IR@PKUHSC  > 北京大学第三临床医学院  > 心血管内科
学科主题临床医学
Differential proteomic analysis of neonatal cardiomyocytes in response to beta-adrenergic receptor stimulation
Li, ZJ; Liu, N; Liu, ZQ; Liu, SY; Han, QD; Zhang, YY
关键词proteome two-dimensional electrophoresis(2-DE) MALDI-MS beta-adrenoceptor cardiomyocyte
刊名CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
2006-05-01
22期:3页:319-323
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]MASS-SPECTROMETRY ; PROTEINS ; IDENTIFICATION ; GENES
英文摘要

beta-Adrenoceptors(beta-ARs) play a critical role in regulating cardiac functions under both physiological and pathological conditions. To further explore the mechanisms through which beta-ARs perform its actions, proteomic approaches were adopted to study the global protein patterns in cultured neonatal rat cardiomyocytes exposed to isoproterenol (ISO). A modified method, "Mirror Images in One Gel", was used to improve the reproducibility and resolution power of two-dimensional electrophoresis. A 2-DE map with a good reproducibility was obtained in which 1281 70 spots were detected and about 1191 +/- 54 spots were matched, with an average matching rate of 92.9%. Nine proteins with significant changes were identified by using peptide mass fingerprinting(PMF) data obtained via MALDI-MS.

语种英语
WOS记录号WOS:000237999200012
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60936
专题北京大学第三临床医学院_心血管内科
作者单位1.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100083, Peoples R China
2.Peking Univ Third Hosp, Inst Vasc Med, Beijing 100083, Peoples R China
3.Chinese Acad Sci, Changchun Inst Appl Chem, Changchun 130022, Peoples R China
推荐引用方式
GB/T 7714
Li, ZJ,Liu, N,Liu, ZQ,et al. Differential proteomic analysis of neonatal cardiomyocytes in response to beta-adrenergic receptor stimulation[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2006,22(3):319-323.
APA Li, ZJ,Liu, N,Liu, ZQ,Liu, SY,Han, QD,&Zhang, YY.(2006).Differential proteomic analysis of neonatal cardiomyocytes in response to beta-adrenergic receptor stimulation.CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,22(3),319-323.
MLA Li, ZJ,et al."Differential proteomic analysis of neonatal cardiomyocytes in response to beta-adrenergic receptor stimulation".CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 22.3(2006):319-323.
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