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学科主题: 临床医学
题名:
Diagnostic significance of measuring antibodies to cyclic type 3 muscarinic acetylcholine receptor peptides in primary Sjogren′s syndrome
作者: He, Jing; Guo, Jian-ping; Ding, Yan; Li, Ying-ni; Pan, Si-si; Liu, Yanying; Li, Zhan-guo
关键词: Sjogren syndrome ; Type 3 muscarinic acetylcholine receptor ; Antibodies ; ESSDAI score
刊名: RHEUMATOLOGY
发表日期: 2011-05-01
DOI: 10.1093/rheumatology/keq420
卷: 50, 期:5, 页:879-884
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Rheumatology
研究领域[WOS]: Rheumatology
关键词[WOS]: EXTRACELLULAR LOOP ; REVISED CRITERIA ; M3 RECEPTOR ; AUTOANTIBODIES ; CLASSIFICATION ; MICE
英文摘要:

Methods. Sequences of the first to fourth cycle-M3R (c1M3R-c4M3R)-derived peptide was synthesized by a solid-phase technique on an Applied Biosytems Peptide Synthesizer. Synthesized cM3R peptide (cM3RP) was used as substrate in an ELISA to detect IgG anti-cM3RP antibodies in serum samples of patients and controls. The clinical and biological parameters of the diseases were also evaluated. The EULAR SS disease activity index (ESSDAI) score was used to measure disease activity in patients with primary SS.

Results. (i) Anti-c2M3RP antibodies were highly prevalent in pSS patients, and the titre is much higher than anti-c1,3,4M3RP antibodies. (ii) The prevalence of anti-c2M3RP antibodies in pSS, SLE, RA and healthy controls was 62.2, 7.1, 5.3 and 1.6%, respectively. The prevalence of anti-linear-2-M3RP antibodies in pSS, SLE and RA patients and healthy controls were 56.1, 20.0, 14.7 and 9.4%. (iii) The specificity of anti-c2M3RP antibodies was 95.1%, much higher than that of linear polypeptide (84.7%) for pSS diagnosis. (iv) In pSS patients, anti-c2M3RP positivity had significantly increased frequency in patients who were RF or ANA positive, and had several haematological abnormalities, such as leucopenia, anaemia and thrombocytopenia. Furthermore, the ESSDAI score was significantly higher in anti-c2M3RP-positive pSS patients (P < 0.05).

Conclusion. Anti-c2M3RP antibody was highly specific for patients with pSS. The presence of anti-c2M3RP antibody in pSS indicates that c2M3RP may act as an autoantigen that may play a role in the pathogenesis of pSS.

语种: 英语
所属项目编号: 7102155
项目资助者: Beijing Natural Science Foundation
WOS记录号: WOS:000289840300010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60825
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: Peking Univ, Peoples Hosp, Dept Rheumatol & Immunol, Beijing 100044, Peoples R China

Recommended Citation:
He, Jing,Guo, Jian-ping,Ding, Yan,et al. Diagnostic significance of measuring antibodies to cyclic type 3 muscarinic acetylcholine receptor peptides in primary Sjogren&prime;s syndrome[J]. RHEUMATOLOGY,2011,50(5):879-884.
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