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学科主题: 口腔医学
题名:
Abnormal Differentiation of Dental Pulp Cells in Cleidocranial Dysplasia
作者: Yan, W. J.1,2; Zhang, C. Y.1; Yang, X.3; Liu, Z. N.4; Wang, X. Z.1; Sun, X. Y.1; Wang, Y. X.5; Zheng, S. G.1
关键词: cell biology ; craniofacial anomalies ; bone remodeling ; regeneration ; craniofacial biology ; genetics ; osteogenesis ; osteoclasts
刊名: JOURNAL OF DENTAL RESEARCH
发表日期: 2015-04-01
DOI: 10.1177/0022034514566655
卷: 94, 期:4, 页:577-583
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dentistry, Oral Surgery & Medicine
研究领域[WOS]: Dentistry, Oral Surgery & Medicine
关键词[WOS]: PERIODONTAL-LIGAMENT CELLS ; ALTERED GENE-EXPRESSION ; OSTEOBLAST DIFFERENTIATION ; OSTEOCLAST FORMATION ; TOOTH DEVELOPMENT ; DECIDUOUS TEETH ; RUNX2 GENE ; CBFA1 ; BONE ; RESORPTION
英文摘要:

Cleidocranial dysplasia (CCD) is a skeletal dysplasia caused by heterozygous mutations of RUNX2, a gene that is essential for the mineralization of bone and tooth. We isolated primary dental pulp cells from a 10-y-old patient and tested their proliferative capacity, alkaline phosphatase activity, and ability to form mineralized nodules, in comparison with those from 7 healthy children. All these measures were reduced in primary dental pulp cells from the CCD patient. The expression of the osteoblast/odontoblast-associated genes RUNX2, ALP, OCN, and DSPP was also found to be significantly decreased in the primary dental pulp cells of the CCD patient. The osteoclast-related markers TRAP, CTSK, CTR, and MMP9 were decreased in primary dental pulp cells cocultured with human peripheral blood mononuclear cells. Moreover, the expression of RANKL and the ratio of RANKL/OPG were both reduced in the cells from the CCD patient, indicating that the RUNX2 mutation interfered with the bone-remodeling pathway and decreased the capacity of primary dental pulp cells to support osteoclast differentiation. These effects may be partly responsible for the defects in tooth development and the retention of primary teeth that is typical of CCD.

语种: 英语
所属项目编号: 81070815
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000351636500010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60907
Appears in Collections:北京大学口腔医学院_口腔预防保健科_期刊论文

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作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Dept Prevent Dent, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Dept Pediat Dent, Div 1, Beijing 100081, Peoples R China
3.Beijing Univ Chinese Med, Dongzhimen Hosp, Dept Stomatol, Beijing, Peoples R China
4.Peking Univ, Sch & Hosp Stomatol, Dept Prosthodont, Beijing 100081, Peoples R China
5.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100081, Peoples R China

Recommended Citation:
Yan, W. J.,Zhang, C. Y.,Yang, X.,et al. Abnormal Differentiation of Dental Pulp Cells in Cleidocranial Dysplasia[J]. JOURNAL OF DENTAL RESEARCH,2015,94(4):577-583.
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