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Analysis of gene expression patterns of ovarian cancer cell lines with different metastatic potentials
Bai, F; Feng, J; Cheng, Y; Shi, J; Yang, R; Cui, H
关键词metastasis microarray ovarian cancer RT-PCR Western blotting
刊名INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER
2006
16期:1页:202-209
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Obstetrics & Gynecology
研究领域[WOS]Oncology ; Obstetrics & Gynecology
关键词[WOS]NUCLEOSIDE DIPHOSPHATE KINASE ; MICROARRAY ; NM23 ; IDENTIFICATION ; CARCINOMAS
英文摘要

The objective of this study was to investigate the key genetic changes and molecular mechanisms in the process of invasion and metastasis of human epithelial ovarian cancers. The in vitro invasion assay was used to further testify that the human epithelial ovarian cancer cell line SKOV3.ip1 is more invasive and metastatic compared with its parental line SKOV3. A total of 17,000 human genome complementary DNA microarrays were used to compare the gene expression patterns of the two cell lines. Reverse transcription-polymerase chain reactions and Western blotting were performed to validate the results of the microarray. Totally, 1557 twofold differentially expressed genes were screened out by 17,000 human genome complementary DNA microarrays between the two cell lines, including some important genes such as, nm23, c-erbB-2, and other unknown genes or expressed sequence tags with remarkable fold changes. The results of the microarray experiment were further confirmed by reverse transcription-polymerase chain reactions and Western blotting of the nm23-H2 gene. SKOV3.ip1 is more invasive and metastatic than its parental line. The invasion and metastasis mechanism of epithelial ovarian cancer is a very complex process in which many important genes like nm23, c-erbB-2, as well as other unknown genes or expressed sequence tags were involved.

语种英语
WOS记录号WOS:000234800100033
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60869
专题北京大学第二临床医学院_妇科肿瘤中心
作者单位Peking Univ, Peoples Hosp, Gynecol Oncol Ctr, Beijing 100044, Peoples R China
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GB/T 7714
Bai, F,Feng, J,Cheng, Y,et al. Analysis of gene expression patterns of ovarian cancer cell lines with different metastatic potentials[J]. INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER,2006,16(1):202-209.
APA Bai, F,Feng, J,Cheng, Y,Shi, J,Yang, R,&Cui, H.(2006).Analysis of gene expression patterns of ovarian cancer cell lines with different metastatic potentials.INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER,16(1),202-209.
MLA Bai, F,et al."Analysis of gene expression patterns of ovarian cancer cell lines with different metastatic potentials".INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER 16.1(2006):202-209.
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