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学科主题: 基础医学
题名:
Mouse embryonic stem cell-derived cardiomyocytes express functional adrenoceptors
作者: Yang, Hongtao2; Zhang, Yongzhen2; Liu, Zhiqiang1; Chen, Ping1; Ma, Kangtao1; Zhou, Chunyan1
关键词: embryonic stem cells ; cardiomyocytes ; adrenoceptors ; muscarmic receptor ; tyrosine hydroxylase ; extracellular responsive kinase
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2008-04-18
DOI: 10.1016/j.bbrc.2008.02.014
卷: 368, 期:4, 页:887-892
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: BETA-ADRENERGIC-RECEPTOR ; HEART-RATE ; RAT-HEART ; DIFFERENTIATION ; CATECHOLAMINES ; PACEMAKING ; CYCLASE ; SUBTYPE
英文摘要:

The cardiogenic capacity of embryonic stem (ES) cells has been well-investigated. However, little is known about the development of adrenoceptor (AR) systems during the process of ES cell differentiation, which are critically important in cardiac physiology and pharmacology. In this present study, we investigated the expression profile of adrenoceptor subtypes, beta-adrenergic modulation of muscarinic receptors and adrenoceptor-related signaling in cardiomyocytes derived from ES cells (ESCMs).

Reverse transcription-polymerase chain reaction revealed that undifferentiated mouse ES cells expressed alpha(1A)-, alpha(1B)-, alpha(1D)- and beta(2)-AR mRNA. However, beta(1)-AR was only expressed after vitamin C induction. The expressions of alpha(1A)-, alpha(1D)- and beta(1)-ARs increased significantly while alpha(1B)- and beta(2)-ARs showed no significant change during the differentiation process. Furthermore, we detected the expression of tyrosine hydroxylase. Both alpha(1)-AR and beta-AR could activate extracellular, responsive kinase in ESCMs. Isoprenaline could inhibit the expression of M-2 muscarinic receptor protein. CGP20712A, a beta(1)-AR antagonist, up-regulated the expression of M-2 muscarinic receptor while ICI118551, a beta(2)-AR antagonist, showed no effect.

These results indicated that functional adrenoceptors and tyrosine hydroxylase, a critical enzyme in catecholamine biosynthesis, were differentially expressed in ESCMs. Adrenoceptor-related signaling pathways and beta-adrenergic modulation of muscarinic receptors were established during differentiation. (C) 2008 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000254106700009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60823
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Peking Univ, Dept Biochem & Mol Biol, Sch Basic Med Sci, Beijing 100083, Peoples R China
2.Peking Univ, Dept Cardiol, Hosp 3, Beijing 100083, Peoples R China

Recommended Citation:
Yang, Hongtao,Zhang, Yongzhen,Liu, Zhiqiang,et al. Mouse embryonic stem cell-derived cardiomyocytes express functional adrenoceptors[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2008,368(4):887-892.
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