IR@PKUHSC  > 北京大学深圳医院  > 皮肤性病科
学科主题临床医学
Identification of BANK1 polymorphisms by unlabelled probe high resolution melting: association with systemic lupus erythematosus susceptibility and autoantibody production in Han Chinese
Guan, Ming2,3; Yu, Bo4,5; Wan, Jun5,6; Zhang, Xinju2; Wu, Zhiyuan2; Zhong, Qili4; Zhang, Wei6,7; Zou, Hejian1
关键词BANK1 SLE Single nucleotide polymorphism High resolution melting
刊名RHEUMATOLOGY
2011-03-01
DOI10.1093/rheumatology/keq353
50期:3页:473-480
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Rheumatology
研究领域[WOS]Rheumatology
关键词[WOS]GENOME-WIDE ASSOCIATION ; HONG-KONG CHINESE ; FUNCTIONAL VARIANTS ; SIGNAL-TRANSDUCTION ; POPULATION ; DISEASE ; ITGAM ; GENE ; BURDEN ; TNFSF4
英文摘要

Methods. Genotyping of three variants in BANK1 was carried out by unlabelled probe high resolution melting (HRM) assay in 264 SLE cases and 268 controls in a Chinese Han population living in Shanghai region. The genotype frequencies of the detected polymorphisms were analysed in relation to the production of autoantibodies (ANA, anti-dsDNA, anti-RNP, anti-SSA, anti-SSB and anti-Smith) in SLE patients.

Results. Samples with the target genotypes were accurately detected and easily distinguishable by unlabelled probe HRM assay. The frequencies of the rs10516487 C allele and the rs17266594 T allele were significantly increased compared with the controls (C allele: 88.6 vs 83.2%, P = 0.011; T allele: 88.3 vs 83.2%, P = 0.019). However, the frequencies of the rs3733197 G allele were not associated with SLE (G allele: 79.9 vs 79.1%, P = 0.741). The rs10516487 and rs17266594 polymorphisms were significantly associated with high-titre ANA (epsilon 1 : 320) and production of anti-SSA antibodies in SLE patients compared with the control subjects.

Conclusions. Genotyping using unlabelled probes is a rapid, accurate and cost-effective closed-tube method. This study implies that rs10516487 and rs17266594 polymorphisms might contribute to individual susceptibility to SLE and influence the ANA/SSA autoantibody response in SLE patients in Chinese population.

语种英语
WOS记录号WOS:000287745600007
项目编号30872590
资助机构Natural Science Foundation of China ; Training Program Foundation for the Talents in Shanghai
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60843
专题北京大学深圳医院_皮肤性病科
作者单位1.Fudan Univ, Shanghai Med Coll, Huashan Hosp, Dept Rheumatol, Shanghai 200040, Peoples R China
2.Fudan Univ, Shanghai Med Coll, Huashan Hosp, Cent Lab, Shanghai 200040, Peoples R China
3.Fudan Univ, Shanghai Med Coll, Huashan Hosp, Dept Lab Med, Shanghai 200040, Peoples R China
4.Peking Univ, Shenzhen Hosp, Dept Dermatol, Shenzhen, Peoples R China
5.Shenzhen PKU HKUST Med Ctr, Shenzhen Key Lab Translat Med Dermatol, Shenzhen, Peoples R China
6.Hong Kong Univ Sci & Technol, Div Life Sci, Kowloon, Hong Kong, Peoples R China
7.Ji Nan Univ, JNU HKUST Joint Lab, Jinan, Guangdong, Peoples R China
推荐引用方式
GB/T 7714
Guan, Ming,Yu, Bo,Wan, Jun,et al. Identification of BANK1 polymorphisms by unlabelled probe high resolution melting: association with systemic lupus erythematosus susceptibility and autoantibody production in Han Chinese[J]. RHEUMATOLOGY,2011,50(3):473-480.
APA Guan, Ming.,Yu, Bo.,Wan, Jun.,Zhang, Xinju.,Wu, Zhiyuan.,...&Zou, Hejian.(2011).Identification of BANK1 polymorphisms by unlabelled probe high resolution melting: association with systemic lupus erythematosus susceptibility and autoantibody production in Han Chinese.RHEUMATOLOGY,50(3),473-480.
MLA Guan, Ming,et al."Identification of BANK1 polymorphisms by unlabelled probe high resolution melting: association with systemic lupus erythematosus susceptibility and autoantibody production in Han Chinese".RHEUMATOLOGY 50.3(2011):473-480.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Guan, Ming]的文章
[Yu, Bo]的文章
[Wan, Jun]的文章
百度学术
百度学术中相似的文章
[Guan, Ming]的文章
[Yu, Bo]的文章
[Wan, Jun]的文章
必应学术
必应学术中相似的文章
[Guan, Ming]的文章
[Yu, Bo]的文章
[Wan, Jun]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。