IR@PKUHSC  > 北京大学第一临床医学院  > 皮肤性病科
学科主题临床医学
Detection of anti-envoplakin and anti-periplakin autoantibodies by ELISA in patients with paraneoplastic pemphigus
Huang, Yongchu; Li, Jing; Zhu, Xuejun
关键词Envoplakin Periplakin ELISA PNP
刊名ARCHIVES OF DERMATOLOGICAL RESEARCH
2009-10-01
DOI10.1007/s00403-008-0901-y
301期:10页:703-709
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dermatology
研究领域[WOS]Dermatology
关键词[WOS]LINKED-IMMUNOSORBENT-ASSAY ; RECOMBINANT DESMOGLEINS ; VULGARIS ANTIGEN ; BACULOVIRUS
英文摘要

Paraneoplastic pemphigus patients (PNP) develop a group of autoantibodies, among which those against envoplakin and periplakin are almost always found. Epitope mapping has indicated that the linker subdomains of the proteins harbor the major antigenic sites recognized by PNP sera. In order to detect specific autoantibodies for the diagnosis of PNP, we expressed recombinant proteins containing linker subdomains of human periplakin and envoplakin in a human kidney cell line, and used them as the antigens for ELISAs. We found that all of the sera from 16 PNP patients recognized these two recombinant proteins by ELISA, and sera from 20 pemphigus vulgaris (PV), 12 pemphigus foliaceus (PF), 20 bullous pemphigoid (BP), 2 Castleman′s tumor without PNP and 20 normal controls showed negative results. We also expressed the extracellular domain of desmoglein 3 (Dsg3) in the cell line, and used this recombinant Dsg3 as the ELISA antigen. Only 11 of our 16 PNP sera were positive, and most PV sera were positive. Our findings indicate that ELISAs using the recombinant proteins containing linker subdomains of envoplakin and periplakin expressed in a human cell line as the antigens are highly sensitive and specific for the diagnosis of PNP.

语种英语
WOS记录号WOS:000269916500002
项目编号30671890
资助机构National Natural Science Foundation of China
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60858
专题北京大学第一临床医学院_皮肤性病科
作者单位Peking Univ, Dept Dermatol, Hosp 1, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Huang, Yongchu,Li, Jing,Zhu, Xuejun. Detection of anti-envoplakin and anti-periplakin autoantibodies by ELISA in patients with paraneoplastic pemphigus[J]. ARCHIVES OF DERMATOLOGICAL RESEARCH,2009,301(10):703-709.
APA Huang, Yongchu,Li, Jing,&Zhu, Xuejun.(2009).Detection of anti-envoplakin and anti-periplakin autoantibodies by ELISA in patients with paraneoplastic pemphigus.ARCHIVES OF DERMATOLOGICAL RESEARCH,301(10),703-709.
MLA Huang, Yongchu,et al."Detection of anti-envoplakin and anti-periplakin autoantibodies by ELISA in patients with paraneoplastic pemphigus".ARCHIVES OF DERMATOLOGICAL RESEARCH 301.10(2009):703-709.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Huang, Yongchu]的文章
[Li, Jing]的文章
[Zhu, Xuejun]的文章
百度学术
百度学术中相似的文章
[Huang, Yongchu]的文章
[Li, Jing]的文章
[Zhu, Xuejun]的文章
必应学术
必应学术中相似的文章
[Huang, Yongchu]的文章
[Li, Jing]的文章
[Zhu, Xuejun]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。