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学科主题: 基础医学
题名:
The specific immune response to tumor antigen CP1 and its correlation with improved survival in colon cancer patients
作者: Liu, Fang-Fang1,2,3; Dong, Xue-Yuan1; Pang, Xue-Wen1; Xing, Qiao1; Wang, Hong-Cheng1; Zhang, Hua-Gang1; Li, Yan1; Yin, Yan-Hui1; Fant, Michael4; Ye, Ying-Jiang2; Shen, Dan-Hua3; Zhang, Yu1; Wang, Shan2; Chen, Wei-Feng1
刊名: GASTROENTEROLOGY
发表日期: 2008-04-01
DOI: 10.1053/j.gastro.2008.01.029
卷: 134, 期:4, 页:998-1006
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: CD8(+) T-CELL ; HUMAN COLORECTAL-CANCER ; CARCINOEMBRYONIC ANTIGEN ; PROTEASOMAL CLEAVAGE ; CARCINOMA PATIENTS ; PLUS IRINOTECAN ; DENDRITIC CELLS ; VACCINATION ; PLACENTA ; PLAC1
英文摘要:

Background & Aims: The present study was undertaken to determine the expression of a newly identified tumor antigen cancer-placenta I (CP1) in colorectal carcinoma (CRC) and explore the CP1-specific immune response in CRC patients and its correlation with patient survival. Methods: CP1 expression was determined by reverse-transcription polymerase chain reaction, immunohistochemistry, and Western blot analysis. Serum antibodies against CP1 were detected by enzyme-linked immunosorbent assay, and T-cell response was measured by interferon-gamma/granzyme-B release enzyme-linked immunospot assays. The HLA-A2-restricted epitopes in CP1 were predicted by bioinformatics and then experimentally validated by enzyme-linked immunospot assay. Results: CP1 expression was detected in a significant number of CRC tissues, reaching 47.6% at the messenger RNA (mRNA) level and 28.6% at the protein level. Of patients with CP1 mRNA(+) tumors, more than 50% had CP1-responsive CD4(+) and CD8(+) T cells and 30% spontaneous-occurring antibodies against CP1. Further studies revealed 2 dominant HLA-A2-restricted epitopes in the CP1 antigen: p31-39 and p58-66. In a follow-up study up to 33 months after surgery, 9 of the 10 patients with CP1-specific CD8 T-cell response survived, whereas 6 of the 8 nonresponders died. Kaplan-Meier analysis indicated a significant correlation between T-cell response and patient survival. Conclusions: CP1 represents a new class of tumor-specific shared antigen. Its high expression in CRC tissues, prevalence of CP1-specific immune responses in CP1 mRNA+ CRC patients, and positive correlation with survival suggest that the antigen may be a useful target for cancer immunotherapy.

语种: 英语
WOS记录号: WOS:000254853800020
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60914
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100083, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Surg Gastroenterol, Surg Oncol Lab, Beijing 100044, Peoples R China
3.Peking Univ, Peoples Hosp, Dept Pathol, Beijing 100871, Peoples R China
4.Univ Texas Houston, Hlth Sci Ctr, Dept Pediat, Houston, TX 77225 USA

Recommended Citation:
Liu, Fang-Fang,Dong, Xue-Yuan,Pang, Xue-Wen,et al. The specific immune response to tumor antigen CP1 and its correlation with improved survival in colon cancer patients[J]. GASTROENTEROLOGY,2008,134(4):998-1006.
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