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Diagnostic significance of measuring antibodies to cyclic type 3 muscarinic acetylcholine receptor peptides in primary Sjogren′s syndrome
He, Jing; Guo, Jian-ping; Ding, Yan; Li, Ying-ni; Pan, Si-si; Liu, Yanying; Li, Zhan-guo
关键词Sjogren syndrome Type 3 muscarinic acetylcholine receptor Antibodies ESSDAI score
刊名RHEUMATOLOGY
2011-05-01
DOI10.1093/rheumatology/keq420
50期:5页:879-884
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Rheumatology
研究领域[WOS]Rheumatology
关键词[WOS]EXTRACELLULAR LOOP ; REVISED CRITERIA ; M3 RECEPTOR ; AUTOANTIBODIES ; CLASSIFICATION ; MICE
英文摘要

Methods. Sequences of the first to fourth cycle-M3R (c1M3R-c4M3R)-derived peptide was synthesized by a solid-phase technique on an Applied Biosytems Peptide Synthesizer. Synthesized cM3R peptide (cM3RP) was used as substrate in an ELISA to detect IgG anti-cM3RP antibodies in serum samples of patients and controls. The clinical and biological parameters of the diseases were also evaluated. The EULAR SS disease activity index (ESSDAI) score was used to measure disease activity in patients with primary SS.

Results. (i) Anti-c2M3RP antibodies were highly prevalent in pSS patients, and the titre is much higher than anti-c1,3,4M3RP antibodies. (ii) The prevalence of anti-c2M3RP antibodies in pSS, SLE, RA and healthy controls was 62.2, 7.1, 5.3 and 1.6%, respectively. The prevalence of anti-linear-2-M3RP antibodies in pSS, SLE and RA patients and healthy controls were 56.1, 20.0, 14.7 and 9.4%. (iii) The specificity of anti-c2M3RP antibodies was 95.1%, much higher than that of linear polypeptide (84.7%) for pSS diagnosis. (iv) In pSS patients, anti-c2M3RP positivity had significantly increased frequency in patients who were RF or ANA positive, and had several haematological abnormalities, such as leucopenia, anaemia and thrombocytopenia. Furthermore, the ESSDAI score was significantly higher in anti-c2M3RP-positive pSS patients (P < 0.05).

Conclusion. Anti-c2M3RP antibody was highly specific for patients with pSS. The presence of anti-c2M3RP antibody in pSS indicates that c2M3RP may act as an autoantigen that may play a role in the pathogenesis of pSS.

语种英语
WOS记录号WOS:000289840300010
项目编号7102155
资助机构Beijing Natural Science Foundation
引用统计
被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60825
专题北京大学第二临床医学院
北京大学第二临床医学院_风湿免疫科
作者单位Peking Univ, Peoples Hosp, Dept Rheumatol & Immunol, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
He, Jing,Guo, Jian-ping,Ding, Yan,et al. Diagnostic significance of measuring antibodies to cyclic type 3 muscarinic acetylcholine receptor peptides in primary Sjogren&prime;s syndrome[J]. RHEUMATOLOGY,2011,50(5):879-884.
APA He, Jing.,Guo, Jian-ping.,Ding, Yan.,Li, Ying-ni.,Pan, Si-si.,...&Li, Zhan-guo.(2011).Diagnostic significance of measuring antibodies to cyclic type 3 muscarinic acetylcholine receptor peptides in primary Sjogren′s syndrome.RHEUMATOLOGY,50(5),879-884.
MLA He, Jing,et al."Diagnostic significance of measuring antibodies to cyclic type 3 muscarinic acetylcholine receptor peptides in primary Sjogren′s syndrome".RHEUMATOLOGY 50.5(2011):879-884.
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