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学科主题: 基础医学
题名:
Determination of the Four Main Active Compounds and the Chromatographic Fingerprint of Fructus Polygoni Orientalis by HPLC
作者: Tong, Miaomiao1; Wang, Tianmin1; Wang, Yuedan2; Chu, Ming2; Ying, Xixiang1; Li, Na; Zhai, Yanjun1
关键词: Chromatographic fingerprint ; Determination ; Fructus Polygoni orientalis ; HPLC
刊名: LATIN AMERICAN JOURNAL OF PHARMACY
发表日期: 2014-02-01
卷: 33, 期:1, 页:71-77
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: INJURY
英文摘要:

A simple and sensitive reversed-phase, high-performance liquid chromatography method using a diode-array detector (DAD) was developed to evaluate the quality of the fruits of Polygonum orientate L., Polygonaceae (Fructus Polygoni Orientalis, FPO) through its chromatographic fingerprint analysis and a simultaneous analysis of its four compounds, namely taxifolin, 5,7,4′-trihydroxy-dihydroflavonol, p-hydroxyphenyl ethanol p-coumarate, and p-hydroxyphenyl ethanol ferulate. The analysis was accomplished on an Agilent Zorbax SB-C-18 column (150 x 4.6 mu m, 5 iim) using gradient elution with acetonitrile and water. The analyses were performed at 25 C with a flow rate of 1.0 mL/min and using ultraviolet (UV) detection at 290 urn. All of the calibration curves showed a strong regression relationship (r(2) 0.9996) that fell within linear ranges, and their recoveries were in the range of 97.67-103.48%. Twenty common peaks were selected as the characteristic peaks in the chromatographic fingerprint analysis to assess the similarities of 49 FPO samples that were obtained from different geographical areas in China. The results indicated that this developed method was accurate, and that it was successfully applied to the analyses of the four compounds and chromatography fingerprints of FPO.

语种: 英语
所属项目编号: 30873437 ; 20112133110001
项目资助者: National Natural Science Fund of China ; Ph.D. Program Foundation of the Ministry of Education of China
WOS记录号: WOS:000337555200009
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60932
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Liaoning Univ Tradit Chinese Med, Sch Pharm, Dalian 116600, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100191, Peoples R China

Recommended Citation:
Tong, Miaomiao,Wang, Tianmin,Wang, Yuedan,et al. Determination of the Four Main Active Compounds and the Chromatographic Fingerprint of Fructus Polygoni Orientalis by HPLC[J]. LATIN AMERICAN JOURNAL OF PHARMACY,2014,33(1):71-77.
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