IR@PKUHSC  > 北京大学口腔医学院  > 口腔病理科
学科主题口腔医学
Disruption of Wnt/beta-catenin Signaling in Odontoblasts and Cementoblasts Arrests Tooth Root Development in Postnatal Mouse Teeth
Zhang, Ran1,2; Yang, Guan2; Wu, Ximei3; Xie, Jing2; Yang, Xiao2; Li, Tiejun1
关键词Tooth root development Odontoblast Wnt/beta-catenin signaling mouse model
刊名INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
2013
DOI10.7150/ijbs.5476
9期:3页:228-236
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biology
资助者State Key Program of National Natural Science of China ; National Basic Research Program of China ; National Natural Science Foundation of China ; State Key Program of National Natural Science of China ; National Basic Research Program of China ; National Natural Science Foundation of China
研究领域[WOS]Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
关键词[WOS]BETA-CATENIN ; EXPRESSION ; CELLS ; DIFFERENTIATION ; DENTIN ; CROWN ; MICE
英文摘要

Tooth development undergoes a series of complex reciprocal interactions between dental epithelium and the underlying mesenchymal cells. Compared with the study in tooth crown formation, little is known about the molecular mechanism underlying the development of tooth roots. In the present study, we conditionally knock out beta-catenin gene (Ctnnb1) within developing odontoblasts and cementoblasts during the development of tooth roots, and observed rootless molars as well as incomplete incisors. Histological analyses revealed intact structure of molar crown and labial side of incisor, however, as for the molar roots and the lingual portion of incisor, the formation of dentin and periodontal tissues were greatly hampered. In situ hybridization experiments using probes of odontoblastic marker genes collagen type I, alpha 1 (Col1a1), osteocalcin (OC) and dentin sialophosphoprotein (Dspp) manifested striking undifferentiation of root odontoblasts in which Ctnnb1 was eliminated. Bromodeoxyuridine (BrdU) labeling and proliferating cell nuclear antigen (PCNA) immunohistochemical experiments also showed retarded proliferation of pre-odontoblasts in mutant mice. However, cell apoptosis was not affected. Additionally, a disrupted formation of cementoblasts, suggested by the absence of transcripts of bone sialoprotein (Bsp) in follicle mesenchyme, was also evident in mutant mice. Our study provides strong in vivo evidence to confirm that Wnt/beta-catenin signaling is functionally significant to root odontogenesis and cementogenesis during the tooth root development.

语种英语
所属项目编号81030018 ; 2012CB966904 ; 30900863 ; 81241062
资助者State Key Program of National Natural Science of China ; National Basic Research Program of China ; National Natural Science Foundation of China ; State Key Program of National Natural Science of China ; National Basic Research Program of China ; National Natural Science Foundation of China
WOS记录号WOS:000316996800001
Citation statistics
Cited Times:28[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60902
Collection北京大学口腔医学院_口腔病理科
作者单位1.Peking Univ, Sch & Hosp Stomatol, Dept Oral Pathol, Beijing 100081, Peoples R China
2.Inst Biotechnol, Genet Lab Dev & Dis, State Key Lab Prote, Beijing 100071, Peoples R China
3.Zhejiang Univ, Coll Med, Dept Pharmacol, Hangzhou 310003, Zhejiang, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Ran,Yang, Guan,Wu, Ximei,et al. Disruption of Wnt/beta-catenin Signaling in Odontoblasts and Cementoblasts Arrests Tooth Root Development in Postnatal Mouse Teeth[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2013,9(3):228-236.
APA Zhang, Ran,Yang, Guan,Wu, Ximei,Xie, Jing,Yang, Xiao,&Li, Tiejun.(2013).Disruption of Wnt/beta-catenin Signaling in Odontoblasts and Cementoblasts Arrests Tooth Root Development in Postnatal Mouse Teeth.INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,9(3),228-236.
MLA Zhang, Ran,et al."Disruption of Wnt/beta-catenin Signaling in Odontoblasts and Cementoblasts Arrests Tooth Root Development in Postnatal Mouse Teeth".INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 9.3(2013):228-236.
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