IR@PKUHSC  > 基础医学院  > 神经生物学系
学科主题基础医学
Lysine-Specific Demethylase 1 in Breast Cancer Cells Contributes to the Production of Endogenous Formaldehyde in the Metastatic Bone Cancer Pain Model of Rats
Liu, Jia1,2; Liu, Feng-Yu1,2; Tong, Zhi-Qian1,2,3; Li, Zhi-Hua1,2,4; Chen, Wen1,2; Luo, Wen-Hong5; Li, Hui5; Luo, Hong-Jun5; Tang, Yan6; Tang, Jun-Min6; Cai, Jie1,2; Liao, Fei-Fei1,2; Wan, You1,2,7
刊名PLOS ONE
2013-03-14
DOI10.1371/journal.pone.0058957
8期:3
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者National Natural Science Foundation of China ; Specialized Research Fund for Doctoral Program of Higher Education ; Key Project of Chinese Ministry of Education ; Beijing Municipal Commission of Education "Special Grants for Outstanding Ph.D. Program Tutors" ; National Natural Science Foundation of China ; Specialized Research Fund for Doctoral Program of Higher Education ; Key Project of Chinese Ministry of Education ; Beijing Municipal Commission of Education "Special Grants for Outstanding Ph.D. Program Tutors"
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]SPINAL NERVE LIGATION ; ALDEHYDE DEHYDROGENASE ALDH ; DYNAMIC-RANGE NEURONS ; ROOT GANGLION NEURONS ; NEUROPATHIC PAIN ; SERINE HYDROXYMETHYLTRANSFERASE ; DIMETHYLGLYCINE DEHYDROGENASE ; INFLAMMATORY NOCICEPTION ; DIFFERENT INTENSITIES ; ECTOPIC DISCHARGES
英文摘要

Background: Bone cancer pain seriously affects the quality of life of cancer patients. Our previous study found that endogenous formaldehyde was produced by cancer cells metastasized into bone marrows and played an important role in bone cancer pain. However, the mechanism of production of this endogenous formaldehyde by metastatic cancer cells was unknown in bone cancer pain rats. Lysine-specific demethylase 1 (LSD1) is one of the major enzymes catalyzing the production of formaldehyde. The expression of LSD1 and the concentration of formaldehyde were up-regulated in many high-risk tumors.

Objective: This study aimed to investigate whether LSD1 in metastasized MRMT-1 breast cancer cells in bone marrows participated in the production of endogenous formaldehyde in bone cancer pain rats.

Methodology/Principal Findings: Concentration of the endogenous formaldehyde was measured by high performance liquid chromatography (HPLC). Endogenous formaldehyde dramatically increased in cultured MRMT-1 breast cancer cells in vitro, in bone marrows and sera of bone cancer pain rats, in tumor tissues and sera of MRMT-1 subcutaneous vaccination model rats in vivo. Formaldehyde at a concentration as low as the above measured (3 mM) induced pain behaviors in normal rats. The expression of LSD1 which mainly located in nuclei of cancer cells significantly increased in bone marrows of bone cancer pain rats from 14 d to 21 d after inoculation. Furthermore, inhibition of LSD1 decreased the production of formaldehyde in MRMT-1 cells in vitro. Intraperitoneal injection of LSD1 inhibitor pargyline from 3 d to 14 d after inoculation of MRMT-1 cancer cells reduced bone cancer pain behaviors.

Conclusion: Our data in the present study, combing our previous report, suggested that in the endogenous formaldehyde-induced pain in bone cancer pain rats, LSD1 in metastasized cancer cells contributed to the production of the endogenous formaldehyde.

语种英语
所属项目编号81171042 ; 81070893 ; 20110001110058 ; 109003
资助者National Natural Science Foundation of China ; Specialized Research Fund for Doctoral Program of Higher Education ; Key Project of Chinese Ministry of Education ; Beijing Municipal Commission of Education "Special Grants for Outstanding Ph.D. Program Tutors" ; National Natural Science Foundation of China ; Specialized Research Fund for Doctoral Program of Higher Education ; Key Project of Chinese Ministry of Education ; Beijing Municipal Commission of Education "Special Grants for Outstanding Ph.D. Program Tutors"
WOS记录号WOS:000316407400084
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60833
专题基础医学院_神经生物学系
作者单位1.Peking Univ, Neurosci Res Inst, Beijing 100871, Peoples R China
2.Peking Univ, Dept Neurobiol, Beijing 100871, Peoples R China
3.Chinese Acad Sci, Inst Biophys, Beijing 100080, Peoples R China
4.Zhengzhou Univ, Basic Med Coll, Dept Anat, Zhengzhou, Peoples R China
5.Shantou Univ, Coll Med, Cent Lab, Shantou, Peoples R China
6.Minist Hlth, Minist Educ, Key Lab Neurosci, Beijing, Peoples R China
7.Peking Univ, Sch Basic Med Sci, Dept Anat & Histol, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Liu, Jia,Liu, Feng-Yu,Tong, Zhi-Qian,et al. Lysine-Specific Demethylase 1 in Breast Cancer Cells Contributes to the Production of Endogenous Formaldehyde in the Metastatic Bone Cancer Pain Model of Rats[J]. PLOS ONE,2013,8(3).
APA Liu, Jia.,Liu, Feng-Yu.,Tong, Zhi-Qian.,Li, Zhi-Hua.,Chen, Wen.,...&Wan, You.(2013).Lysine-Specific Demethylase 1 in Breast Cancer Cells Contributes to the Production of Endogenous Formaldehyde in the Metastatic Bone Cancer Pain Model of Rats.PLOS ONE,8(3).
MLA Liu, Jia,et al."Lysine-Specific Demethylase 1 in Breast Cancer Cells Contributes to the Production of Endogenous Formaldehyde in the Metastatic Bone Cancer Pain Model of Rats".PLOS ONE 8.3(2013).
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Lysine-Specific Deme(2008KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Liu, Jia]的文章
[Liu, Feng-Yu]的文章
[Tong, Zhi-Qian]的文章
百度学术
百度学术中相似的文章
[Liu, Jia]的文章
[Liu, Feng-Yu]的文章
[Tong, Zhi-Qian]的文章
必应学术
必应学术中相似的文章
[Liu, Jia]的文章
[Liu, Feng-Yu]的文章
[Tong, Zhi-Qian]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Lysine-Specific Demethylase 1 in Breast Cancer Cells Contributes to the Production of Endogenous Formaldehyde in the Metasta.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。