|Effect of in vitro culture period on birthweight of singleton newborns|
|Zhu, Jinliang1,2,3; Lin, Shengli1,2,3; Li, Ming1,2,3; Chen, Lixue1,2,3; Lian, Ying1,2,3; Liu, Ping1,2,3; Qiao, Jie1,2,3|
|关键词||IVF birthweight blastocyst transfer singleton|
|WOS标题词||Science & Technology|
|类目[WOS]||Obstetrics & Gynecology ; Reproductive Biology|
|研究领域[WOS]||Obstetrics & Gynecology ; Reproductive Biology|
|关键词[WOS]||ASSISTED REPRODUCTIVE TECHNOLOGY ; MESSENGER-RNA EXPRESSION ; DOUBLE-EMBRYO-TRANSFER ; INCREASED RISK ; MOUSE EMBRYOS ; MATERNAL FACTORS ; IMPRINTED GENES ; PRETERM BIRTH ; FERTILIZATION ; IVF|
STUDY QUESTION: Does prolonged in vitro culture influence newborn birthweight?
SUMMARY ANSWER: The absolute mean birthweight and gestational age- and gender-adjusted birthweight (Z scores) of singletons born from blastocyst transfer are higher than singletons born from Day 3 transfer.
WHAT IS KNOWN ALREADY: An increased proportion of large-for-gestational age (LGA) newborns occurs after blastocyst transfer compared with Day 2 transfer, and Z scores for newborns after blastocyst transfer are higher than newborns after transfer on Day 2 or Day 3.
STUDY DESIGN, SIZE AND DURATION: This study was a retrospective analysis of newborn birthweight, including 2929 singletons at the Reproductive Medical Center of Peking University Third Hospital between January 2009 and June 2012. The number of singletons after Day 3 transfer was 2833 and the number of singletons after blastocyst transfer (Day 5-6) was 96.
PARTICIPANTS/MATERIALS, setting, and methods: Only cycles with fresh embryo transfer were included. Patients <= 40 years of age with a BMI < 30 kg/m(2) were analyzed. Only data from singleton newborns born alive after the 20th week of gestation were included in the data analysis. Patients with more than one fetal sac diagnosed by ultrasound but who delivered singletons were excluded. Patients who received PGD and cycles with donor oocytes were excluded. Multiple linear regression analysis was performed to determine the significance of individual factors on absolute birthweight of singleton newborns. The absolute birthweight and Z scores of singletons were compared.
MAIN RESULTS AND THE ROLE OF CHANCE: Multiple linear regression analysis indicated that maternal age, maternal BMI, paternal BMI, type of infertility, gestational age, infant gender and culture period were significantly associated with birthweight. The absolute birthweight for singletons resulting from blastocyst transfer was significantly greater than singletons resulting from Day 3 transfer (3465.31 +/- 51.36 versus 3319.82 +/- 10.04 g respectively, P = 0.009). The Z scores for singletons after blastocyst transfer were significantly higher than singletons after Day 3 transfer (0.347 versus 0.029 respectively, P = 0.016).
LIMITATIONS AND REASONS FOR CAUTION: In our clinic, blastocyst culture is mainly offered to patients with unsuccessful IVF cycles but also to patients with uterine malformations, and therefore this protocol introduced a potential selection bias in our study. Moreover, as certain culture media are associated with fetal overgrowth, the media used may be also a confounding factor, even though the absolute birthweights of singletons were comparable.
WIDER IMPLICATIONS OF THESE FINDINGS: Our study suggests that a prolonged (5-6 days) in vitro culture period has a significant effect on the mean absolute birthweight and Z scores of singleton newborns. The effect of prolonged in vitro culture on epigenetic changes in the embryo needs further study.
|资助机构||National Natural Science Foundation of China for Young Scholars|
|作者单位||1.Minist Educ, Key Lab Assisted Reprod, Beijing, Peoples R China|
2.Peking Univ, Hosp 3, Reprod Med Ctr, Dept Obstet & Gynecol, Beijing 100191, Peoples R China
3.Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing, Peoples R China
|Zhu, Jinliang,Lin, Shengli,Li, Ming,et al. Effect of in vitro culture period on birthweight of singleton newborns[J]. HUMAN REPRODUCTION,2014,29(3):448-454.|
|APA||Zhu, Jinliang.,Lin, Shengli.,Li, Ming.,Chen, Lixue.,Lian, Ying.,...&Qiao, Jie.(2014).Effect of in vitro culture period on birthweight of singleton newborns.HUMAN REPRODUCTION,29(3),448-454.|
|MLA||Zhu, Jinliang,et al."Effect of in vitro culture period on birthweight of singleton newborns".HUMAN REPRODUCTION 29.3(2014):448-454.|