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学科主题: 临床医学
题名:
The role of calcineurin in the lung fibroblasts proliferation and collagen synthesis induced by basic fibroblast growth factor
作者: Chen, YH; Zhao, MW; Fu, MG; Yao, WZ; Tang, CS
关键词: calcineurin ; lung fibroblast ; collagen ; proliferation ; basic fibroblast growth factor
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2003-06-01
卷: 116, 期:6, 页:857-862
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: SWISS 3T3 FIBROBLASTS ; CYCLOSPORINE-A ; ENDOTHELIN-1 ; EXPRESSION ; ISOFORMS ; TISSUE
英文摘要:

Objective To investigate the role of calcineurin (CaN) in the lung fibroblast proliferation and collagen synthesis induced by basic fibroblast growth factor (bFGF).

Methods We used Western blot and immunohistochemical methods for investigating the content and distribution of calcineurin in the lung tissue. Calcineurin activity in different tissues was measured using P-32-labelled substrate. In the primary culture of lung fibroblasts, H-3-thymidine (H-3-TdR) and H-3-proline incorporation methods were used to study the effect of cyclosporin A ( CsA), an inhibitor of calcineurin, on the lung fibroblast DNA and collagen synthesis stimulated by bFGF.

Results We found that calcineurin was expressed in lung tissue and has phosphatase activity (7.1 +/- 2.0 pmol Pi/mg pr/min). CsA (10(-8) - 10(-6) mol/L) inhibited lung fibroblast H-3-TdR incorporation induced by bFGF in a dose-dependent manner, with the inhibitory rates by 20%, 46% and 66% ( P < 0.01). CsA (10(-7) - 10(-6) mol/L) inhibited H-3-proline incorporation in lung fibroblasts stimulated by bFGF, with the inhibitory rates by 21% and 37% (P < 0.01). In a culture medium, CsA (10(-8) - 10(-6) mol/L) inhibited H-3-proline secretion induced by bFGF in a dose-dependent manner, with the inhibitory rates by 19%, 29% (P < 0.05) and 56% (P < 0.01). CsA (10(-7) mol/L) could inhibit calcineurin activity by 44% in lung fibroblasts ( P < 0.01).

Conclusions Calcineurin is expressed in lung tissue and has phosphatase activity. It is involved in the bFGF stimulated lung fibroblast DNA and collagen synthesis.

语种: 英语
WOS记录号: WOS:000184024900014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60917
Appears in Collections:北京大学第三临床医学院_呼吸内科_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Dept Resp Dis, Beijing 100083, Peoples R China
2.Peking Univ, Teaching Hosp 1, Inst Cardiovasc Res, Beijing 100034, Peoples R China

Recommended Citation:
Chen, YH,Zhao, MW,Fu, MG,et al. The role of calcineurin in the lung fibroblasts proliferation and collagen synthesis induced by basic fibroblast growth factor[J]. CHINESE MEDICAL JOURNAL,2003,116(6):857-862.
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