IR@PKUHSC  > 北京大学药学院
学科主题药学
A recombinant peptide, hirudin, potentiates the inhibitory effects of stealthy liposomal vinblastine on the growth and metastasis of melanoma
Guo, Rong-Rong; Liu, Yang; Lu, Wan-Liang1; Zhao, Ji-Hui; Wang, Xue-Qing; Zhang, Hua; Wang, Jian-Cheng; Zhang, Xuan; Zhang, Qiang
关键词metastasis melanoma recombinant hirudin stealthy liposomal vinblastine thrombin
刊名BIOLOGICAL & PHARMACEUTICAL BULLETIN
2008-04-01
31期:4页:696-702
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]AMINOCAPROIC ACID EACA ; TISSUE FACTOR ; BLOOD-COAGULATION ; TUMOR-CELLS ; IN-VITRO ; THROMBIN GENERATION ; ENDOTHELIAL-CELLS ; HEPARIN ; CANCER ; RECEPTOR
英文摘要

The metastasis of tumor cells is one of the major obstacles to successful clinical therapy. A treatment strategy by incorporating a specific inhibitor of thrombin, recombinant hirudin with stealthy liposomal vinblastine, was used in this study for inhibiting the metastasis of tumor cells and enhancing the efficacy of anti-tumor agents. In vitro cytotoxicity, cell adhesion to extracellular matrix (ECM) proteins, and cell invasion and migration assays were performed on human A375 melanoma cell line. In vivo measurement of coagulation parameters, inhibition of tumor growth, and inhibition of metastasis were assessed in female BALB/c mice. In vitro, vinblastine or stealthy liposomal vinblastine alone was effective to inhibit the growth of A375 cells. On the contrary, hirudin had no influence on either cytotoxicity when treating with hirudin alone or hirudin plus vinblastine. In addition, in vitro results showed that hirudin had no impact on the adhesion of tumor cells to extracellular matrix proteins, and metastasis and invasion of tumor cells. In mice, hirudin significantly inhibited the activity of thrombin. Furthermore, administered at the initial implantation of murine B16 melanoma cells, hirudin evidently delayed the growth of tumor, and depressed the occurrence of experimental lung metastasis. A subsequent administration of stealthy liposomal vinblastine resulted in further inhibiting growth and metastasis of tumor, indicating that hirudin plus stealthy liposomal vinblastine exhibited a significant anti-metastasis effect and slightly potent effect against tumor growth as compared with stealthy liposomal vinblastine alone. In conclusion, administration of recombinant hirudin followed by giving stealthy liposomal vinblastine may be beneficial for inhibiting the growth and metastasis of melanoma in vivo. The likely mechanism could be associated with inhibition of thrombin after administration of hirudin.

语种英语
WOS记录号WOS:000255503700029
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60845
专题北京大学药学院
作者单位1.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Guo, Rong-Rong,Liu, Yang,Lu, Wan-Liang,et al. A recombinant peptide, hirudin, potentiates the inhibitory effects of stealthy liposomal vinblastine on the growth and metastasis of melanoma[J]. BIOLOGICAL & PHARMACEUTICAL BULLETIN,2008,31(4):696-702.
APA Guo, Rong-Rong.,Liu, Yang.,Lu, Wan-Liang.,Zhao, Ji-Hui.,Wang, Xue-Qing.,...&Zhang, Qiang.(2008).A recombinant peptide, hirudin, potentiates the inhibitory effects of stealthy liposomal vinblastine on the growth and metastasis of melanoma.BIOLOGICAL & PHARMACEUTICAL BULLETIN,31(4),696-702.
MLA Guo, Rong-Rong,et al."A recombinant peptide, hirudin, potentiates the inhibitory effects of stealthy liposomal vinblastine on the growth and metastasis of melanoma".BIOLOGICAL & PHARMACEUTICAL BULLETIN 31.4(2008):696-702.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Guo, Rong-Rong]的文章
[Liu, Yang]的文章
[Lu, Wan-Liang]的文章
百度学术
百度学术中相似的文章
[Guo, Rong-Rong]的文章
[Liu, Yang]的文章
[Lu, Wan-Liang]的文章
必应学术
必应学术中相似的文章
[Guo, Rong-Rong]的文章
[Liu, Yang]的文章
[Lu, Wan-Liang]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。