IR@PKUHSC  > 北京大学口腔医学院
学科主题口腔医学
Claudin-4 is required for AMPK-modulated paracellular permeability in submandibular gland cells
Xiang, Ruo-Lan1,2; Mei, Mei3; Cong, Xin1,2; Li, Jing3; Zhang, Yan1,2; Ding, Chong4; Wu, Li-Ling1,2; Yu, Guang-Yan3
关键词AMP-activated protein kinase claudin-4 tight junction paracellular permeability submandibular gland
刊名JOURNAL OF MOLECULAR CELL BIOLOGY
2014-12-01
DOI10.1093/jmcb/mju048
6期:6页:486-497
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]TIGHT JUNCTION PROTEINS ; EPITHELIAL-CELLS ; SALIVARY-GLANDS ; BARRIER FUNCTION ; OCCLUDIN ; KINASE ; PHOSPHORYLATION ; ACTIVATION ; EXPRESSION ; MONOLAYERS
英文摘要

Tight junction plays an important role in mediating paracellular permeability in epithelia. We previously found that activation of AMP-activated protein kinase (AMPK) increased saliva secretion by modulating paracellular permeability in submandibular glands. However, the molecular mechanisms underlying AMPK-modulated paracellular permeability are unknown. In this study, we found that AICAR, an AMPK agonist, increased saliva secretion in the isolated rat submandibular glands, decreased transepithelial electrical resistance (TER), and increased 4 kDa FITC-dextran flux in cultured SMG-C6 cells. AICAR also induced redistribution of tight junction protein claudin-4, but not claudin-1, claudin-3, occludin, or ZO-1, from the cytoplasm to the membrane. Moreover, knockdown of claudin-4 by shRNA suppressed while claudin-4 re-expression restored the TER and 4 kDa FITC-dextran flux responses to AICAR. Additionally, AICAR increased ERK1/2 phosphorylation, and inhibition of ERK1/2 by U0126, an ERK1/2 kinase inhibitor, or by siRNA decreased AICAR-induced TER responses. AICAR induced the serine S199 phosphorylation of claudin-4 and enhanced the interaction of claudin-4 and occludin. Furthermore, pretreatment with U0126 significantly suppressed AMPK-modulated phosphorylation, redistribution, and interaction with occludin of claudin-4. Taken together, these results indicated that claudin-4 played a crucial role in AMPK-modulated paracellular permeability and ERK1/2 was required in AMPK-modulated tight junction barrier function in submandibular gland.

语种英语
WOS记录号WOS:000348600100005
项目编号81100763 ; 81271161 ; 81442007
资助机构National Natural Science Foundation of China
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60937
专题北京大学口腔医学院
北京大学基础医学院
北京大学口腔医学院_外科
北京大学口腔医学院_中心实验室
作者单位1.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Ctr Salivary Gland Dis,Sch & Hosp Stomatol, Dept Physiol & Pathophysiol,Sch Basic Med Sci, Beijing 100191, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100081, Peoples R China
4.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100081, Peoples R China
推荐引用方式
GB/T 7714
Xiang, Ruo-Lan,Mei, Mei,Cong, Xin,et al. Claudin-4 is required for AMPK-modulated paracellular permeability in submandibular gland cells[J]. JOURNAL OF MOLECULAR CELL BIOLOGY,2014,6(6):486-497.
APA Xiang, Ruo-Lan.,Mei, Mei.,Cong, Xin.,Li, Jing.,Zhang, Yan.,...&Yu, Guang-Yan.(2014).Claudin-4 is required for AMPK-modulated paracellular permeability in submandibular gland cells.JOURNAL OF MOLECULAR CELL BIOLOGY,6(6),486-497.
MLA Xiang, Ruo-Lan,et al."Claudin-4 is required for AMPK-modulated paracellular permeability in submandibular gland cells".JOURNAL OF MOLECULAR CELL BIOLOGY 6.6(2014):486-497.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Claudin-4 is require(1188KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Xiang, Ruo-Lan]的文章
[Mei, Mei]的文章
[Cong, Xin]的文章
百度学术
百度学术中相似的文章
[Xiang, Ruo-Lan]的文章
[Mei, Mei]的文章
[Cong, Xin]的文章
必应学术
必应学术中相似的文章
[Xiang, Ruo-Lan]的文章
[Mei, Mei]的文章
[Cong, Xin]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Claudin-4 is required for AMPK-modulated paracellular permeability in submandibular gland cells.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。