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Determination of the Four Main Active Compounds and the Chromatographic Fingerprint of Fructus Polygoni Orientalis by HPLC
Tong, Miaomiao1; Wang, Tianmin1; Wang, Yuedan2; Chu, Ming2; Ying, Xixiang1; Li, Na; Zhai, Yanjun1
关键词Chromatographic fingerprint Determination Fructus Polygoni orientalis HPLC
刊名LATIN AMERICAN JOURNAL OF PHARMACY
2014-02-01
33期:1页:71-77
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]INJURY
英文摘要

A simple and sensitive reversed-phase, high-performance liquid chromatography method using a diode-array detector (DAD) was developed to evaluate the quality of the fruits of Polygonum orientate L., Polygonaceae (Fructus Polygoni Orientalis, FPO) through its chromatographic fingerprint analysis and a simultaneous analysis of its four compounds, namely taxifolin, 5,7,4′-trihydroxy-dihydroflavonol, p-hydroxyphenyl ethanol p-coumarate, and p-hydroxyphenyl ethanol ferulate. The analysis was accomplished on an Agilent Zorbax SB-C-18 column (150 x 4.6 mu m, 5 iim) using gradient elution with acetonitrile and water. The analyses were performed at 25 C with a flow rate of 1.0 mL/min and using ultraviolet (UV) detection at 290 urn. All of the calibration curves showed a strong regression relationship (r(2) 0.9996) that fell within linear ranges, and their recoveries were in the range of 97.67-103.48%. Twenty common peaks were selected as the characteristic peaks in the chromatographic fingerprint analysis to assess the similarities of 49 FPO samples that were obtained from different geographical areas in China. The results indicated that this developed method was accurate, and that it was successfully applied to the analyses of the four compounds and chromatography fingerprints of FPO.

语种英语
WOS记录号WOS:000337555200009
项目编号30873437 ; 20112133110001
资助机构National Natural Science Fund of China ; Ph.D. Program Foundation of the Ministry of Education of China
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文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60932
专题北京大学基础医学院_免疫学系
北京大学基础医学院
作者单位1.Liaoning Univ Tradit Chinese Med, Sch Pharm, Dalian 116600, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100191, Peoples R China
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GB/T 7714
Tong, Miaomiao,Wang, Tianmin,Wang, Yuedan,et al. Determination of the Four Main Active Compounds and the Chromatographic Fingerprint of Fructus Polygoni Orientalis by HPLC[J]. LATIN AMERICAN JOURNAL OF PHARMACY,2014,33(1):71-77.
APA Tong, Miaomiao.,Wang, Tianmin.,Wang, Yuedan.,Chu, Ming.,Ying, Xixiang.,...&Zhai, Yanjun.(2014).Determination of the Four Main Active Compounds and the Chromatographic Fingerprint of Fructus Polygoni Orientalis by HPLC.LATIN AMERICAN JOURNAL OF PHARMACY,33(1),71-77.
MLA Tong, Miaomiao,et al."Determination of the Four Main Active Compounds and the Chromatographic Fingerprint of Fructus Polygoni Orientalis by HPLC".LATIN AMERICAN JOURNAL OF PHARMACY 33.1(2014):71-77.
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