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学科主题: 基础医学
题名:
Regulation of osteopontin expression in a rat model of urolithiasis
作者: Chang, LS; Feng, T; Li, JX; Dou, CQ; Wei, J; Guo, YL
关键词: urolithiasis ; animal model ; osteopontin ; regulation
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2001-08-01
卷: 114, 期:8, 页:829-832
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: TESTOSTERONE ; CELLS
英文摘要:

Objective To investigate the relationship between the expression and regulation of osteopontin (OPN) and urolithiasis.

Methods Normal and stone model rats were treated with 1, 25-dihydroxyvitamin D-3 (D-3), vitamin K, testosterone or estradiol for 7 days, and the expression of osteopontin and its mRNA were detected with immunohistochemistry and Northern blot, respectively. Crystals deposited in rat kidneys were observed with a polarization microscope. The concentrations of crystal components in rat urine were determined.

Results The results showed that vitamin K, testosterone and estradiol up-regulated the expression of OPN mRNA and its protein, thus decreasing the precipitation of calcium oxalate in rat kidneys. D3 increased the concentration of calcium in urine, and accelerated the sedimentation of calcium oxalate in rat kidneys.

Conclusions These findings indicate that OPN may be an important macromolecule in the normal endogenous inhibition of the formation of urolithiasis. Vitamin K, testosterone and estradiol inhibit the formation of stones via up-regulating the expression of OPN in kidneys, while D3 over dose may accelerate the process.

语种: 英语
WOS记录号: WOS:000170838400011
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60896
Appears in Collections:基础医学院_生理学与病理生理学系_期刊论文

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作者单位: 1.Beijing Univ, Dept Physiol, Beijing 100083, Peoples R China
2.Beijing Univ, Affiliated Hosp 1, Inst Urol, Beijing 100034, Peoples R China

Recommended Citation:
Chang, LS,Feng, T,Li, JX,et al. Regulation of osteopontin expression in a rat model of urolithiasis[J]. CHINESE MEDICAL JOURNAL,2001,114(8):829-832.
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