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The role of calcineurin in the lung fibroblasts proliferation and collagen synthesis induced by basic fibroblast growth factor
Chen, YH; Zhao, MW; Fu, MG; Yao, WZ; Tang, CS
关键词calcineurin lung fibroblast collagen proliferation basic fibroblast growth factor
刊名CHINESE MEDICAL JOURNAL
2003-06-01
116期:6页:857-862
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]SWISS 3T3 FIBROBLASTS ; CYCLOSPORINE-A ; ENDOTHELIN-1 ; EXPRESSION ; ISOFORMS ; TISSUE
英文摘要

Objective To investigate the role of calcineurin (CaN) in the lung fibroblast proliferation and collagen synthesis induced by basic fibroblast growth factor (bFGF).

Methods We used Western blot and immunohistochemical methods for investigating the content and distribution of calcineurin in the lung tissue. Calcineurin activity in different tissues was measured using P-32-labelled substrate. In the primary culture of lung fibroblasts, H-3-thymidine (H-3-TdR) and H-3-proline incorporation methods were used to study the effect of cyclosporin A ( CsA), an inhibitor of calcineurin, on the lung fibroblast DNA and collagen synthesis stimulated by bFGF.

Results We found that calcineurin was expressed in lung tissue and has phosphatase activity (7.1 +/- 2.0 pmol Pi/mg pr/min). CsA (10(-8) - 10(-6) mol/L) inhibited lung fibroblast H-3-TdR incorporation induced by bFGF in a dose-dependent manner, with the inhibitory rates by 20%, 46% and 66% ( P < 0.01). CsA (10(-7) - 10(-6) mol/L) inhibited H-3-proline incorporation in lung fibroblasts stimulated by bFGF, with the inhibitory rates by 21% and 37% (P < 0.01). In a culture medium, CsA (10(-8) - 10(-6) mol/L) inhibited H-3-proline secretion induced by bFGF in a dose-dependent manner, with the inhibitory rates by 19%, 29% (P < 0.05) and 56% (P < 0.01). CsA (10(-7) mol/L) could inhibit calcineurin activity by 44% in lung fibroblasts ( P < 0.01).

Conclusions Calcineurin is expressed in lung tissue and has phosphatase activity. It is involved in the bFGF stimulated lung fibroblast DNA and collagen synthesis.

语种英语
WOS记录号WOS:000184024900014
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60917
专题北京大学第三临床医学院_呼吸科
作者单位1.Peking Univ, Hosp 3, Dept Resp Dis, Beijing 100083, Peoples R China
2.Peking Univ, Teaching Hosp 1, Inst Cardiovasc Res, Beijing 100034, Peoples R China
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GB/T 7714
Chen, YH,Zhao, MW,Fu, MG,et al. The role of calcineurin in the lung fibroblasts proliferation and collagen synthesis induced by basic fibroblast growth factor[J]. CHINESE MEDICAL JOURNAL,2003,116(6):857-862.
APA Chen, YH,Zhao, MW,Fu, MG,Yao, WZ,&Tang, CS.(2003).The role of calcineurin in the lung fibroblasts proliferation and collagen synthesis induced by basic fibroblast growth factor.CHINESE MEDICAL JOURNAL,116(6),857-862.
MLA Chen, YH,et al."The role of calcineurin in the lung fibroblasts proliferation and collagen synthesis induced by basic fibroblast growth factor".CHINESE MEDICAL JOURNAL 116.6(2003):857-862.
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