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学科主题: 临床医学
题名:
Differential proteomic analysis of neonatal cardiomyocytes in response to beta-adrenergic receptor stimulation
作者: Li, ZJ; Liu, N; Liu, ZQ; Liu, SY; Han, QD; Zhang, YY
关键词: proteome ; two-dimensional electrophoresis(2-DE) ; MALDI-MS ; beta-adrenoceptor ; cardiomyocyte
刊名: CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
发表日期: 2006-05-01
卷: 22, 期:3, 页:319-323
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
关键词[WOS]: MASS-SPECTROMETRY ; PROTEINS ; IDENTIFICATION ; GENES
英文摘要:

beta-Adrenoceptors(beta-ARs) play a critical role in regulating cardiac functions under both physiological and pathological conditions. To further explore the mechanisms through which beta-ARs perform its actions, proteomic approaches were adopted to study the global protein patterns in cultured neonatal rat cardiomyocytes exposed to isoproterenol (ISO). A modified method, "Mirror Images in One Gel", was used to improve the reproducibility and resolution power of two-dimensional electrophoresis. A 2-DE map with a good reproducibility was obtained in which 1281 70 spots were detected and about 1191 +/- 54 spots were matched, with an average matching rate of 92.9%. Nine proteins with significant changes were identified by using peptide mass fingerprinting(PMF) data obtained via MALDI-MS.

语种: 英语
WOS记录号: WOS:000237999200012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60936
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

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作者单位: 1.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100083, Peoples R China
2.Peking Univ Third Hosp, Inst Vasc Med, Beijing 100083, Peoples R China
3.Chinese Acad Sci, Changchun Inst Appl Chem, Changchun 130022, Peoples R China

Recommended Citation:
Li, ZJ,Liu, N,Liu, ZQ,et al. Differential proteomic analysis of neonatal cardiomyocytes in response to beta-adrenergic receptor stimulation[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2006,22(3):319-323.
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