENCE 299(2015):18-27.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
谷歌学术
谷歌学术Similar articles in
[Xu, T.]'s Articles
[Zhang, W. -G.]'s Articles
[Sun, J.]'s Articles
百度学术
百度学术Similar articles in
[Xu, T.]'s Articles
[Zhang, W. -G.]'s Articles
[Sun, J.]'s Articles
必应学术
必应学术Similar articles in
[Xu, T.]'s Articles
[Zhang, W. -G.]'s Articles
[Sun, J.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.