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Mouse embryonic stem cell-derived cardiomyocytes express functional adrenoceptors
Yang, Hongtao2; Zhang, Yongzhen2; Liu, Zhiqiang1; Chen, Ping1; Ma, Kangtao1; Zhou, Chunyan1
关键词embryonic stem cells cardiomyocytes adrenoceptors muscarmic receptor tyrosine hydroxylase extracellular responsive kinase
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2008-04-18
DOI10.1016/j.bbrc.2008.02.014
368期:4页:887-892
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]BETA-ADRENERGIC-RECEPTOR ; HEART-RATE ; RAT-HEART ; DIFFERENTIATION ; CATECHOLAMINES ; PACEMAKING ; CYCLASE ; SUBTYPE
英文摘要

The cardiogenic capacity of embryonic stem (ES) cells has been well-investigated. However, little is known about the development of adrenoceptor (AR) systems during the process of ES cell differentiation, which are critically important in cardiac physiology and pharmacology. In this present study, we investigated the expression profile of adrenoceptor subtypes, beta-adrenergic modulation of muscarinic receptors and adrenoceptor-related signaling in cardiomyocytes derived from ES cells (ESCMs).

Reverse transcription-polymerase chain reaction revealed that undifferentiated mouse ES cells expressed alpha(1A)-, alpha(1B)-, alpha(1D)- and beta(2)-AR mRNA. However, beta(1)-AR was only expressed after vitamin C induction. The expressions of alpha(1A)-, alpha(1D)- and beta(1)-ARs increased significantly while alpha(1B)- and beta(2)-ARs showed no significant change during the differentiation process. Furthermore, we detected the expression of tyrosine hydroxylase. Both alpha(1)-AR and beta-AR could activate extracellular, responsive kinase in ESCMs. Isoprenaline could inhibit the expression of M-2 muscarinic receptor protein. CGP20712A, a beta(1)-AR antagonist, up-regulated the expression of M-2 muscarinic receptor while ICI118551, a beta(2)-AR antagonist, showed no effect.

These results indicated that functional adrenoceptors and tyrosine hydroxylase, a critical enzyme in catecholamine biosynthesis, were differentially expressed in ESCMs. Adrenoceptor-related signaling pathways and beta-adrenergic modulation of muscarinic receptors were established during differentiation. (C) 2008 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000254106700009
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被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60823
专题基础医学院
作者单位1.Peking Univ, Dept Biochem & Mol Biol, Sch Basic Med Sci, Beijing 100083, Peoples R China
2.Peking Univ, Dept Cardiol, Hosp 3, Beijing 100083, Peoples R China
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GB/T 7714
Yang, Hongtao,Zhang, Yongzhen,Liu, Zhiqiang,et al. Mouse embryonic stem cell-derived cardiomyocytes express functional adrenoceptors[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2008,368(4):887-892.
APA Yang, Hongtao,Zhang, Yongzhen,Liu, Zhiqiang,Chen, Ping,Ma, Kangtao,&Zhou, Chunyan.(2008).Mouse embryonic stem cell-derived cardiomyocytes express functional adrenoceptors.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,368(4),887-892.
MLA Yang, Hongtao,et al."Mouse embryonic stem cell-derived cardiomyocytes express functional adrenoceptors".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 368.4(2008):887-892.
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