IR@PKUHSC  > 北京大学公共卫生学院
学科主题公共卫生
Association between Polymorphisms of DNA repair gene XRCC1 and DNA damage in asbestos-exposed workers
Zhao, XH; Jia, G; Liu, YQ; Liu, SW; Yan, L; Jin, Y; Lj, N
关键词asbestos asbestosis DNA damage XRCC1 DNA repair polymorphisms comet assays
刊名BIOMEDICAL AND ENVIRONMENTAL SCIENCES
2006-06-01
19期:3页:232-238
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Environmental Sciences ; Public, Environmental & Occupational Health
研究领域[WOS]Environmental Sciences & Ecology ; Public, Environmental & Occupational Health
关键词[WOS]COMET ASSAY ; CELLS ; RISK ; LUNG ; SENSITIVITY ; POPULATION ; FREQUENCY
英文摘要

Objective To compare the asbestos-induced DNA damage and repair capacities of DNA damage between 104 asbestos-exposed workers and 101 control workers in Qingdao City of China and to investigate the possible association between polymorphisms in codon 399 of XRCC1 and susceptibility to asbestosis. Methods DNA damage levels in peripheral blood lymphocytes were determined by comet assay, and XRCC1 genetic polymorphisms of DNA samples from 51 asbestosis cases and 53 non-asbestosis workers with a similar asbestos exposure history were analyzed by PCR/RFLP. Results The basal comet scores (3.95 +/- 2.95) were significantly higher in asbestos-exposed workers than in control workers (0.10 +/- 0.28). After 1 h H2O2 stimulation, DNA damage of lymphocytes exhibited different increases. After a 4 h repair period, the comet scores were 50.98 +/- 19.53 in asbestos-exposed workers and 18.32 +/- 12.04 in controls. The residual DNA damage (RD) was significantly greater (P < 0.01) in asbestos-exposed workers (35.62%) than in controls (27.75%). XRCC1 genetic polymorphism in 104 asbestos-exposed workers was not associated with increased risk of asbestosis. But compared with polymorphisms in the DNA repair gene XRCC1 (polymorphisms in codon 399) and the DNA damage induced by asbestos, the comet scores in asbestosis cases with Gln/Gln, Ghr/Arg, and Arg/Arg were 40.26 +/- 18.94, 38.03 +/- 28.22, and 32.0 +/- 11.65, respectively, which were higher than those in non-asbestosis workers with the same genotypes (25.58 +/- 11.08, 37.08 +/- 14.74, and 29.38 +/- 10.15). There were significant differences in the comet scores between asbestosis cases and non-asbestosis workers with Gln/Gln by Student′s t-test (P < 0.05 or 0.01). The comet scores were higher in asbestosis workers with Gln/Gln than in those with Arg/Arg and in non-asbestosis workers exposed to asbestos, but without statistically significant difference. Conclusions Exposure to asbestos may be related to DNA damage or the capacity of cells to repair H2O2-induced DNA damage. DNA repair gene XRCC1 codon 399 may be responsible for the inter-individual susceptibility in DNA damage and repair capacities.

语种英语
WOS记录号WOS:000238694500013
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60883
专题北京大学公共卫生学院
作者单位1.Inst Haidian Hlth Inspect & Supervis, Beijing 100080, Peoples R China
2.Beijing Union Univ, Beijing Key Lab Bioact Subst & Funct Foods, Beijing 100083, Peoples R China
3.Peking Univ, Sch Publ Hlth, Dept Occupat & Environm Hlth Sci, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Zhao, XH,Jia, G,Liu, YQ,et al. Association between Polymorphisms of DNA repair gene XRCC1 and DNA damage in asbestos-exposed workers[J]. BIOMEDICAL AND ENVIRONMENTAL SCIENCES,2006,19(3):232-238.
APA Zhao, XH.,Jia, G.,Liu, YQ.,Liu, SW.,Yan, L.,...&Lj, N.(2006).Association between Polymorphisms of DNA repair gene XRCC1 and DNA damage in asbestos-exposed workers.BIOMEDICAL AND ENVIRONMENTAL SCIENCES,19(3),232-238.
MLA Zhao, XH,et al."Association between Polymorphisms of DNA repair gene XRCC1 and DNA damage in asbestos-exposed workers".BIOMEDICAL AND ENVIRONMENTAL SCIENCES 19.3(2006):232-238.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhao, XH]的文章
[Jia, G]的文章
[Liu, YQ]的文章
百度学术
百度学术中相似的文章
[Zhao, XH]的文章
[Jia, G]的文章
[Liu, YQ]的文章
必应学术
必应学术中相似的文章
[Zhao, XH]的文章
[Jia, G]的文章
[Liu, YQ]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。