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学科主题: 口腔医学
题名:
Disruption of Wnt/beta-catenin Signaling in Odontoblasts and Cementoblasts Arrests Tooth Root Development in Postnatal Mouse Teeth
作者: Zhang, Ran1,2; Yang, Guan2; Wu, Ximei3; Xie, Jing2; Yang, Xiao2; Li, Tiejun1
关键词: Tooth root development ; Odontoblast ; Wnt/beta-catenin signaling ; mouse model
刊名: INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
发表日期: 2013
DOI: 10.7150/ijbs.5476
卷: 9, 期:3, 页:228-236
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
关键词[WOS]: BETA-CATENIN ; EXPRESSION ; CELLS ; DIFFERENTIATION ; DENTIN ; CROWN ; MICE
英文摘要:

Tooth development undergoes a series of complex reciprocal interactions between dental epithelium and the underlying mesenchymal cells. Compared with the study in tooth crown formation, little is known about the molecular mechanism underlying the development of tooth roots. In the present study, we conditionally knock out beta-catenin gene (Ctnnb1) within developing odontoblasts and cementoblasts during the development of tooth roots, and observed rootless molars as well as incomplete incisors. Histological analyses revealed intact structure of molar crown and labial side of incisor, however, as for the molar roots and the lingual portion of incisor, the formation of dentin and periodontal tissues were greatly hampered. In situ hybridization experiments using probes of odontoblastic marker genes collagen type I, alpha 1 (Col1a1), osteocalcin (OC) and dentin sialophosphoprotein (Dspp) manifested striking undifferentiation of root odontoblasts in which Ctnnb1 was eliminated. Bromodeoxyuridine (BrdU) labeling and proliferating cell nuclear antigen (PCNA) immunohistochemical experiments also showed retarded proliferation of pre-odontoblasts in mutant mice. However, cell apoptosis was not affected. Additionally, a disrupted formation of cementoblasts, suggested by the absence of transcripts of bone sialoprotein (Bsp) in follicle mesenchyme, was also evident in mutant mice. Our study provides strong in vivo evidence to confirm that Wnt/beta-catenin signaling is functionally significant to root odontogenesis and cementogenesis during the tooth root development.

语种: 英语
所属项目编号: 81030018 ; 2012CB966904 ; 30900863 ; 81241062
项目资助者: State Key Program of National Natural Science of China ; National Basic Research Program of China ; National Natural Science Foundation of China
WOS记录号: WOS:000316996800001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60902
Appears in Collections:北京大学口腔医学院_口腔病理科_期刊论文

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作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Dept Oral Pathol, Beijing 100081, Peoples R China
2.Inst Biotechnol, Genet Lab Dev & Dis, State Key Lab Prote, Beijing 100071, Peoples R China
3.Zhejiang Univ, Coll Med, Dept Pharmacol, Hangzhou 310003, Zhejiang, Peoples R China

Recommended Citation:
Zhang, Ran,Yang, Guan,Wu, Ximei,et al. Disruption of Wnt/beta-catenin Signaling in Odontoblasts and Cementoblasts Arrests Tooth Root Development in Postnatal Mouse Teeth[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2013,9(3):228-236.
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