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学科主题: 临床医学
题名:
Detection of anti-envoplakin and anti-periplakin autoantibodies by ELISA in patients with paraneoplastic pemphigus
作者: Huang, Yongchu; Li, Jing; Zhu, Xuejun
关键词: Envoplakin ; Periplakin ; ELISA ; PNP
刊名: ARCHIVES OF DERMATOLOGICAL RESEARCH
发表日期: 2009-10-01
DOI: 10.1007/s00403-008-0901-y
卷: 301, 期:10, 页:703-709
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dermatology
研究领域[WOS]: Dermatology
关键词[WOS]: LINKED-IMMUNOSORBENT-ASSAY ; RECOMBINANT DESMOGLEINS ; VULGARIS ANTIGEN ; BACULOVIRUS
英文摘要:

Paraneoplastic pemphigus patients (PNP) develop a group of autoantibodies, among which those against envoplakin and periplakin are almost always found. Epitope mapping has indicated that the linker subdomains of the proteins harbor the major antigenic sites recognized by PNP sera. In order to detect specific autoantibodies for the diagnosis of PNP, we expressed recombinant proteins containing linker subdomains of human periplakin and envoplakin in a human kidney cell line, and used them as the antigens for ELISAs. We found that all of the sera from 16 PNP patients recognized these two recombinant proteins by ELISA, and sera from 20 pemphigus vulgaris (PV), 12 pemphigus foliaceus (PF), 20 bullous pemphigoid (BP), 2 Castleman′s tumor without PNP and 20 normal controls showed negative results. We also expressed the extracellular domain of desmoglein 3 (Dsg3) in the cell line, and used this recombinant Dsg3 as the ELISA antigen. Only 11 of our 16 PNP sera were positive, and most PV sera were positive. Our findings indicate that ELISAs using the recombinant proteins containing linker subdomains of envoplakin and periplakin expressed in a human cell line as the antigens are highly sensitive and specific for the diagnosis of PNP.

语种: 英语
所属项目编号: 30671890
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000269916500002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60858
Appears in Collections:北京大学第一临床医学院_皮肤性病科_期刊论文

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作者单位: Peking Univ, Dept Dermatol, Hosp 1, Beijing 100034, Peoples R China

Recommended Citation:
Huang, Yongchu,Li, Jing,Zhu, Xuejun. Detection of anti-envoplakin and anti-periplakin autoantibodies by ELISA in patients with paraneoplastic pemphigus[J]. ARCHIVES OF DERMATOLOGICAL RESEARCH,2009,301(10):703-709.
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